In this paper, we formulate a regular q-fractional Sturm-Liouville problem (qF-SLP) which includes the left-sided Riemann-Liouville and the right-sided Caputo q-fractional derivatives of the same order α, α ∈ (0, 1). The properties of the eigenvalues and the eigenfunctions are investigated. A q-fractional version of the Wronskian is defined and its relation to the simplicity of the eigenfunctions is verified. We use the fixed point theorem to introduce a sufficient condition on eigenvalues for the existence and uniqueness of the associated eigenfunctions when α > 1/2. These results are a generalization of the integer regular q-SturmLiouville problem introduced by Annaby and Mansour in [1]. An example for a qFSLP whose eigenfunctions are little q-Jacobi polynomials is introduced.Keywords: Left and right sided Riemann-Liouville and Caputo q-derivatives, eigenvalues and eigenfunctions, existence and uniqueness theorem, q, α Wronskian. 2000 MSC: 39A13, 26A33, 34L10.