Extrusion-Based Bioprinting through Glucose-Mediated Enzymatic Hydrogelation

Gantumur, Enkhtuul; Nakahata, Masaki; Kojima, Masaru; Sakai, Shinji

doi:10.18063/ijb.v6i1.250

Cited by 25 publications

(17 citation statements)

References 33 publications

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“…Horseradish peroxidase (HRP)-catalyzed gelation of aqueous solutions containing a gelatin derivative with phenolic hydroxyl (Ph) moieties (Gelatin-Ph) is one of the well-known methods, which has been used for obtaining gelatin-based hydrogels stable at around 37 °C. , Due to good cytocompatibility and biocompatibility, − the hydrogels have been studied for varieties of biomedical applications, such as vehicles for retinal progenitor cell transplantation, carriers of drugs for bone regeneration, cell-laden wound dressing, and inks for bioprinting. , …”

Section: Introductionmentioning

confidence: 99%

Influence of Hydrogen Peroxide-Mediated Cross-Linking and Degradation on Cell-Adhesive Gelatin Hydrogels

Mubarok

Sakai

2021

ACS Appl. Bio Mater.

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Hydrogen peroxide (H 2 O 2 ) is widely used for the gelation of aqueous solutions of gelatin derivatives with phenolic hydroxyl groups (Gelatin-Ph) catalyzed by horseradish peroxidase (HRP). Apart from this, H 2 O 2 is known to cause degradation/depolymerization of various polymers. Here, we prepared Gelatin-Ph hydrogels from solutions containing Gelatin-Ph and HRP by continuously supplying H 2 O 2 from the gas phase and investigated the mechanical properties of resultant hydrogels and the behaviors of rat fibroblast and human adipose-derived stem cells on them. Young's modulus of the hydrogel obtained from 5 w/v % Gelatin-Ph and 1 and 5 U/mL HRP increased when the exposure time to air containing H 2 O 2 (16 ppm) was extended from 15 to 30 min. However, further prolonging the exposure time to 60 min reduced Young's modulus to the same magnitude as for the hydrogels exposed to air containing H 2 O 2 for 15 min. Interestingly, the cell length and aspect ratio of the cells continued to increase, as the exposure time was extended, without reflecting the decrease in Young's modulus. These results indicate that when preparing Gelatin-Ph hydrogels through HRP/H 2 O 2 -mediated gelation, it is necessary to consider the effect of the degradation of Gelatin-Ph caused by H 2 O 2 on the mechanical properties of the resultant hydrogels and the behaviors of cells on them.

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Section: Introductionmentioning

confidence: 99%

Influence of Hydrogen Peroxide-Mediated Cross-Linking and Degradation on Cell-Adhesive Gelatin Hydrogels

Mubarok

Sakai

2021

ACS Appl. Bio Mater.

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“…The high diversity of chemical structures and properties of these plant polysaccharides opens the possibility for their application in several fields, including in the food area (e.g., as gelling agents) [ 97 ], in the biomedical field (e.g., in drug delivery systems) [ 98 ], and, more recently, in tissue engineering [ 99 ]. The interest in using some of these plant-derived polysaccharides in the formulation of bioinks for 3D bioprinting applications has also grown considerably in the later years, as summed up in Table 2 , with several works using cellulose and nanocelluloses [ 100 , 101 , 102 , 103 , 104 , 105 , 106 , 107 , 108 , 109 , 110 , 111 , 112 , 113 , 114 , 115 , 116 , 117 , 118 ] and pectin [ 119 , 120 ].…”

Section: Polysaccharide-based Hydrogel Bioinksmentioning

confidence: 99%

“…CELLINK Bioink from Cellink [ 132 ]; throughout the years, not much evolution and creativity has been observed regarding the use of NFC as a bioink component. Most recent studies still describe its combination with alginate for bioprinting of different cell lines, namely human cartilage [ 115 , 116 ], human dermis [ 117 ], bone [ 102 , 118 ], and skeletal muscle [ 103 ]. Additionally, nanocelluloses are still mainly used as reinforcing components for hydrogel bioinks due to their excellent mechanical properties.…”

Section: Polysaccharide-based Hydrogel Bioinksmentioning

confidence: 99%

A Guide to Polysaccharide-Based Hydrogel Bioinks for 3D Bioprinting Applications

Teixeira

Lameirinhas

Carvalho

et al. 2022

IJMS

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Three-dimensional (3D) bioprinting is an innovative technology in the biomedical field, allowing the fabrication of living constructs through an approach of layer-by-layer deposition of cell-laden inks, the so-called bioinks. An ideal bioink should possess proper mechanical, rheological, chemical, and biological characteristics to ensure high cell viability and the production of tissue constructs with dimensional stability and shape fidelity. Among the several types of bioinks, hydrogels are extremely appealing as they have many similarities with the extracellular matrix, providing a highly hydrated environment for cell proliferation and tunability in terms of mechanical and rheological properties. Hydrogels derived from natural polymers, and polysaccharides, in particular, are an excellent platform to mimic the extracellular matrix, given their low cytotoxicity, high hydrophilicity, and diversity of structures. In fact, polysaccharide-based hydrogels are trendy materials for 3D bioprinting since they are abundant and combine adequate physicochemical and biomimetic features for the development of novel bioinks. Thus, this review portrays the most relevant advances in polysaccharide-based hydrogel bioinks for 3D bioprinting, focusing on the last five years, with emphasis on their properties, advantages, and limitations, considering polysaccharide families classified according to their source, namely from seaweed, higher plants, microbial, and animal (particularly crustaceans) origin.

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“…It enables the development of kidney tissue sheets in a uniform pattern with increased nephron cells together with a functional proximal tubular, and thereby effectively improves the quality control, scale, and structure when compared to the manual organoid method [21]. In another study, an extrusion-based bioprinting approach utilized bio-ink consisting of living cells, glucose, alginate with a phenolic hydroxyl group, and cellulose nanofiber to develop lattice and human nose-shaped 3D constructs that remained stable for more than a week [22]. Gospodinova et al [23] reported that extrusion-based 3D printing helps in developing cervical cancer models by using hydroxyethyl cellulose-based bio-inks with different amounts of sodium alginate embedded with HeLa cells.…”

Section: Extrusion-based Printingmentioning

confidence: 99%

Three-Dimensional (3D) Printing in Cancer Therapy and Diagnostics: Current Status and Future Perspectives

Safhi

2022

Pharmaceuticals

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Three-dimensional (3D) printing is a technique where the products are printed layer-by-layer via a series of cross-sectional slices with the exact deposition of different cell types and biomaterials based on computer-aided design software. Three-dimensional printing can be divided into several approaches, such as extrusion-based printing, laser-induced forward transfer-based printing systems, and so on. Bio-ink is a crucial tool necessary for the fabrication of the 3D construct of living tissue in order to mimic the native tissue/cells using 3D printing technology. The formation of 3D software helps in the development of novel drug delivery systems with drug screening potential, as well as 3D constructs of tumor models. Additionally, several complex structures of inner tissues like stroma and channels of different sizes are printed through 3D printing techniques. Three-dimensional printing technology could also be used to develop therapy training simulators for educational purposes so that learners can practice complex surgical procedures. The fabrication of implantable medical devices using 3D printing technology with less risk of infections is receiving increased attention recently. A Cancer-on-a-chip is a microfluidic device that recreates tumor physiology and allows for a continuous supply of nutrients or therapeutic compounds. In this review, based on the recent literature, we have discussed various printing methods for 3D printing and types of bio-inks, and provided information on how 3D printing plays a crucial role in cancer management.

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Extrusion-Based Bioprinting through Glucose-Mediated Enzymatic Hydrogelation

Cited by 25 publications

References 33 publications

Influence of Hydrogen Peroxide-Mediated Cross-Linking and Degradation on Cell-Adhesive Gelatin Hydrogels

Influence of Hydrogen Peroxide-Mediated Cross-Linking and Degradation on Cell-Adhesive Gelatin Hydrogels

A Guide to Polysaccharide-Based Hydrogel Bioinks for 3D Bioprinting Applications

Three-Dimensional (3D) Printing in Cancer Therapy and Diagnostics: Current Status and Future Perspectives

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