Extration, identification and stability ananlysis of anthocyanins from organic Guizhou blueberries in China

Abstract

Guizhou Majiang organic blueberries from China are a prevalent fruit in the health industry and have high anthocyanin contents. This study used the ethanol extraction method, ultrasonic-assisted extraction, AB 8 macroporous resin adsorption purification, and the addition of 3% citric acid to extract anthocyanins, and through single-factor tests and orthogonal tests, the optimum extraction conditions of anthocyanins from blueberries was determined. The optimal extraction conditions were as follows: the extraction temperature was 40 °C, the ethanol volume fraction was 80%, the solid-liquid ratio was 1:10, the ultrasonic extraction time was 60 min, and the extraction amount was 2.56 mg/g. Anthocyanins were identified by HPLC, and the effects of storage conditions on their contents were investigated. K + , Cu 2+ , and Fe 2+ had little influence on anthocyanin contents, while hydrogen peroxide had a highly destructive effect. With increasing illumination time, the retention rate of anthocyanins decreased gradually; the higher the temperature was, the stronger the damaging effect on anthocyanin contents, and the appropriate storage temperature was determined to be -4 °C or below. The absorbance was the highest when pH =2. Taken together, the results of our study revealed the optimal extraction process and appropriate preservation conditions of anthocyanins from organic Guizhou blueberries.

Comparison of pulse electric field, microwave and ultrasonic pretreatment prior to black rice extraction on antioxidant and sirtuin1 enzyme stimulating activities

“…Ultrasound waves during UAE are attributed to mechanical effects of cell disruption on black rice pericarp, and increased solvent diffusion leading to enhance ultrasonic capillary effect (Thakur et al, 2022). These effects are similar to the effects UAE on other plant materials (Yin et al, 2022;Zhong & Wang, 2010). Pham et al (2022) demonstrated that the UAE requires additional time when compared to the effectiveness of MAE.…”

Preparation of Blueberry Anthocyanin Nanoemulsions and Their Stability and Skin Safety Evaluation

“…Anthocyanins were extracted from organic blueberries produced in Majang County, Guizhou (China). The research group had completed this work in the early stage, and the content of anthocyanins in blueberries was 2.56 mg/g [14], the extracted blueberry anthocyanins were turned into lyophilized powder by vacuum freeze-drying.…”

Effect of environmental factors on the polysaccharide content of Dendrobium officinale

“…In recent years, the R&D of natural functional products from herbs has attracted more and more attention in the world (Ruiz-Cisneros et al, 2022;Wang et al, 2022a, b;Yin et al, 2022). The genus Dendrobium is one of the largest genera in the orchid family, containing 1500-2000 species (Hou et al, 2017).…”