Fabrication and Application of a 3D‐Printed Poly‐<i>ε</i>‐Caprolactone Cage Scaffold for Bone Tissue Engineering

Wang, Siyi; Li, Rong; Xu, Yongxiang; Xia, Dandan; Zhu, Yuan; Yoon, Jungmin; Gu, Ranli; Liu, Xuenan; Zhao, Wenyan; Zhao, Xin; Liu, Yunsong; Sun, Yuchun; Zhou, Yongsheng

doi:10.1155/2020/2087475

Cited by 18 publications

(29 citation statements)

References 32 publications

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“…Bone defects caused by various conditions, such as congenital malformation, trauma, tumor resection, and infection, not only cause great pain but also place tremendous pressure on healthcare systems [ 1 ]. Bone tissue engineering (BTE) emerging in recent decades now provides an option for the therapy of bone defects.…”

Section: Introductionmentioning

confidence: 99%

The impact of Zn-doped synthetic polymer materials on bone regeneration: a systematic review

Wang

Xia

et al. 2021

Stem Cell Res Ther

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Introduction To repair bone defects, a variety of bone substitution materials have been used, such as ceramics, metals, natural and synthetic polymers, and combinations thereof. In recent decades, a wide range of synthetic polymers have been used for bone regeneration. These polymers have the advantages of biocompatibility, biodegradability, good mechanical properties, low toxicity, and ease of processing. However, when used alone, they are unable to achieve ideal bone formation. Incorporating zinc (Zn) into synthetic polymers has been considered, as previous studies have shown that Zn2+ promotes stem cell osteogenesis and mineral deposition. The purpose of this systematic review was to provide an overview of the application and effectiveness of Zn in synthetic polymers for bone regeneration, whether used alone or in combination with other biomaterials. This study was performed according to the PRISMA guidelines. Materials and methods A search of the PubMed, Embase, and the Cochrane Library databases for articles published up to June 2020 revealed 153 relevant studies. After screening the titles, abstracts, and full texts, 13 articles were included in the review; 9 of these were in vitro, 3 were in vivo, and 1 included both in vitro and in vivo experiments. Results At low concentrations, Zn2+ promoted cell proliferation and osteogenic differentiation, while high-dose Zn2+ resulted in cytotoxicity and inhibition of osteogenic differentiation. Additionally, one study showed that Zn2+ reduced apatite formation in simulated body fluid. In all of the in vivo experiments, Zn-containing materials enhanced bone formation. Conclusions At appropriate concentrations, Zn-doped synthetic polymer materials are better able to promote bone regeneration than materials without Zn.

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Section: Introductionmentioning

confidence: 99%

The impact of Zn-doped synthetic polymer materials on bone regeneration: a systematic review

Wang

Xia

et al. 2021

Stem Cell Res Ther

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“…The surface affinity of a scaffold with water and proteins influences biological responses to the scaffold. [39][40][41] Thus, water absorption and protein adhesion tests were carried out as part of the present study. In the water absorption test, Ap-CS absorbed more water than did CS (Figure 4A), suggesting that the apatite coating on Ap-CS increased the surface hydrophilicity of the scaffold.…”

Section: Discussionmentioning

confidence: 99%

Periodontal tissue engineering using an apatite/collagen scaffold obtained by a plasma‐ and precursor‐assisted biomimetic process

Kanemoto

Miyaji

Nishida

et al. 2021

J of Periodontal Research

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Background and objectives:In the treatment of severe periodontal destruction, there is a strong demand for advanced scaffolds that can regenerate periodontal tissues with adequate quality and quantity. Recently, we developed a plasma-and precursorassisted biomimetic process by which a porous collagen scaffold (CS) could be coated with low-crystalline apatite. The apatite-coated collagen scaffold (Ap-CS) promotes cellular ingrowth within the scaffold compared to CS in rat subcutaneous tissue. In the present study, the osteogenic activity of Ap-CS was characterized by cell culture and rat skull augmentation tests. In addition, the periodontal tissue reconstruction with Ap-CS in a beagle dog was compared to that with CS. Methods:The plasma-and precursor-assisted biomimetic process was applied to CS to obtain Ap-CS with a low-crystalline apatite coating. The effects of apatite coating on the scaffold characteristics (i.e., surface morphology, water absorption, Ca release, protein adsorption, and enzymatic degradation resistance) were assessed.Cyto-compatibility and the osteogenic properties of Ap-CS and CS were assessed in vitro using preosteoblastic MC3T3-E1 cells. In addition, we performed in vivo studies to evaluate bone augmentation and periodontal tissue reconstruction with Ap-CS and CS in a rat skull and canine furcation lesion, respectively.Results: As previously reported, the plasma-and precursor-assisted biomimetic process generated a low-crystalline apatite layer with a nanoporous structure that uniformly covered the Ap-CS surface. Ap-CS showed significantly higher water absorption, Ca release, lysozyme adsorption, and collagenase resistance than CS. Cell culture experiments revealed that Ap-CS was superior to CS in promoting the osteoblastic differentiation of MC3T3-E1 cells while suppressing their proliferation.Additionally, Ap-CS significantly promoted (compared to CS) the augmentation of the rat skull bone and showed the potential to regenerate alveolar bone in a dog furcation defect.How to cite this article: Kanemoto Y, Miyaji H, Nishida E, et al.Periodontal tissue engineering using an apatite/collagen scaffold obtained by a plasma-and precursor-assisted biomimetic process.

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“…Therefore, the BMSCs–scaffold construct was cultured in vitro for 21 days before implantation, which makes it more conducive to the synthesis and accumulation of cartilage ECM, resulting in more mature structure and function, so that it responds more quickly to knee joint pressure after implantation. In addition, studies showed that surface modification of the PCL scaffold increases the adhesion, proliferation, and differentiation characteristics of seeded cells, which is an exploration direction for us to further optimize and modify the meniscus scaffold [ 31 , 32 ].…”

Section: Discussionmentioning

confidence: 99%

Parathyroid hormone (1-34) promotes the effects of 3D printed scaffold-seeded bone marrow mesenchymal stem cells on meniscus regeneration

et al. 2020

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Background Cell-based tissue engineering represents a promising management for meniscus repair and regeneration. The present study aimed to investigate whether the injection of parathyroid hormone (PTH) (1-34) could promote the regeneration and chondroprotection of 3D printed scaffold seeded with bone marrow mesenchymal stem cells (BMSCs) in a canine total meniscal meniscectomy model. Methods 3D printed poly(e-caprolactone) scaffold seeded with BMSCs was cultured in vitro, and the effects of in vitro culture time on cell growth and matrix synthesis of the BMSCs–scaffold construct were evaluated by microscopic observation and cartilage matrix content detection at 7, 14, 21, and 28 days. After that, the tissue-engineered meniscus based on BMSCs–scaffold cultured for the appropriate culture time was selected for in vivo implantation. Sixteen dogs were randomly divided into four groups: PTH + BMSCs–scaffold, BMSCs–scaffold, total meniscectomy, and sham operation. The regeneration of the implanted tissue and the degeneration of articular cartilage were assessed by gross, histological, and immunohistochemical analysis at 12 weeks postoperatively. Results In vitro study showed that the glycosaminoglycan (GAG)/DNA ratio and the expression of collagen type II (Col2) were significantly higher on day 21 as compared to the other time points. In vivo study showed that, compared with the BMSCs–scaffold group, the PTH + BMSCs–scaffold group showed better regeneration of the implanted tissue and greater similarity to native meniscus concerning gross appearance, cell composition, and cartilage extracellular matrix deposition. This group also showed less expression of terminal differentiation markers of BMSC chondrogenesis as well as lower cartilage degeneration with less damage on the knee cartilage surface, higher expression of Col2, and lower expression of degeneration markers. Conclusions Our results demonstrated that PTH (1-34) promotes the regenerative and chondroprotective effects of the BMSCs–3D printed meniscal scaffold in a canine model, and thus, their combination could be a promising strategy for meniscus tissue engineering.

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Fabrication and Application of a 3D‐Printed Poly‐ε‐Caprolactone Cage Scaffold for Bone Tissue Engineering

Cited by 18 publications

References 32 publications

The impact of Zn-doped synthetic polymer materials on bone regeneration: a systematic review

The impact of Zn-doped synthetic polymer materials on bone regeneration: a systematic review

Periodontal tissue engineering using an apatite/collagen scaffold obtained by a plasma‐ and precursor‐assisted biomimetic process

Parathyroid hormone (1-34) promotes the effects of 3D printed scaffold-seeded bone marrow mesenchymal stem cells on meniscus regeneration

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