Extravascular Distribution of Fluorescent Albumin, Globulin and Fibrinogen in Connective Tissue Structures

Mancini, R. E.; Vilar, O.; Dellacha, Juan M.; Davidson, O. W.; Gómez, César Cinesi; Álvarez, Beatriz

doi:10.1177/10.2.194

Cited by 42 publications

(9 citation statements)

References 5 publications

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“…Such permeability has been attributed to the basal membrane of the granulosa layer (Mansini et al, 1962). In addition, these results provide strong support for the conclusion of previous researchers (e.g.…”

Section: Discussionsupporting

confidence: 80%

Some Chemical, Immunochemical and Electrophoretic Properties of Bovine Follicular Fluid

Desjardins¹,

Kirton²,

Hafs³

1966

Reproduction

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Bovine follicular fluid was compared with blood serum or blood plasma with respect to the concentration of protein and free amino nitrogen, the number of antigens and the number and mobilities of electrophoretic components. The protein concentration of follicular fluid (7\m=.\08g/100 ml) was less than that of blood serum (9\m=.\10g/100 ml), but no significant difference existed between the values of free amino nitrogen in the two fluids (4\m=.\31 and 3\m=.\97 mg/100 ml, respectively).Rabbit antisera to follicular fluid, blood serum or blood plasma gave several precipitin lines (at least 7, 7 and 8 respectively) when reacted with their homologous antigens in agar\p=n-\gel-diffusion studies. After absorption with blood serum, there was one antigen (presumably fibrinogen) in blood plasma and follicular fluid that was not found in blood serum. These results were substantiated by immuno-electrophoresis.Moving boundary electrophoresis in five different buffers revealed at least eight components in follicular fluid and blood serum and at least nine in blood plasma. Minor differences were observed in the electrophoretic mobilities of some components found in both follicular fluid and blood serum.The results showed that the major macromolecular components of follicular fluid and of blood were similar, but that some minor macromolecular ones may differ.

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Section: Discussionsupporting

confidence: 80%

Some Chemical, Immunochemical and Electrophoretic Properties of Bovine Follicular Fluid

Desjardins¹,

Kirton²,

Hafs³

1966

Reproduction

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“…The uptake of the two fluorescent proteins by cells of those normal tissues examined was very similar to that observed by other investigators (Kruse and McMaster, 1949;Schiller, Schayer and Hess, 1952;Gitlin, Landing and Whipple, 1953;Mancini et al, 1962), and was chiefly confined to cells of the reticuloendothelial system. Fluorescent protein was also occasionally seen within proximal tubule cells of the kidney.…”

Section: Discussionsupporting

confidence: 71%

“…During homogenization of the tumour, desorption of intact labelled proteins from the tumour stroma could occur. The binding of the labelled proteins by fibres was not solely dependent on the presence of the label, since Mancini et al (1962) obtained identical results using both the directly labelled proteins and fluorescent antibodies.…”

Section: Discussionsupporting

confidence: 56%

The Uptake of Fluorescent Labelled Proteins by Normal and Tumour Tissues In Vivo

Easty¹

1964

Br J Cancer

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INVESTIGATIONS of the uptake of intact proteins by tumour cells in vivo have yielded conflicting results (Busch, Fujiwara and Firszt, 1961;Babson and Winnick, 1954;Campbell and Stone, 1957). In this study use was made of fluorescent labelled proteins as in the previous in vitro work (Easty, Yarnell and Andrews, 1964). The results obtained with a variety of tumours are discussed in relation to the uptake by normal tissues, the access of blood-borne substances to the tumours, and the detailed localization of protein within normal and tumour tissues. MATERIALS AND METHODS Preparation and Injection offluorescent proteinsTwo proteins were used, crystallized bovine plasma albumin (Armour) and purified diphtheria toxoid (kindly supplied by Dr. C. G. Pope of the Wellcome Foundation), which were labelled with fluorescein isothiocyanate as in previous experiments (Easty et al., 1964). The final concentrations of fluorescent proteins were adjusted to 5 per cent on 0-85 per cent saline. The usual route of injection was the tail vein of rats and mice, and the jugular vein of hamsters. The intraperitoneal as well as the intravenous route of injection was used for mice bearing ascites tumours. Mice were injected with 05 ml. of the protein solutions, rats received 2 ml. and hamsters 1 ml. Animals were killed at intervals after injection varying from several minutes to 4 days, but mostly after 6 hours. Samples of the liver, kidneys, lung, spleen, inguinal lymph nodes, skin and bone marrow from the femurs, as well as the tumours were removed for examination. Preparations of sectionsSeveral methods of processing the tissues after their removal from the animal were tried. Some samples were frozen using solid C02, ethyl alcohol mixtures and then sectioned with a freezing microtome. Others were fixed for several hours in neutralized 4 per cent formaldehyde, then sectioned with a freezing microtome. The method finally chosen gave the highest resolution with little loss of the fluorescence of the injected proteins, and involved fixation with neutralized 4 per cent formaldehyde in 085 per cent saline for 24 hours followed by paraffin-wax embedding, sectioning, and mounting without deparaffinization in D.P.X. The paraffin-wax, D.P.X. and all solvents used were checked to ensure that they were free of fluorescent contaminants which might stain-the sections. Sections of

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“…Before use, labelled antisera were absorbed with guinea-pig liver powder. Where weak reactions were obtained by the " direct " technique, the results were confirmed by the " indirect " method (Nairn, 1964), with the specific sheep anti-rabbit-yG-globulin as the h a 1 anti-globulin reagent.…”

mentioning

confidence: 87%

The pathogenesis of atherosclerosis of the mitral and aortic valves

Walton

Williamson

Johnson

1970

The Journal of Pathology

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Extravascular Distribution of Fluorescent Albumin, Globulin and Fibrinogen in Connective Tissue Structures

Cited by 42 publications

References 5 publications

Some Chemical, Immunochemical and Electrophoretic Properties of Bovine Follicular Fluid

Some Chemical, Immunochemical and Electrophoretic Properties of Bovine Follicular Fluid

The Uptake of Fluorescent Labelled Proteins by Normal and Tumour Tissues In Vivo

The pathogenesis of atherosclerosis of the mitral and aortic valves

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