Purpose:
To study the influence of silver nanoparticles (AgNP) on tissue reaction when incorporated into a polymeric matrix of polyacrylic acid-based (Carbopol
®
) gel as a proposal for a new low-cost type of biomaterial that is simple to manufacture for use as an antimicrobial and antioxidative dressing.
Methods:
In-vivo
tests of implantation in the subcutaneous tissue of the back of rats were performed using polyethylene tubes in three situations: empty, only the gel, and gel incorporated with AgNP. Then, the tissue reaction was studied by counting inflammatory cells. Additionally,
in-vitro
tests of the antioxidative and antimicrobial activity of AgNP were performed. The radical 2,2 diphenyl-1 picrylhydrazyl (DPPH) was used to test the antioxidative activity of AgNP using electron spin resonance. The antimicrobial activity of AgNP was determined by minimum inhibitory concentration against the microorganisms:
Staphylococcus aureus
,
Pseudomonas aeruginosa
, and
Escherichia coli
.
Results:
The results indicated that AgNP presents antioxidative activity and was able to inhibit the growth of the microorganisms tested. The addition of AgNP in Carbopol
®
did not alter the tissue inflammatory response (p
>
0.05, Kruskal-Wallis’s test).
Conclusions:
The new biomaterial is promising for future use as a dressing for its beneficial properties for regenerative processes.