“ Ezakiella massiliensis ” sp. nov., a new bacterial species isolated from human female genital tract

Diop, Khoudia; Raoult, Didier; Bretelle, Florence; Fénollar, Florence

doi:10.1016/j.nmni.2016.09.011

Cited by 8 publications

(4 citation statements)

References 10 publications

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“…Both Lactobacillus and Halomonas were identified as the top 20 OTUs in our classification models. Ezakiella is less reported in previous research, whereas, according to a report published in 2017, a new member of Ezakiella genus, Ezakiella massiliensis , was isolated from the vagina of a woman who had sex with another woman with bacterial vaginosis 41 . In our result, the genus Ezakiella was much higher in abundance in HRCDs (data not shown), which implicated the potential involvement in vaginal microbiota dysbiosis.…”

Section: Discussionsupporting

confidence: 43%

“…Ezakiella is less reported in previous research, whereas, according to a report published in 2017, a new member of Ezakiella genus, Ezakiella massiliensis, was isolated from the vagina of a woman who had sex with another woman with bacterial vaginosis. 41 In our result, the genus Ezakiella was much higher in abundance in HRCDs (data not shown), which implicated the potential involvement in vaginal microbiota dysbiosis. However, when using a smaller sample size to construct the model, the genus Ezakiella was not included as the top 20 OTUs.…”

Section: Diagnostic Potential Of Hrcd Based On the Vaginal Microbial ...mentioning

confidence: 43%

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HPV‐related cervical diseases: Alteration of vaginal microbiotas and promising potential for diagnosis

Rao

Fan

et al. 2022

Journal of Medical Virology

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Vaginal microbiota is closely associated with women's health, however, the correlation between HPV‐related cervical disease (HRCD) and vaginal microbiota is still obscure. In this study, patients with HRCD (n = 98) and healthy controls (n = 58) in Hangzhou were recruited, and their vaginal microbiota were collected and analyzed. The composition of the vaginal microbial community was explored, and a disease classification model was developed by random forest algorithm. The results suggested that the diversity of vaginal microbiota was significantly higher in HRCDs than that in healthy controls (p < 0.05). Firmicutes is the dominant phylum in vaginal microbiota, and Lactobacillus was identified as the most altered genus between two groups (p < 0.01). Kyoto Encyclopedia of Genes and Genomes analysis suggested the difference in vaginal microbial community functions between two groups. Furthermore, we identified 10 biomarkers as the optimal marker sets for the random forest model and found a higher probability of disease values in HRCD group in discovery cohort (p < 0.0001), with an area under the receiver operating characteristic curve reaching 89.7% (95% confidence interval: 78.3%–100%). We further validated the model in both validation and independent diagnosis cohorts, confirming its accuracy in the prediction of HRCD. In conclusion, this study revealed the community composition of vaginal microbiota in HRCDs and successfully constructed a diagnostic model for HRCD.

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Section: Discussionsupporting

confidence: 43%

Section: Diagnostic Potential Of Hrcd Based On the Vaginal Microbial ...mentioning

confidence: 43%

HPV‐related cervical diseases: Alteration of vaginal microbiotas and promising potential for diagnosis

Rao

Fan

et al. 2022

Journal of Medical Virology

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“…initiate the formation of the biofilm and become a scaffolding, to which other BV-associated anaerobes, such as Atopobium vaginae, can thereafter attach [35]. Interestingly, CT was positively associated with Ezakiella spp., a recently described anaerobic Gram-positive species that can be found in human vagina [36].…”

Section: Discussionmentioning

confidence: 99%

Vaginal and Anal Microbiome during Chlamydia trachomatis Infections

et al. 2021

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Background. Chlamydia trachomatis (CT) is the agent of the most common bacterial sexually transmitted infection worldwide, with a significant impact on women’s health. Despite the increasing number of studies about the vaginal microbiome in women with CT infections, information about the composition of the anal microbiome is still lacking. Here, we assessed the bacterial community profiles of vaginal and anal ecosystems associated or not with CT infection in a cohort of Caucasian young women. Methods. A total of 26 women, including 10 with a contemporary vaginal and ano-rectal CT infection, were enrolled. Composition of vaginal and anal microbiome was studied by 16S rRNA gene profiling. Co-occurrence networks of bacterial communities and metagenome metabolic functions were determined. Results. In case of CT infection, both vaginal and anal environments were characterized by a degree of dysbiosis. Indeed, the vaginal microbiome of CT-positive women were depleted in lactobacilli, with a significant increase in dysbiosis-associated bacteria (e.g., Sneathia, Parvimonas, Megasphaera), whereas the anal microbiota of CT-infected women was characterized by higher levels of Parvimonas and Pseudomonas and lower levels of Escherichia. Interestingly, the microbiome of anus and vagina had numerous bacterial taxa in common, reflecting a significant microbial ‘sharing’ between the two sites. In the vaginal environment, CT positively correlated with Ezakiella spp. while Gardnerella vaginalis co-occurred with several dysbiosis-related microbes, regardless of CT vaginal infection. The vaginal microbiome of CT-positive females exhibited a higher involvement of chorismate and aromatic amino acid biosynthesis, as well as an increase in mixed acid fermentation. Conclusions. These data could be useful to set up new diagnostic/prognostic tools, offering new perspectives for the control of chlamydial infections.

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“…Advancement in molecular based technology has been continually expanding the list of potential agents associated with BV, including the non-cultivable bacteriobiota that has limited the use of earlier culture based techniques [65]. For example, very recently three strains namely "Olegusella massiliensis" strain KHD7 T , "Ezakiella massiliensis" strain Marseille P2951 T and "Corynebacterium fournierii" strain Marseille P2948 T were isolated from the VMB of woman with BV [66][67][68]. All these three strains were gram-positive with strain KHD7 T and strain Marseille P2951 T being strictly anaerobic, while strain Marseille P2948 T was a facultative anaerobe, that grows very well under aerobic conditions and feeble under anaerobic conditions.…”

Section: Bacterial Vaginosis (Bv) Vmb Composition In Bvmentioning

confidence: 99%

Microbiota in vaginal health and pathogenesis of recurrent vulvovaginal infections: a critical review

Kalia

Singh

Kaur

2020

Ann Clin Microbiol Antimicrob

176

139

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Recurrent vulvovaginal infections (RVVI) has not only become an epidemiological and clinical problem but also include large social and psychological consequences. Understanding the mechanisms of both commensalism and pathogenesis are necessary for the development of efficient diagnosis and treatment strategies for these enigmatic vaginal infections. Through this review, an attempt has been made to analyze vaginal microbiota (VMB) from scratch and to provide an update on its current understanding in relation to health and common RVVI i.e. bacterial vaginosis, vulvovaginal candidiaisis and Trichomoniasis, making the present review first of its kind. For this, potentially relevant studies were retrieved from data sources and critical analysis of the literature was made. Though, culture-independent methods have greatly unfolded the mystery regarding vaginal bacterial microbiome, there are only a few studies regarding the composition and diversity of vaginal mycobiome and different Trichomonas vaginalis strains. This scenario suggests a need of further studies based on comparative genomics of RVVI pathogens to improve our perceptive of RVVI pathogenesis that is still not clear (Fig. 5). Besides this, the review details the rationale for Lactobacilli dominance and changes that occur in healthy VMB throughout a women's life. Moreover, the list of possible agents continues to expand and new species recognised in both health and VVI are updated in this review. The review concludes with the controversies challenging the widely accepted dogma i.e. "VMB dominated with Lactobacilli is healthier than a diverse VMB". These controversies, over the past decade, have complicated the definition of vaginal health and vaginal infections with no definite conclusion. Thus, further studies on newly recognised microbial agents may reveal answers to these controversies. Conversely, VMB of women could be an answer but it is not enough to just look at the microbiology. We have to look at the woman itself, as VMB which is fine for one woman may be troublesome for others. These differences in women's response to the same VMB may be determined by a permutation of behavioural, cultural, genetic and various other anonymous factors, exploration of which may lead to proper definition of vaginal health and disease.

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“ Ezakiella massiliensis ” sp. nov., a new bacterial species isolated from human female genital tract

Cited by 8 publications

References 10 publications

HPV‐related cervical diseases: Alteration of vaginal microbiotas and promising potential for diagnosis

HPV‐related cervical diseases: Alteration of vaginal microbiotas and promising potential for diagnosis

Vaginal and Anal Microbiome during Chlamydia trachomatis Infections

Microbiota in vaginal health and pathogenesis of recurrent vulvovaginal infections: a critical review

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