Fabricating an Acetylcholinesterase Modulated UCNPs-Cu²⁺ Fluorescence Biosensor for Ultrasensitive Detection of Organophosphorus Pesticides-Diazinon in Food

Abstract

In this study, a highly sensitive upconversion fluorescence (FL) biosensor was developed for the detection of organophosphorus pesticides (OPs) based on an acetylcholinesterase (AChE) modulated FL "off−on−off" strategy. The luminescence of synthesized UCNPs could be quenched strongly by Cu 2+ due to an energy transfer effect. Upon addition of AChE and acetylthiocholine (ATCh), the enzymatic hydrolysate (thiocholine) could seize Cu 2+ from UCNPs-Cu 2+ mixture, resulting in the quenched FL triggered on. OPs could irreversibly impede the activity of AChE, which caused the formation of thiocholine to decrease, thus, reduced the recovery of FL. Under the optimum conditions, a linear detection range from 0.1 to 50 ng/mL was achieved for the representative OPs (diazinon) with LOD of 0.05 ng/mL. Furthermore, the ability of the biosensor to detect OPs was also confirmed in adulterated environmental and agricultural samples. In validation analysis, the proposed sensor showed satisfactory results (p > 0.05) with GC−MS.

Modulation impact of Diazinon forms on gene expression profile and DNA damage pathway in male mice

“…Exposure of DZN to the surrounding environment such as U.V. radiation, elevated heat, moisture, and air results in many degradable product formations such as tetraethyl dithiopyrophosphate (S, S-TEPP, and sulfo-TEPP) and tetraethylmonothiopyrophosphate (O, S-TEPP, and mono-TEPP) (Wang et al, 2019). Furthermore, metal ions from corroded metal containers act as catalytic agents to decompose DZN to these toxic products (Zhang et al, 2019).…”

What are the Main Sensor Methods for Quantifying Pesticides in Agricultural Activities? A Review

“…It was found that for diazinon (DZ) the lowest LOD was 5.00 × 10 −4 μg/mL using an AChE biosensor based on the modulated fluorescence [121] of UCNPs-Cu 2+ , in contrast to the method of MFEM-MS [42], which registered a value of 4.00 × 10 −03 μg/mL. In the case of the herbicide DU, 6.99 × 10 −6 μg/mL was the lowest value of LOD, obtained by means of the square wave voltammetry technique [52], whereas by the DPX-HPLC/DAD method [23], a value of 6.00 × 10 −5 μg/mL was obtained, this being the only case where a similar LOD value was obtained for the aqueous matrices.…”

Recent Advances on Detection of Insecticides Using Optical Sensors

“…As a result of an energy transfer effect, the luminescence of synthesized UCNPs could be strongly quenched by Cu 2+ . The enzymatic hydrolyzate (thiocholine) could seize Cu 2+ from the UCNPs-Cu 2+ mixture after the addition of AChE and ATCh, resulting in the quenched fluorescence being activated [ 197 ].…”