Extremely High Two-Photon Absorbing Graphene Oxide for Imaging of Tumor Cells in the Second Biological Window

Pramanik, Avijit; Chavva, Suhash Reddy; Fan, Zhen; Sinha, Sudarson Sekhar; Nellore, Bhanu Priya Viraka; Ray, Paresh Chandra

doi:10.1021/jz5009856

Cited by 47 publications

(95 citation statements)

References 45 publications

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“…In last one decade, due to the huge advances in the innovative developments of laser systems, detector devices and optical filter design, we are now able to use the combination of several NLO modalities for advancing biological imaging into a single microscope platform, which is known as multimodal NLO imaging 21–30 . For in vivo imaging using noninvasive technology, near infrared (NIR) light between 650–950 nm (biological I window) and 1000–1350 nm (biological II window) needs to be used for providing maximum radiation penetration through tissue 1–5,11–13,31–33 . To date, most of the cell imaging is reported using biological I window (650–950 nm) NIR light, although it is well documented that due to the substantial background noise from tissue autofluorescence and the tissue penetration depth is limited to 1–2 cm, biological I window is not optimal 1–5,11–13 .…”

Section: Introductionmentioning

confidence: 99%

Multimodal Nonlinear Optical Imaging of Live Cells Using Plasmon-Coupled DNA-Mediated Gold Nanoprism Assembly

et al. 2016

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Multiphoton excitation microscopy techniques are the emerging nonlinear optical (NLO) imaging methods to watch the biological world due its ability to penetrate deep into living tissues. Driven by the need to develop multimodal NLO imaging probe, current article reports the design of DNA-mediated gold nanoprisms assembly based optical antennas to enhance multiphoton imaging capability in biological II window. Reported experimental data show a unique way to enhance second harmonic generation (SHG) and two-photon fluorescence (TPF) properties by several orders of magnitudes via plasmon coupled organization into gold nanoprism assembly structures. Experimental and theoretical modeling data using finite difference time domain (FDTD) simulations indicate that huge enhancement of SHG and TPF properties are mainly due to the electric quadrupole contribution and electric field enhancement. Using 1100 nm biological II window light, reported results demonstrated that antibody conjugated assembly structures are capable of exhibiting highly selective and very bright multimodal SHG and TPF imaging of human Hep G2 liver cancer cells.

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Section: Introductionmentioning

confidence: 99%

Multimodal Nonlinear Optical Imaging of Live Cells Using Plasmon-Coupled DNA-Mediated Gold Nanoprism Assembly

et al. 2016

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“…The unique physical, chemical, electronic, thermal, and mechanical properties of this carbon-based 2D nanomaterial have attracted interest in biomedicine, such as cancer molecular imaging2, enzyme adsorption3, biosensors4, and drug delivery56. Graphene oxide (GO) is a layered material consisting of hydrophilic oxygenated graphene sheets bearing oxygen functional groups on their basal planes and edges, which facilitates conjugation of proteins7, DNAs/RNAs89 or chemical drugs10.…”

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confidence: 99%

In vitro and in vivo comparison of the immunotoxicity of single- and multi-layered graphene oxides with or without pluronic F-127

Cho

Pak

Joo

et al. 2016

Sci Rep

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Graphene oxide (GO) has been a focus of research in the fields of electronics, energy, and biomedicine, including drug delivery. Thus, single- and multi-layered GO (SLGO and MLGO) have been produced and investigated. However, little information on their toxicity and biocompatibility is available. In the present study, we performed a comprehensive study of the size- and dose-dependent toxicity of GOs in the presence or absence of Pluronic F-127 on THP-1 cells by examining their viability, membrane integrity, levels of cytokine and ROS production, phagocytosis, and cytometric apoptosis. Moreover, as an extended study, a toxicity evaluation in the acute and chronic phases was performed in mice via intravenous injection of the materials. GOs exhibited dose- and size-dependent toxicity. Interestingly, SLGO induced ROS production to a lesser extent than MLGO. Cytometric analysis indicated that SLGO induced necrosis and apoptosis to a lesser degree than MLGO. In addition, cell damage and IL-1β production were influenced by phagocytosis. A histological animal study revealed that GOs of various sizes induced acute and chronic damage to the lung and kidney in the presence or absence of Pluronic F-127. These results will facilitate studies of GO prior to its biomedical application.

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“…10–16 Despite huge advances in discovering various types of fluorescence probes, single-photon fluorescence imaging for biomolecules using second biological NIR light remains a huge challenge. 15–21 Two-photon luminescence (TPF) imaging has been introduced in biology and clinical study to solve the above problem. 15–24 But, finding photostable TPF material that exhibits strong two-photon luminescence efficiency in biological window II is rare.…”

Section: Introductionmentioning

confidence: 99%

“…15–21 Two-photon luminescence (TPF) imaging has been introduced in biology and clinical study to solve the above problem. 15–24 But, finding photostable TPF material that exhibits strong two-photon luminescence efficiency in biological window II is rare. 20–28 The current article reports plasmon-coupling enhanced, bright two-photon imaging of melanoma UACC903 cells in biological II window using anti-GD2 antibody attached gold nanoparticle (GNP) conjugated single-wall carbon nanotubes (SWCNTs).…”

Section: Introductionmentioning

confidence: 99%

“…4–7,11,15,17–22 As a result, aptamer/antibody or peptide-conjugated gold nanoparticles are very good candidates for bioimaging in clinical environment. 4–7,11,15,17–22 Similarly, we and others have reported that, due to high yield production at low cost, carbon nanomaterials like SWCNTs hold great promise for various applications for our society. 8–10,12,23,24 Since spherical gold nanoparticles do not have absorption in the second biological window, here we have used two-photon luminescence spectroscopy to image melanoma cell selectively.…”

Section: Introductionmentioning

confidence: 99%

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Hybrid Theranostic Platform for Second Near-IR Window Light Triggered Selective Two-Photon Imaging and Photothermal Killing of Targeted Melanoma Cells

Tchounwou

Sinha

Nellore

et al. 2015

ACS Appl. Mater. Interfaces

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Despite advances in the medical field, even in the 21st century cancer is one of the leading causes of death for men and women in the world. Since the second near-infrared (NIR) biological window light between 950 and 1350 nm offers highly efficient tissue penetration, the current article reports the development of hybrid theranostic platform using anti-GD2 antibody attached gold nanoparticle (GNP) conjugated, single-wall carbon nanotube (SWCNT) for second near-IR light triggered selective imaging and efficient photothermal therapy of human melanoma cancer cell. Reported results demonstrate that due to strong plasmon-coupling, two-photon luminescence (TPL) intensity from theranostic GNP attached SWCNT materials is 6 orders of magnitude higher than GNP or SWCNT alone. Experimental and FDTD simulation data indicate that the huge enhancement of TPL intensity is mainly due to strong resonance enhancement coupled with the stronger electric field enhancement. Due to plasmon coupling, the theranostic material serves as a local nanoantennae to enhance the photothermal capability via strong optical energy absorption. Reported data show that theranostic SWCNT can be used for selective two-photon imaging of melanoma UACC903 cell using 1100 nm light. Photothermal killing experiment with 1.0 W/cm2 980 nm laser light demonstrates that 100% of melanoma UACC903 cells can be killed using theranostic SWCNT bind melanoma cells after just 8 min of exposure. These results demonstrate that due to plasmon coupling, the theranostic GNP attached SWCNT material serves as a two-photon imaging and photothermal source for cancer cells in biological window II.

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Extremely High Two-Photon Absorbing Graphene Oxide for Imaging of Tumor Cells in the Second Biological Window

Cited by 47 publications

References 45 publications

Multimodal Nonlinear Optical Imaging of Live Cells Using Plasmon-Coupled DNA-Mediated Gold Nanoprism Assembly

Multimodal Nonlinear Optical Imaging of Live Cells Using Plasmon-Coupled DNA-Mediated Gold Nanoprism Assembly

In vitro and in vivo comparison of the immunotoxicity of single- and multi-layered graphene oxides with or without pluronic F-127

Hybrid Theranostic Platform for Second Near-IR Window Light Triggered Selective Two-Photon Imaging and Photothermal Killing of Targeted Melanoma Cells

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