Fabrication and characterization of a novel biporous spherical adsorbent for protein chromatography

Abstract

SummaryA novel rigid spherical biporous poly(glyciclyl methacrylate-triallyl isocyanurate-clivinyJbenzene) resin (denoted Resin B) has been fabricated by radical suspension copolymerization, with superfine granules of calcium carbonate, and toluene and n-heptane, as porogenic agents, as the basis of a novel porogenic mode, cooperation of solid granules and solvents. The pore structure, static adsorption behavior, and chromatographic properties of Resin B were characterized and compared with those of Resin A (with only solvents as porogens). The results indicated that the biporous resin is a promising chromatographic medium for highspeed protein separation with good mechanical performance and high dynamic adsorption capacity ancl column efficiency at high flow rates.

A novel matrix derivatized from hydrophilic gigaporous polystyrene-based microspheres for high-speed immobilized-metal affinity chromatography

“…The low back pressure of columns is an evidence for the presence of flow-through pores, which reduce the flow resistance. The same phenomenon has been reported with other macroporous beads [21,22]. In previous study [14], we have confirmed that mobile phase in the column flowed not only through the spaces between matrix but also through the gigapores of particles, i.e.…”

“…HETP was nearly independent of flow velocity in a wide range of flow rate. This is opposite to conventional porous medium columns, the HETPs of which strongly depend on flow velocity [21,22]. We speculate that the presence of flow-through pores in APS-Ni particles may induce intraparticle convective mass transfer for BSA and significantly reduce the stagnant mobile phase mass transfer of particles by reducing the diffusive distance [8].…”

Protein adsorption in two-dimensional electrochromatography packed with superporous and microporous cellulose beads

“…The q m of the DEAE-SC adsorbent was smaller than that of the DEAE-MC, indicating that the introduction of wide pores in the DEAE-SC resulted in the loss of specific surface area. That phenomenon was coincident with the typical features of the superporous matrix prepared by other methods [14,15]. As described by Liu et al, the adsorption capacity in protein electrochromatography was not influenced by the eEF.…”

Chromatographic Characterization and Process Performance of Column-Packed Anion Exchange Fibrous Adsorbents for High Throughput and High Capacity Bioseparations

“…For the fibrous column, when the flow velocity was higher than 75 cm/h, the plate height only increased slightly and nearly reached a plateau with a flow velocity up to 600 cm/h, i.e., the plate height is nearly independent of flow velocity for a wide range of flow rates. This behavior contrasts conventional porous mediums, like Q Sepharose FF columns, for which the plate height is strongly dependent on flow velocity [58,59]. The resultant theoretical plate height (H) (0.5-0.8 mm) and the measured peak asymmetric (As) values (≤1.6) of the fibrous column demonstrated good packing efficiency with good reproducibility and were comparable to bead packed columns.…”