F-box protein AFB4 plays a crucial role in plant growth, development and innate immunity

Hu, Zhubing; Keçeli, Mehmet Ali; Piisilä, Maria; Li, JingF; Survila, Mantas; Heino, Pekka; Brader, Günter; Palva, E. Tapio; Li, Jing

doi:10.1038/cr.2012.12

Cited by 26 publications

(21 citation statements)

References 11 publications

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“…Another F-box protein, AFB4, plays a crucial role in plant growth and development, with afb4 mutants showing a pleiotropic phenotype, such as strongly reduced primary root growth, fewer lateral roots, very short hypocotyls, increased leaf thickness, and noticeably delayed flowering time. 35 These data confirm the importance of the F-box protein as a developmental regulator and indicate its stability as an essential factor in regulation of SCF complex activity. The SCF complex is one of the well-studied members of the ubiquitin protein ligase E3 family, and regulates many processes, including the cell cycle, floral development, the circadian clock, and responses to plant growth regulators.…”

supporting

confidence: 70%

Cyclin-like F-box protein plays a role in growth and development of the three model species Medicago truncatula, Lotus japonicus, and Arabidopsis thaliana

Boycheva¹,

Vassileva²,

Revalska³

et al. 2015

RRB

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Abstract:In eukaryotes, F-box proteins are one of the main components of the SCF complex that belongs to the family of ubiquitin E3 ligases, which catalyze protein ubiquitination and maintain the balance between protein synthesis and degradation. In the present study, we clarified the role and function of the gene encoding cyclin-like F-box protein from Medicago truncatula using transgenic plants of the model species M. truncatula, Lotus japonicas, and Arabidopsis thaliana generated by Agrobacterium-mediated transformation. Morphological and transcriptional analyses combined with flow cytometry and histochemistry demonstrated the participation of this protein in many aspects of plant growth and development, including processes of indirect somatic embryogenesis and symbiotic nodulation. The cyclin-like F-box gene showed expression in all plant organs and tissues comprised of actively dividing cells. The observed variations in root and hypocotyl growth, leaf and silique development, ploidy levels, and leaf parameters in the obtained transgenic lines demonstrated the effects of this gene on organ development. Furthermore, knockdown of cyclin-like F-box led to accumulation of higher levels of the G2/M transition-specific gene cyclin B1:1 (CYCB1:1), suggesting its possible role in cell cycle control. Together, the collected data suggest a similar role of the cyclin-like F-box protein in the three model species, providing evidence for the functional conservation of the studied gene.

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supporting

confidence: 70%

Cyclin-like F-box protein plays a role in growth and development of the three model species Medicago truncatula, Lotus japonicus, and Arabidopsis thaliana

Boycheva¹,

Vassileva²,

Revalska³

et al. 2015

RRB

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“…The AFB family provides a test case for genome evolution after gene duplication, as there is evidence of both significant novelty and redundancy between family members (Dharmasiri et al 2005a;Walsh et al 2006;Parry et al 2009;Hu et al 2012). Analysis of intraspecific coding sequence polymorphisms in this study has identified a subset of the tolerated polymorphisms Figure 4 tir1-1 is a dominant negative allele.…”

Section: Discussionmentioning

confidence: 83%

Insights into the Evolution and Function of Auxin Signaling F-Box Proteins in Arabidopsis thaliana Through Synthetic Analysis of Natural Variants

et al. 2017

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The evolution of complex body plans in land plants has been paralleled by gene duplication and divergence within nuclear auxin-signaling networks. A deep mechanistic understanding of auxin signaling proteins therefore may allow rational engineering of novel plant architectures. Toward that end, we analyzed natural variation in the auxin receptor F-box family of wild accessions of the reference plant and used this information to populate a structure/function map. We employed a synthetic assay to identify natural hypermorphic F-box variants and then assayed auxin-associated phenotypes in accessions expressing these variants. To more directly measure the impact of the strongest variant in our synthetic assay on auxin sensitivity, we generated transgenic plants expressing this allele. Together, our findings link evolved sequence variation to altered molecular performance and auxin sensitivity. This approach demonstrates the potential for combining synthetic biology approaches with quantitative phenotypes to harness the wealth of available sequence information and guide future engineering efforts of diverse signaling pathways.

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“…The 272 HpLRR transcriptional genes underwent a BLAST analysis (Supplemental Material 1) involving six protein databases (nr/nt, SwissProt, KEGG, COG, InterPro, and GO). Based on the BLAST analysis, these genes were annotated as belonging to the LRR-STK subfamily (135), FBXL subfamily (49), NBS-LRR subfamily (29), LRR-RLK subfamily (26), plant intracellular Ras group-related LRR (PIRL) subfamily (8), LRR transmembrane protein kinase subfamily (5), brassinosteroid LRR receptor kinase subfamily (3), and other LRR genes (17) ( Table 1). Heatmaps of the 272 HpLRR gene expression levels in D1, D3, N2 and N3 samples are presented in Figs.…”

Section: Resultsmentioning

confidence: 99%

“…Genome-wide analyses showed that F-box genes respond to salt stress, heavy metals, and drought in soybean and the legume Medicago truncatula [27,28]. Auxin-signaling F-box protein 4 (AFB4), which is similar to the auxin receptor known as Toll/ interleukin-1 receptor (TIR1), plays a pivotal role in plant growth, development, and innate immunity, as shown by a combination of physiological, molecular, and genetic approaches [29]. An interesting study by Angot et.al.…”

Section: Introductionmentioning

confidence: 99%

Molecular characterization and expression analysis of pitaya (Hylocereus polyrhizus) HpLRR genes in response to Neoscytalidium dimidiatum infection

Liu

et al. 2020

BMC Plant Biol

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Background: Canker disease caused by Neoscytalidium dimidiatum is a devastating disease resulting in a major loss to the pitaya industry. However, resistance proteins in plants play crucial roles to against pathogen infection. Among resistance proteins, the leucine-rich repeat (LRR) protein is a major family that plays crucial roles in plant growth, development, and biotic and abiotic stress responses, especially in disease defense. Results: In the present study, a transcriptomics analysis identified a total of 272 LRR genes, 233 of which had coding sequences (CDSs), in the plant pitaya (Hylocereus polyrhizus) in response to fungal Neoscytalidium dimidiatum infection. These genes were divided into various subgroups based on specific domains and phylogenetic analysis. Molecular characterization, functional annotation of proteins, and an expression analysis of the LRR genes were conducted. Additionally, four LRR genes (CL445.Contig4_All, Unigene28_All, CL28.Contig2_All, and Unigene2712_All, which were selected because they had the four longest CDSs were further assessed using quantitative reverse transcription PCR (qRT-PCR) at different fungal infection stages in different pitaya species (Hylocereus polyrhizus and Hylocereus undatus), in different pitaya tissues, and after treatment with salicylic acid (SA), methyl jasmonate (MeJA), and abscisic acid (ABA) hormones. The associated protein functions and roles in signaling pathways were identified. Conclusions: This study provides a comprehensive overview of the HpLRR family genes at transcriptional level in pitaya in response to N. dimidiatum infection, it will be helpful to understand the molecular mechanism of pitaya canker disease, and lay a strong foundation for further research.

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F-box protein AFB4 plays a crucial role in plant growth, development and innate immunity

Cited by 26 publications

References 11 publications

Cyclin-like F-box protein plays a role in growth and development of the three model species Medicago truncatula, Lotus japonicus, and Arabidopsis thaliana

Cyclin-like F-box protein plays a role in growth and development of the three model species Medicago truncatula, Lotus japonicus, and Arabidopsis thaliana

Insights into the Evolution and Function of Auxin Signaling F-Box Proteins in Arabidopsis thaliana Through Synthetic Analysis of Natural Variants

Molecular characterization and expression analysis of pitaya (Hylocereus polyrhizus) HpLRR genes in response to Neoscytalidium dimidiatum infection

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