F052 Effect of dietary fish oil on plasma lipoprotein cholesterol in rats fed a diet enriched in cholesterol and sucrose

Chiang, Meng-Tsan; Tsai, Ming‐Hsien

doi:10.1016/s0021-9150(97)84624-2

Cited by 14 publications

(18 citation statements)

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“…cholesterol-enriched diets) both species displayed an increase in total serum cholesterol but each presented a very different lipoprotein cholesterol profile. Thus, rats fed cholesterol or atherogenic diets develop hypercholesterolaemia that is defined by the presence of low HDL-C levels, a moderate increase in LDL-C, and very high levels of VLDL-C [30][31][32][33], while dietary cholesterol raises LDL-C and HDL-C levels in humans [34].…”

Section: Introductionmentioning

confidence: 99%

Do not use the Friedewald formula to calculate LDL‐cholesterol in hypercholesterolaemic rats

Sánchez‐Muniz

Bastida²

2008

Euro J Lipid Sci & Tech

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Reputable calculations such as the Friedewald formula are used extensively to determine LDL‐cholesterol (LDL‐C) values from known total cholesterol, triacylglycerol and HDL‐cholesterol (HDL‐C) levels. To the best of our knowledge, however, the validity of this equation has not yet been confirmed in rats. The aim of the present study is to give some insights as to why this formula must be used carefully in rats, and to find cut‐off points below which this formula can be considered reliable. Sera of 54 rats with different cholesterol, triacylglycerol and HDL‐C levels were tested. LDL was isolated by ultracentrifugation and LDL‐C measured by an enzymatic colorimetric method and compared against LDL‐C obtained by the formula. In rats whose serum cholesterol was <100 mg/dL, or whose HDL‐C constituted ≥75% of total cholesterol, or whose cholesterol/phospholipids ratio was <1, or whose serum did not contain β‐VLDL, LDL‐C obtained by both methods did not significantly differ. Under other conditions, however, and particularly in hypercholesterolaemic rats who did present β‐VLDL, the results clearly show that the Friedewald formula overestimates LDL‐C levels. In conclusion, (VLDL + LDL)‐C instead of VLDL‐C and LDL‐C must be used when ultracentrifugation or other alternative methods are not available to measure LDL‐C in hypercholesterolaemic rats.

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Section: Introductionmentioning

confidence: 99%

Do not use the Friedewald formula to calculate LDL‐cholesterol in hypercholesterolaemic rats

Sánchez‐Muniz

Bastida²

2008

Euro J Lipid Sci & Tech

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“…Liu et al (1995) showed that the addition of Cho to diet promotes TG synthesis and lipid accumulation in the liver. Chiang et al (1998) also reported that the dietary addition of Cho and cholate increased Cho levels in the blood and liver. Since Cho and cholate-added diets were used in this study, TG and Cho liver accumulation was thought to be promoted.…”

Section: Discussionmentioning

confidence: 91%

Improving Effects of Narazuke Lees on Fatty Liver of Rats Induced by High-Fat and High-Cholesterol Diets

Nakasa

Yamagami

Tanaka³

et al. 2014

FSTR

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With the purpose of using Narazuke lees as a functional foods material, its effect on fatty liver of rats fed a high-fat and high-cholesterol diet was investigated. While significant accumulation of triacylglycerol was observed in the liver of rats fed high-fat and high-cholesterol diet was observed, rats fed diet supplemented with Narazuke lees showed significantly increased fecal dry weight, as well as increased fecal excretion of total lipids and total bile acids. Further, free fatty acids, glucose and insulin concentrations in the blood were significantly decreased, and amelioration of insulin resistance was observed. In the liver, reduction of FAS activity, significant increase in CPTIa activity and promotion of fatty acid β-oxidation were observed. An improving effect on fatty liver was observed by the suppression of lipid accumulation. The amount of liver TBARS was significantly reduced, indicating the inhibition of NASH progression.

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“…It may be noteworthy that the amounts of both cholesterol and sodium cholate used to supplement the diets in this study were smaller than other reported high cholesterol diets fed in order to induce experimental hypercholesterolemia. [6][7][8][9][10][11]19,20) …”

Section: Discussionmentioning

confidence: 99%

“…[3][4][5][6][7][8] In this study, we also used high cholesterol diets supplemented with cholic acid, and we observed changes in erythrocyte lipids, including a decrease in cholesterol and changes in the fatty acid composition of phospholipids (PL). Dietary fatty acids may induce extensive modiˆcation in the fatty acid composition of cell membranes, including erythrocytes.…”

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confidence: 99%

High-cholesterol Diets Induce Changes in Lipid Composition of Rat Erythrocyte Membrane Including Decrease in Cholesterol, Increase in α-Tocopherol and Changes in Fatty Acids of Phospholipids

Shiro

Ohnishi

Kaji

et al. 2003

Bioscience, Biotechnology, and Biochemistry

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F052 Effect of dietary fish oil on plasma lipoprotein cholesterol in rats fed a diet enriched in cholesterol and sucrose

Cited by 14 publications

References 0 publications

Do not use the Friedewald formula to calculate LDL‐cholesterol in hypercholesterolaemic rats

Do not use the Friedewald formula to calculate LDL‐cholesterol in hypercholesterolaemic rats

Improving Effects of Narazuke Lees on Fatty Liver of Rats Induced by High-Fat and High-Cholesterol Diets

High-cholesterol Diets Induce Changes in Lipid Composition of Rat Erythrocyte Membrane Including Decrease in Cholesterol, Increase in α-Tocopherol and Changes in Fatty Acids of Phospholipids

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