Extrinsic and intrinsic regulation of blood feeding in mosquitoes

Kato, Yusuke; Sakuma, Chisako

doi:10.1016/j.cois.2024.101221

Current Opinion in Insect Science

2024

DOI: 10.1016/j.cois.2024.101221

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Extrinsic and intrinsic regulation of blood feeding in mosquitoes

Yusuke Kato,

Chisako Sakuma

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2024

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A genome-wide CRISPR screen inAnophelesmosquito cells identifies essential genes and required components of clodronate liposome function

Mameli,

Samantsidis,

Viswanatha

et al. 2024

Preprint

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Anopheles mosquitoes are the sole vector of human malaria, the most burdensome vector-borne disease worldwide. Strategies aimed at reducing mosquito populations and limiting their ability to transmit disease show the most promise for disease control. Therefore, gaining an improved understanding of mosquito biology, and specifically that of the immune response, can aid efforts to develop new approaches that limit malaria transmission. Here, we use a genome-wide CRISPR screening approach for the first time in mosquito cells to identify essential genes in Anopheles and identify genes for which knockout confers resistance to clodronate liposomes, which have been widely used in mammals and arthropods to ablate immune cells. In the essential gene screen, we identified a set of 1280 Anopheles genes that are highly enriched for genes involved in fundamental cell processes. For the clodronate liposome screen, we identified several candidate resistance factors and confirm their roles in the uptake and processing of clodronate liposomes through in vivo validation in Anopheles gambiae, providing new mechanistic detail of phagolysosome formation and clodronate liposome function. In summary, we demonstrate the application of a genome-wide CRISPR knockout platform in a major malaria vector and the identification of genes that are important for fitness and immune-related processes.

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A genome-wide CRISPR screen inAnophelesmosquito cells identifies essential genes and required components of clodronate liposome function

Mameli,

Samantsidis,

Viswanatha

et al. 2024

Preprint

View full text Add to dashboard Cite

show abstract

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Extrinsic and intrinsic regulation of blood feeding in mosquitoes

Cited by 1 publication

References 54 publications

A genome-wide CRISPR screen inAnophelesmosquito cells identifies essential genes and required components of clodronate liposome function

A genome-wide CRISPR screen inAnophelesmosquito cells identifies essential genes and required components of clodronate liposome function

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