Extremely Rapid Extraction of DNA from Bacteria and Yeasts

Cheng, Hairong; Jiang, Ning

doi:10.1007/s10529-005-4688-z

Cited by 283 publications

(145 citation statements)

References 11 publications

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“…For taxonomic identification of this yeast strain, genomic DNA was extracted according to the simple phenol lysis method [13] to amplify partial sequences of its 18S rDNA and cytochrome c oxidase subunit 2 genes. The primers used for amplification of 18 S rDNA were: 5'-ATC CTG CCA GTA GTC ATA TGC TTG TCT C-3' and 5'-GAG GCC TCA CTA AGC CAT TCA ATC GGT A-3'; while those for the cytochrome c oxidase subunit 2 gene were: 5'-AATATAATGTTTTATTTAGTATTAATA-3' and 5'-TTTGATAGGCATCGCACTATGAGC-3'.…”

Section: Methodsmentioning

confidence: 99%

A novel method to prepare L-Arabinose from xylose mother liquor by yeast-mediated biopurification

Cheng

Wang

et al. 2011

Microb Cell Fact

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BackgroundL-arabinose is an important intermediate for anti-virus drug synthesis and has also been used in food additives for diets-controlling in recent years. Commercial production of L-arabinose is a complex progress consisting of acid hydrolysis of gum arabic, followed by multiple procedures of purification, thus making high production cost. Therefore, there is a biotechnological and commercial interest in the development of new cost-effective and high-performance methods for obtaining high purity grade L-arabinose.ResultsAn alternative, economical method for purifying L-arabinose from xylose mother liquor was developed in this study. After screening 306 yeast strains, a strain of Pichia anomala Y161 was selected as it could effectively metabolize other sugars but not L-arabinose. Fermentation in a medium containing xylose mother liquor permitted enrichment of L-arabinose by a significant depletion of other sugars. Biochemical analysis of this yeast strain confirmed that its poor capacity for utilizing L-arabinose was due to low activities of the enzymes required for the metabolism of this sugar. Response surface methodology was employed for optimization the fermentation conditions in shake flask cultures. The optimum conditions were: 75 h fermentation time, at 32.5°C, in a medium containing 21% (v/v) xylose mother liquor. Under these conditions, the highest purity of L-arabinose reached was 86.1% of total sugar, facilitating recovery of white crystalline L-arabinose from the fermentation medium by simple methods.ConclusionYeast-mediated biopurification provides a dynamic method to prepare high purity of L-arabinose from the feedstock solution xylose mother liqour, with cost-effective and high-performance properties.

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Section: Methodsmentioning

confidence: 99%

A novel method to prepare L-Arabinose from xylose mother liquor by yeast-mediated biopurification

Cheng

Wang

et al. 2011

Microb Cell Fact

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“…Transformants were selected on yeast peptone-dextrose-agar plates with Zeocin (100 g/ml) at 28°C (11). PCR analysis of genomic DNA was used to confirm recombination of the plasmid into the genomic DNA (42). Resistant colonies were first grown to generate biomass and then grown in buffered minimal medium as described (11).…”

Section: Methodsmentioning

confidence: 99%

Catalytic Convergence of Manganese and Iron Lipoxygenases by Replacement of a Single Amino Acid

Wennman

Jernerén

Hámberg³

et al. 2012

Journal of Biological Chemistry

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Background: 13R-MnLOX catalyzes suprafacial hydrogen abstraction and oxygenation in contrast to sLOX-1. Results: Mutation of one residue of 13R-MnLOX altered hydrogen abstraction and oxygenation to antarafacial. Conclusion: Replacement with the corresponding residue of soybean LOX-1 yielded catalytic convergence. Significance: The suprafacial oxygenation mechanism can be attributed to a single amino acid substitution.

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“…They are as follows: 1. Protocol A: Method described by Cheng and Jiang [12] 2. Protocol B: Boiling Method described by Abdulla [13] 3.…”

Section: Dna Extractionmentioning

confidence: 99%

Bioremediation of heavy metals from aquatic environment through microbial processes: A potential role for probiotics?

2017

J App Biol Biotech

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Heavy metals can cause disastrous effects on any living organisms. Probiotics have the ability to reduce metal toxicity. Bioremediation of polluted waters using these bacteria could be an alternative to conventional remediation methods. The aim of this study was to isolate and characterize heavy metal resistant probiotics. Soil samples were collected for the isolation of probiotics. Morphological, biochemical, and molecular characterization was performed for the isolates. In addition, a preliminary heavy metal minimum inhibitory concentration test was done followed by atomic absorption spectrometry (AAS) analysis analysis. Four Enterococci (BT1, BT2, MC1, and MC2) and Bacillus acidiproducens (SM1 and SM2) were isolated. Moreover, all the isolates demonstrated probiotic characteristics. BT1 and BT2 were able to tolerate mercury (Hg), cadmium (Cd), lead (Pb), and chromium (Cr) but they demonstrated poor Hg removal abilities (0.75-1.42%). MC1 and MC2 isolates could grow in medium supplemented with Cd, Pb, and Cr, respectively. MC1 showed the highest level of Pb removal (43.00% ± 0.776%) and Cd removal (46.19% ± 7.651%) from broth media. Yet, SM1 and SM2 isolates tolerated only Pb and Cr. SM2 had the ability to remove the highest amount of Cr (43.06% ± 7.991%). These reasonable heavy metal removal abilities could be further studied for efficient use in bioremediation.

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Extremely Rapid Extraction of DNA from Bacteria and Yeasts

Cited by 283 publications

References 11 publications

A novel method to prepare L-Arabinose from xylose mother liquor by yeast-mediated biopurification

A novel method to prepare L-Arabinose from xylose mother liquor by yeast-mediated biopurification

Catalytic Convergence of Manganese and Iron Lipoxygenases by Replacement of a Single Amino Acid

Bioremediation of heavy metals from aquatic environment through microbial processes: A potential role for probiotics?

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