Extrinsic tagging in iron bioavailability research: a critical review

Consaul, James Ross; Lee, Ken

doi:10.1021/jf00118a002

Cited by 41 publications

(28 citation statements)

References 17 publications

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“…In a 10-day feeding trial of ascorbic acid supplementation, Perks & Miller (40) demonstrated that ascorbic acid had no effect on the bioavailability of iron from an iron-fortified milk product. This result was in agreement with a number of human studies of similar duration, but was in direct contrast to numerous single meal studies which concluded that ascorbic acid can promote iron bioavailability (77)(78)(79)(80)(81). This suggests that single meal studies may overestimate the impact of some dietary factors on mineral absorption, and therefore may not reflect true long-term bioavailabilities.…”

Section: Application Of the Porcine Model In Nutritional Researchsupporting

confidence: 89%

The Pig as an Experimental Model for Elucidating the Mechanisms Governing Dietary Influence on Mineral Absorption

Patterson

Lei²,

Miller³

2008

Exp Biol Med (Maywood)

219

181

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Section: Application Of the Porcine Model In Nutritional Researchsupporting

confidence: 89%

The Pig as an Experimental Model for Elucidating the Mechanisms Governing Dietary Influence on Mineral Absorption

Patterson

Lei²,

Miller³

2008

Exp Biol Med (Maywood)

219

181

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“…The extrinsic 59Fe incorporated into rolls containing soybean hulls was retained similarly to the intrinsic 59Fe in soybean hulls, when fed as part of a mixed Western meal. The data suggest that extrinsic labeling, a technique which has been validated for a variety of foods (Consaul and Lee, 1983), may also be a valid technique for studying nonheme iron availability from soybean hulls when fed in a meal to humans. Complete validation of the extrinsic labeling method for soybean hulls in humans would require a double isotope study, allowing measurement of retention of both intrinsic and extrinsic labels from a single meal.…”

Section: Iron Bioavailability From Test Mealsmentioning

confidence: 99%

Availability of Soybean Hull Iron Fed to Humans in a Mixed, Western Meal

Lykken

Hunt

Nielsen

et al. 1987

Journal of Food Science

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Century soybeans were grown in a greenhouse and labeled by stem injection of iron-59. The intrinsically labled soybean hulls were incorporated into bread rolls and fed in mixed meals containing beef to six free-living female volunteers. Iron-59 retention, measured by whole body counting of these volunteers, was compared with retention from similar meals containing extrinsically labeled soybean hulls or bakery grade ferrous sulfate. Iron-59 retention averaged 23% from meals containing the intrinsically labeled soybean hulls, 18% from meals containing extrinsically labeled soybean hulls, and 22% from meals containing bakery grade ferrous sulfate. There were no statistically significant differences in retention among the various iron sources, indicating that soybean hulls were an excellent source of iron for bread enrichment.

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“…Recently, the use of extrinsic labelling technique in iron bioavailability studies has become popular owing to its simplicity and low cost over the intrinsic labelling techniques [1,2]. Moreover, extrinsic radioactive iron behaves in a manner entirely analogous to the element intrinsic to most foods.…”

Section: Introductionmentioning

confidence: 99%

Determination of iron absorption by rat bioassay. Evaluation of methods of dosing59Fe on radioiron absorption from plant diets

Buchowski

Mahoney

Kalpalathika

et al. 1991

Plant Food Hum Nutr

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We studied the potential of an animal model to predict nonheme iron absorption in humans and tested a feasible and easy technique of dosing 59Fe to evaluate bioavailability of dietary nonheme iron. Plant diets containing about 20 ppm or 75 ppm iron were the nonheme iron sources with ferrous sulfate (75 ppm) as a reference. Radioiron was administered by (a) gavaging in water, 1 h after a meal; (b) mixing with the meal; and (c) making a slurry with the meal and gavaging. No significant differences were found (P less than or equal to 0.05) in 59Fe absorption among the three methods of administering radioiron. Absorption of 59Fe was similar to apparent iron absorption for all diets tested, whether the animals consumed the same diet as that of the test meal or a different diet. A high correlation (r = 0.88) obtained between the apparent iron absorption and 59Fe iron absorption for different dosing techniques, indicates that extrinsic iron tag administered by any of these methods is valid to measure nonheme iron absorption. Apparent iron absorption values determined by rats fed 20 ppm or 75 ppm dietary iron from plant sources were similar to reported values for humans. It is concluded that the normal adult rat is an appropriate model to study iron bioavailability of human foods when iron status, maturity, iron intake relative to requirement, and method of measurement are similar to the human situation.

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Extrinsic tagging in iron bioavailability research: a critical review

Cited by 41 publications

References 17 publications

The Pig as an Experimental Model for Elucidating the Mechanisms Governing Dietary Influence on Mineral Absorption

The Pig as an Experimental Model for Elucidating the Mechanisms Governing Dietary Influence on Mineral Absorption

Availability of Soybean Hull Iron Fed to Humans in a Mixed, Western Meal

Determination of iron absorption by rat bioassay. Evaluation of methods of dosing59Fe on radioiron absorption from plant diets

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