Fabrication and characterization of chitosan/OGP coated porous poly(ε-caprolactone) scaffold for bone tissue engineering

Abstract

As one of the stimulators on bone formation, osteogenic growth peptide (OGP) improves both proliferation and differentiation of the bone cells in vitro and in vivo. The aim of this work was the preparation of three dimensional porous poly(ε-caprolactone) (PCL) scaffold with high porosity, well interpore connectivity, and then its surface was modified by using chitosan (CS)/OGP coating for application in bone regeneration. In present study, the properties of porous PCL and CS/OGP coated PCL scaffold, including the microstructure, water absorption, porosity, hydrophilicity, mechanical properties, and biocompatibility in vitro were investigated. Results showed that the PCL and CS/OGP-PCL scaffold with an interconnected network structure have a porosity of more than 91.5, 80.8%, respectively. The CS/OGP-PCL scaffold exhibited better hydrophilicity and mechanical properties than that of uncoated PCL scaffold. Moreover, the results of cell culture test showed that CS/OGP coating could stimulate the proliferation and growth of osteoblast cells on CS/OGP-PCL scaffold. These finding suggested that the surface modification could be a effective method on enhancing cell adhesion to synthetic polymer-based scaffolds in tissue engineering application and the developed porous CS/OGP-PCL scaffold should be considered as alternative biomaterials for bone regeneration.

3D Bone Biomimetic Scaffolds for Basic and Translational Studies with Mesenchymal Stem Cells

“…Therefore, the aim scientists have been pursuing, which has been driving a huge development of materials science area, was to achieve autologous growth or tissue regeneration by means of a replacement material closely resembling the natural one, alone or in combination with MSCs [ 15 ]. Namely, they sought to identify optimal materials displaying biocompatibility (i.e., incorporation into host tissue without activation of an adverse immune response), biodegradability (i.e., limited persistence in the host), osteoconductivity, and osteoinductivity (i.e., recruitment of osteoprogenitor cells and differentiation induction and support), in order to reproduce bone extracellular environment and, therein, physiological cell behavior [ 8 , 16 , 17 ].…”

Bioactive Sr(II)/Chitosan/Poly(ε-caprolactone) Scaffolds for Craniofacial Tissue Regeneration. In Vitro and In Vivo Behavior

“…Actually, some of them have recently developed. They can consist of blends [96,97], bilayer systems [98] and functionalized coatings [99]. Other systems have been prepared following nano [100], micro and macro approaches [101].…”

Biocompatible Synthetic Polymers for Tissue Engineering Purposes

“…As described in Section , surface properties are crucial since they affect the interactions of the implant with proteins and cells, and intermediate hydrophilicities are generally preferred to achieve such interactions . Some easy and efficient methods to improve the biocompatibility and tailor the hydrolytic degradation rates of PCL, PLA, and PLGA, are to increase their hydrophilicity by coating or blending with natural polymers, such as decellularized ECM, − collagen, − gelatin, ,,, elastin, , other proteins, ,, and polysaccharides. ,− Grafting and coating with hydrophilic synthetic polymers, such as poly­(glycerol sebacate) (PGS), , polyacrylamide, poly­(vinyl alcohol) (PVA), , poly­(ethylene oxide), polydopamine, polyurethane (PU) have also been performed, as well as plasma treatment. ,, Copolymerization and blending also help modulate the mechanical properties of these polymers. ,− Overall, PCL and PLA have been used in the TE of a wide variety of tissues, including cornea, skin, cartilage, and bone . The commercial availability and processability of such thermoplastic polyesters, combined with their established applications in products that have received FDA approval, are major factors that will, in our opinion, keep these polymers in the spotlight of the optimization of TE scaffolds.…”