Fabrication and characterization of a chemically oxidized-nanostructured porous silicon based biosensor implementing orienting protein A

Naveas, Nelson; Hernández‐Montelongo, Jacobo; Pulido, Ruth; Torres-Costa, V.; Villanueva-Guerrero, Raúl; Ruiz, Josefa Predestinación García; Manso‐Silván, Miguel

doi:10.1016/j.colsurfb.2013.11.026

Cited by 18 publications

(11 citation statements)

References 23 publications

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“…However, they suffer from limited sensitivity and risk of loss of specificity. To improve the performances, porous silicon (PSi) is a good candidate to replace the conventional substrates in novel chemical sensors and biosensors [17]. The high specific surface area of PSi together with the control of the physico-chemical behavior of its nanostructured surface, make of this material a versatile substrate for the development of sensors.…”

Section: Introductionmentioning

confidence: 99%

Gold nanoparticle triggered dual optoplasmonic-impedimetric sensing of prostate-specific antigen on interdigitated porous silicon platforms

Rodriguez

Torres-Costa

Ahumada³

et al. 2018

Sensors and Actuators B: Chemical

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Section: Introductionmentioning

confidence: 99%

Gold nanoparticle triggered dual optoplasmonic-impedimetric sensing of prostate-specific antigen on interdigitated porous silicon platforms

Rodriguez

Torres-Costa

Ahumada³

et al. 2018

Sensors and Actuators B: Chemical

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“…2 eV and the C=O at BE 288.5 eV. The first two contributions of the C1s spectrum (right) confirm the high level of integration of the aminopropyl group, while the N1s spectrum in the inset shows that the primary amines are present in neutral and charged forms [33,34] for the low and high BE contributions, respectively.…”

Section: Resultsmentioning

confidence: 90%

Visible Light Assisted Organosilane Assembly on Mesoporous Silicon Films and Particles

et al. 2019

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Porous silicon (PSi) is a versatile matrix with tailorable surface reactivity, which allows the processing of a range of multifunctional films and particles. The biomedical applications of PSi often require a surface capping with organic functionalities. This work shows that visible light can be used to catalyze the assembly of organosilanes on the PSi, as demonstrated with two organosilanes: aminopropyl-triethoxy-silane and perfluorodecyl-triethoxy-silane. We studied the process related to PSi films (PSiFs), which were characterized by X-ray photoelectron spectroscopy (XPS), time of flight secondary ion mass spectroscopy (ToF-SIMS) and field emission scanning electron microscopy (FESEM) before and after a plasma patterning process. The analyses confirmed the surface oxidation and the anchorage of the organosilane backbone. We further highlighted the surface analytical potential of 13C, 19F and 29Si solid-state NMR (SS-NMR) as compared to Fourier transformed infrared spectroscopy (FTIR) in the characterization of functionalized PSi particles (PSiPs). The reduced invasiveness of the organosilanization regarding the PSiPs morphology was confirmed using transmission electron microscopy (TEM) and FESEM. Relevantly, the results obtained on PSiPs complemented those obtained on PSiFs. SS-NMR suggests a number of siloxane bonds between the organosilane and the PSiPs, which does not reach levels of maximum heterogeneous condensation, while ToF-SIMS suggested a certain degree of organosilane polymerization. Additionally, differences among the carbons in the organic (non-hydrolyzable) functionalizing groups are identified, especially in the case of the perfluorodecyl group. The spectroscopic characterization was used to propose a mechanism for the visible light activation of the organosilane assembly, which is based on the initial photoactivated oxidation of the PSi matrix.

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“…The reference spectrum of oxidized porous silicon support shows two bands corresponding to the characteristic asymmetric stretching mode of Si-O at 1,050 to 1,100 cm -1 and the Si-OH bond at 825 cm -1 [32]. The spectra of immobilized support show a sharp band of silanol at about 3,730 cm -1 and a band at 3,350 cm -1 correspond to the asymmetric stretching modes of -NH 2 groups.…”

Section: Resultsmentioning

confidence: 99%

Immobilization of peroxidase enzyme onto the porous silicon structure for enhancing its activity and stability

et al. 2014

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In this work, a commercial peroxidase was immobilized onto porous silicon (PS) support functionalized with 3-aminopropyldiethoxysilane (APDES) and the performance of the obtained catalytic microreactor was studied. The immobilization steps were monitored and the activity of the immobilized enzyme in the PS pores was spectrophotometrically determined. The enzyme immobilization in porous silicon has demonstrated its potential as highly efficient enzymatic reactor. The effect of a polar organic solvent (acetonitrile) and the temperature (up to 50°C) on the activity and stability of the biocatalytic microreactor were studied. After 2-h incubation in organic solvent, the microreactor retained 80% of its initial activity in contrast to the system with free soluble peroxidase that lost 95% of its activity in the same period of time. Peroxidase immobilized into the spaces of the porous silicon support would be perspective for applications in treatments for environmental security such as removal of leached dye in textile industry or in treatment of different industrial effluents. The system can be also applied in the field of biomedicine.

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Fabrication and characterization of a chemically oxidized-nanostructured porous silicon based biosensor implementing orienting protein A

Cited by 18 publications

References 23 publications

Gold nanoparticle triggered dual optoplasmonic-impedimetric sensing of prostate-specific antigen on interdigitated porous silicon platforms

Gold nanoparticle triggered dual optoplasmonic-impedimetric sensing of prostate-specific antigen on interdigitated porous silicon platforms

Visible Light Assisted Organosilane Assembly on Mesoporous Silicon Films and Particles

Immobilization of peroxidase enzyme onto the porous silicon structure for enhancing its activity and stability

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