Extremophile Metal Resistance: Plasmid-Encoded Functions in Streptomyces mirabilis

Brangsch, Hanka; Höller, Marlene; Krauβe, Thomas; Waqas, Mohammed; Schroeckh, Volker; Brakhage, Axel A.; Bunk, Boyke; Spröer, Cathrin; Overmann, Jörg; Kothe, Erika

doi:10.1128/aem.00085-22

Cited by 6 publications

(3 citation statements)

References 76 publications

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“…S. mirabilis P16B-1 uses a highly nickel-specific efflux pump, NreB, in order to survive under extremely high concentrations of up to 130 mM nickel as a resistance mechanism [ 16 , 18 ]. The protein shows high similarity to known nickel efflux pumps such as Cupriavidus metallidurans CH34 NreB (see Supplementary Figure S7 ).…”

Section: Discussionmentioning

confidence: 99%

“…The isolate S. mirabilis P16B-1, with its exceedingly high resistance against specifically nickel in its environment, warrants to concentrate on the role of nickel in extracellular resistance mechanisms such as biomineralization. The contribution of plasmid-encoded functions to metal resistance has previously been shown [ 15 , 16 ]. The genome sequencing (GenBank acc.…”

Section: Introductionmentioning

confidence: 99%

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Biomineralization of Nickel Struvite Linked to Metal Resistance in Streptomyces mirabilis

2022

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Biomineral formation is a common trait and prominent for soil Actinobacteria, including the genus Streptomyces. We investigated the formation of nickel-containing biominerals in the presence of a heavy-metal-resistant Streptomyces mirabilis P16B-1. Biomineralization was found to occur both in solid and liquid media. Minerals were identified with Raman spectroscopy and TEM-EDX to be either Mg-containing struvite produced in media containing no nickel, or Ni-struvite where Ni replaces the Mg when nickel was present in sufficient concentrations in the media. The precipitation of Ni-struvite reduced the concentration of nickel available in the medium. Therefore, Ni-struvite precipitation is an efficient mechanism for tolerance to nickel. We discuss the contribution of a plasmid-encoded nickel efflux transporter in aiding biomineralization. In the elevated local concentrations of Ni surrounding the cells carrying this plasmid, more biominerals occurred supporting this point of view. The biominerals formed have been quantified, showing that the conditions of growth do influence mineralization. This control is also visible in differences observed to biosynthetically synthesized Ni-struvites, including the use of sterile-filtered culture supernatant. The use of the wildtype S. mirabilis P16B-1 and its plasmid-free derivative, as well as a metal-sensitive recipient, S. lividans, and the same transformed with the plasmid, allowed us to access genetic factors involved in this partial control of biomineral formation.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Biomineralization of Nickel Struvite Linked to Metal Resistance in Streptomyces mirabilis

2022

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“…This means that under high stress condition, biodiversity tends to be lower whereas, species diversity can increase when stress level decrease [ 55 , 56 ]. Now, we know that increased levels of stress can induce genetic mutations in all living organisms, and sometimes, the mutations and survival are considered as an alternative method to adapt, proliferate, and grow in extreme conditions, with consequent production of important metabolites with medicinal properties [ 57 , 58 , 59 ].…”

Section: Introductionmentioning

confidence: 99%

The Cytotoxic Properties of Extreme Fungi’s Bioactive Components—An Updated Metabolic and Omics Overview

Kiss

Akbari

Marchev

et al. 2023

Life

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Fungi are the most diverse living organisms on planet Earth, where their ubiquitous presence in various ecosystems offers vast potential for the research and discovery of new, naturally occurring medicinal products. Concerning human health, cancer remains one of the leading causes of mortality. While extensive research is being conducted on treatments and their efficacy in various stages of cancer, finding cytotoxic drugs that target tumor cells with no/less toxicity toward normal tissue is a significant challenge. In addition, traditional cancer treatments continue to suffer from chemical resistance. Fortunately, the cytotoxic properties of several natural products derived from various microorganisms, including fungi, are now well-established. The current review aims to extract and consolidate the findings of various scientific studies that identified fungi-derived bioactive metabolites with antitumor (anticancer) properties. The antitumor secondary metabolites identified from extremophilic and extremotolerant fungi are grouped according to their biological activity and type. It became evident that the significance of these compounds, with their medicinal properties and their potential application in cancer treatment, is tremendous. Furthermore, the utilization of omics tools, analysis, and genome mining technology to identify the novel metabolites for targeted treatments is discussed. Through this review, we tried to accentuate the invaluable importance of fungi grown in extreme environments and the necessity of innovative research in discovering naturally occurring bioactive compounds for the development of novel cancer treatments.

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Microbes in High Salt and Metal Contamination

Harpke,

Kothe

2024

Geomicrobiology: Natural and Anthropogenic Settings

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Extremophile Metal Resistance: Plasmid-Encoded Functions in Streptomyces mirabilis

Abstract: Living in extremely metal-rich environments requires specific adaptations, and often, specific metal tolerance genes are encoded on a transferable plasmid. Here, Streptomyces mirabilis P16B-1, isolated from a former mining area and able to grow with up to 130 mM NiSO 4 , was investigated.

Cited by 6 publications

References 76 publications

Biomineralization of Nickel Struvite Linked to Metal Resistance in Streptomyces mirabilis

Biomineralization of Nickel Struvite Linked to Metal Resistance in Streptomyces mirabilis

The Cytotoxic Properties of Extreme Fungi’s Bioactive Components—An Updated Metabolic and Omics Overview

Microbes in High Salt and Metal Contamination

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