Extreme Environments and High-Level Bacterial Tellurite Resistance

Maltman, Chris; Yurkov, Vladimir

doi:10.3390/microorganisms7120601

Cited by 25 publications

(22 citation statements)

References 180 publications

(233 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Interest in the development of bioremediation and recovery strategies for tellurium, as well as for other metals and metalloids, has thus increased. In turn, the global demand for this element in technology manufacture is driving a search for new environmentally compatible tools for the recovery of tellurium from its natural sources [3].…”

Section: Introductionmentioning

confidence: 99%

The putative phosphate transporter PitB (PP1373) is involved in tellurite uptake in Pseudomonas putida KT2440

Montenegro¹,

Vieto²,

Emmenegger

et al. 2021

Microbiology

View full text Add to dashboard Cite

Tellurium oxyanions are chemical species of great toxicity and their presence in the environment has increased because of mining industries and photovoltaic and electronic waste. Recovery strategies for this metalloid that are based on micro-organisms are of interest, but further studies of the transport systems and enzymes responsible for implementing tellurium transformations are required because many mechanisms remain unknown. Here, we investigated the involvement in tellurite uptake of the putative phosphate transporter PitB (PP1373) in soil bacterium Pseudomonas putida KT2440. For this purpose, through a method based on the CRISPR/Cas9 system, we generated a strain deficient in the pitB gene and characterized its phenotype on exposing it to varied concentrations of tellurite. Growth curves and transmission electronic microscopy experiments for the wild-type and ΔpitB strains showed that both were able to internalize tellurite into the cytoplasm and reduce the oxyanion to black nano-sized and rod-shaped tellurium particles, although the ΔpitB strain showed an increased resistance to the tellurite toxic effects. At a concentration of 100 μM tellurite, where the biomass formation of the wild-type strain decreased by half, we observed a greater ability of ΔpitB to reduce this oxyanion with respect to the wild-type strain (~38 vs ~16 %), which is related to the greater biomass production of ΔpitB and not to a greater consumption of tellurite per cell. The phenotype of the mutant was restored on over-expressing pitB in trans. In summary, our results indicate that PitB is one of several transporters responsible for tellurite uptake in P. putida KT2440.

show abstract

Section: Introductionmentioning

confidence: 99%

The putative phosphate transporter PitB (PP1373) is involved in tellurite uptake in Pseudomonas putida KT2440

Montenegro¹,

Vieto²,

Emmenegger

et al. 2021

Microbiology

View full text Add to dashboard Cite

show abstract

“…Interest has hence increased in the development of bioremediation and recovery strategies for tellurium as well as for other metals and metalloids. In turn, the global demand for this element in technology manufacture, drives a search for new environmentally compatible tools for the recovery of this element from its natural sources [3].…”

Section: Introductionmentioning

confidence: 99%

The putative phosphate transporter PitB (PP1373) is involved in tellurite uptake in Pseudomonas putida KT2440

Montenegro¹,

Vieto²,

Wicki-Emmenegger

et al. 2020

Preprint

View full text Add to dashboard Cite

Tellurium oxyanions are chemical species with great toxicity; their presence in the environment has increased because of mining industries and photovoltaic and electronic waste. Recovery strategies based on microorganisms for this metalloid are of interest, but further studies of the transport systems and enzymes responsible for implementing tellurium transformations are required because many mechanisms remain unknown. Here, we investigated the involvement in tellurite uptake of the putative phosphate transporter PitB (PP1373) in soil bacterium Pseudomonas putida KT2440. For this purpose, through a method based on the CRISPR/Cas9 system, we generated a strain deficient in pitB gene and characterized its phenotype on exposing it to varied concentrations of tellurite. Growth curves and Transmission Electronic Microscopy experiments of wild type and ΔpitB showed that both strains were able to internalize tellurite into the cytoplasm and reduce the oxyanion to black nano-sized and rod-shaped tellurium particles, however, ΔpitB strain showed an increased resistance to the tellurite toxic effects. At a concentration of 100 uM tellurite, where the biomass formation of wild type strain decreased by half, we observed a greater ability of ΔpitB to reduce this oxyanion with respect to wild type strain (~38% vs ~16%), which is related by the greater biomass production of ΔpitB and not by a greater consumption of tellurite per cell. The phenotype of the mutant was restored on over-expressing pitB in trans. In summary, our results indicate that PitB is one of several transporters responsible for tellurite uptake in P. putida KT2440.

show abstract

“…They can be specific, whereby the main role of the cell is to resist the action of a toxic compound, or nonspecific, in which the resistance is based on a component that is involved in other cellular functions but that also exerts a protective effect against the toxicant. The nonspecific mechanisms are carried out at low levels of resistance/reduction [ 42 ] and can be enzymatic or non-enzymatic. Resistance can be achieved by mutations that affect the intracellular target for a given antimicrobial drug.…”

Section: Effects Of Tellurium and Its Compounds On Selected Prokaryotic Systemsmentioning

confidence: 99%

Tellurium: A Rare Element with Influence on Prokaryotic and Eukaryotic Biological Systems

Vávrová

Struhárňanská

Turña

et al. 2021

IJMS

View full text Add to dashboard Cite

Metalloid tellurium is characterized as a chemical element belonging to the chalcogen group without known biological function. However, its compounds, especially the oxyanions, exert numerous negative effects on both prokaryotic and eukaryotic organisms. Recent evidence suggests that increasing environmental pollution with tellurium has a causal link to autoimmune, neurodegenerative and oncological diseases. In this review, we provide an overview about the current knowledge on the mechanisms of tellurium compounds’ toxicity in bacteria and humans and we summarise the various ways organisms cope and detoxify these compounds. Over the last decades, several gene clusters conferring resistance to tellurium compounds have been identified in a variety of bacterial species and strains. These genetic determinants exhibit great genetic and functional diversity. Besides the existence of specific resistance mechanisms, tellurium and its toxic compounds interact with molecular systems, mediating general detoxification and mitigation of oxidative stress. We also discuss the similarity of tellurium and selenium biochemistry and the impact of their compounds on humans.

show abstract

Extreme Environments and High-Level Bacterial Tellurite Resistance

Cited by 25 publications

References 180 publications

The putative phosphate transporter PitB (PP1373) is involved in tellurite uptake in Pseudomonas putida KT2440

The putative phosphate transporter PitB (PP1373) is involved in tellurite uptake in Pseudomonas putida KT2440

The putative phosphate transporter PitB (PP1373) is involved in tellurite uptake in Pseudomonas putida KT2440

Tellurium: A Rare Element with Influence on Prokaryotic and Eukaryotic Biological Systems

Contact Info

Product

Resources

About