F-actin contractile rings in protoplasts of the yeast

Jochová, Jana; Rupeš, Ivan; Streiblová, Eva

doi:10.1016/0309-1651(91)90007-6

Cited by 59 publications

(51 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, during and after assembly, this ring must be spatially maintained for a proper cell division. Several reports have described findings indicating that the AMR slides sideways in round cells deprived of a cell wall, suggesting that, beyond the cell geometry, new cleavage furrow membranes or septum ingression might play a role in stabilizing and maintaining the ring in the cell middle (84)(85)(86). Something similar was previously described for cps1-191 mutant cells depleted of either microtubules or Mid1 in the absence of septum deposition (87,88).…”

Section: Anchorage and Maintenance Of The Amr In The Cell Middle Plassupporting

confidence: 59%

The Cell Biology of Fission Yeast Septation

Cortés

Ramos

Osumi

et al. 2016

Microbiol Mol Biol Rev

View full text Add to dashboard Cite

SUMMARYIn animal cells, cytokinesis requires the formation of a cleavage furrow that divides the cell into two daughter cells. Furrow formation is achieved by constriction of an actomyosin ring that invaginates the plasma membrane. However, fungal cells contain a rigid extracellular cell wall surrounding the plasma membrane; thus, fungal cytokinesis also requires the formation of a special septum wall structure between the dividing cells. The septum biosynthesis must be strictly coordinated with the deposition of new plasma membrane material and actomyosin ring closure and must occur in such a way that no breach in the cell wall occurs at any time. Because of the high turgor pressure in the fungal cell, even a minor local defect might lead to cell lysis and death. Here we review our knowledge of the septum structure in the fission yeastSchizosaccharomyces pombeand of the recent advances in our understanding of the relationship between septum biosynthesis and actomyosin ring constriction and how the two collaborate to build a cross-walled septum able to support the high turgor pressure of the cell. In addition, we discuss the importance of the septum biosynthesis for the steady ingression of the cleavage furrow.

show abstract

Section: Anchorage and Maintenance Of The Amr In The Cell Middle Plassupporting

confidence: 59%

The Cell Biology of Fission Yeast Septation

Cortés

Ramos

Osumi

et al. 2016

Microbiol Mol Biol Rev

View full text Add to dashboard Cite

show abstract

“…Studies with S. pombe suggest that the actomyosin ring requires septum formation for its ability to constrict (107,145). One explanation for the difference between yeast and animal cells is that in yeast, the contractile force of the ring may not be sufficient to counteract the turgor pressure of the cell without cell wall formation.…”

Section: Finishing Cytokinesis In Yeast and Animal Cellsmentioning

confidence: 99%

Cytokinesis in Eukaryotes

Guertin

Trautmann

McCollum

2002

Microbiol Mol Biol Rev

250

224

View full text Add to dashboard Cite

Cytokinesis is the final event of the cell division cycle, and its completion results in irreversible partition of a mother cell into two daughter cells. Cytokinesis was one of the first cell cycle events observed by simple cell biological techniques; however, molecular characterization of cytokinesis has been slowed by its particular resistance to in vitro biochemical approaches. In recent years, the use of genetic model organisms has greatly advanced our molecular understanding of cytokinesis. While the outcome of cytokinesis is conserved in all dividing organisms, the mechanism of division varies across the major eukaryotic kingdoms. Yeasts and animals, for instance, use a contractile ring that ingresses to the cell middle in order to divide, while plant cells build new cell wall outward to the cortex. As would be expected, there is considerable conservation of molecules involved in cytokinesis between yeast and animal cells, while at first glance, plant cells seem quite different. However, in recent years, it has become clear that some aspects of division are conserved between plant, yeast, and animal cells. In this review we discuss the major recent advances in defining cytokinesis, focusing on deciding where to divide, building the division apparatus, and dividing. In addition, we discuss the complex problem of coordinating the division cycle with the nuclear cycle, which has recently become an area of intense research. In conclusion, we discuss how certain cells have utilized cytokinesis to direct development

show abstract

“…It has been proposed that activation of the signaling cascade referred to as the septation initiation network (SIN) triggers actomyosin ring constriction upon nuclear division (for review see Krapp et al, 2004). Concomitantly with ring constriction, membrane material is inserted at the division site and the septum is synthesized (Jochova et al, 1991). Centripetal septum deposition and ring constriction seem to be tightly linked.…”

Section: Introductionmentioning

confidence: 99%

The Actomyosin Ring Recruits Early Secretory Compartments to the Division Site in Fission Yeast

Vještica

Tang

Oliferenko

2008

MBoC

View full text Add to dashboard Cite

The ultimate goal of cytokinesis is to establish a membrane barrier between daughter cells. The fission yeast Schizosaccharomyces pombe utilizes an actomyosin-based division ring that is thought to provide physical force for the plasma membrane invagination. Ring constriction occurs concomitantly with the assembly of a division septum that is eventually cleaved. Membrane trafficking events such as targeting of secretory vesicles to the division site require a functional actomyosin ring suggesting that it serves as a spatial landmark. However, the extent of polarization of the secretion apparatus to the division site is presently unknown. We performed a survey of dynamics of several fluorophore-tagged proteins that served as markers for various compartments of the secretory pathway. These included markers for the endoplasmic reticulum, the COPII sites, and the early and late Golgi. The secretion machinery exhibited a marked polarization to the division site. Specifically, we observed an enrichment of the transitional endoplasmic reticulum (tER) accompanied by Golgi cisternae biogenesis. These processes required actomyosin ring assembly and the function of the EFC-domain protein Cdc15p. Cdc15p overexpression was sufficient to induce tER polarization in interphase. Thus, fission yeast polarizes its entire secretory machinery to the cell division site by utilizing molecular cues provided by the actomyosin ring. INTRODUCTIONCell division is the final event in the cell cycle that results in physical separation of two daughter cells. Despite being studied for more than a hundred years, the underlying molecular mechanisms and the cytological details of the process are still emerging. Various organisms and cell types have established multiple pathways to conduct cell division that are regulated at both signaling and structural levels (for review see Balasubramanian et al., 2004). In recent years, the fission yeast Schizosaccharomyces pombe has emerged as an attractive model for the study of cytokinesis because of its fully sequenced genome (Wood et al., 2002), a cell size convenient for cytological studies and the availability of a large set of conditional mutants compromised in various aspects of cell division (Chang et al., 1996;Balasubramanian et al., 1998).On entry into mitosis, S. pombe assembles an actomyosin ring that is thought to drive a binary cell fission through constriction, similar to many other eukaryotes, including nematodes, insects, and vertebrates (for review see Hales et al., 1999). During early mitosis, actin is assembled into a ring structure through the action of several actin nucleating and bundling proteins and molecular motors. These include the formin Cdc12p (Chang et al., 1997), profilin Cdc3p (Balasubramanian et al., 1992), the extended Fer/CIP4 (EFC) domain protein Cdc15p (Fankhauser et al., 1995), and the myosin heavy chain Myo2p (Kitayama et al., 1997) together with its light chains Cdc4p (McCollum et al., 1995;Naqvi et al., 1999) and Rlc1p (Le Goff et al., 2000). It has been proposed tha...

show abstract

F-actin contractile rings in protoplasts of the yeast

Cited by 59 publications

References 8 publications

The Cell Biology of Fission Yeast Septation

The Cell Biology of Fission Yeast Septation

Cytokinesis in Eukaryotes

The Actomyosin Ring Recruits Early Secretory Compartments to the Division Site in Fission Yeast

Contact Info

Product

Resources

About