Fabrication and characterization of gelatin‐based biocompatible porous composite scaffold for bone tissue engineering

Abstract

In this study, composite scaffolds were prepared with polyethylene oxide (PEO)-linked gelatin and tricalcium phosphate (TCP). Chitosan, a positively charged polysaccharide, was introduced into the scaffolds to improve the properties of the artificial bone matrix. The chemical and thermal properties of composite scaffolds were investigated by Fourier transform infrared spectroscopy, thermogravimetric analyzer, differential thermal analyzer. In vitro cytotoxicity of the composite scaffold was also evaluated and the sample showed no cytotoxic effect. The morphology was studied by SEM and light microscopy. It was observed that the prepared scaffold had an open interconnected porous structure with pore size of 230-354 μm, which is suitable for osteoblast cell proliferation. The mechanical properties were assessed and it was found that the composite had compressive modulus of 1200 MPa with a strength of 5.2 MPa and bending modulus of 250 MPa having strength of 12.3 MPa. The porosity and apparent density were calculated and it was found that the incorporation of TCP can reduce the porosity and water absorption. It was revealed from the study that the composite had a 3D porous microstructure and TCP particles were dispersed evenly among the crosslinked gelatin/chitosan scaffold.

Human periosteum cell osteogenic differentiation enhanced by ionic silicon release from porous amorphous silica fibrous scaffolds

“…Five samples per each time point were prepared in cylinder shape of approximately 6 mm in diameter and 12 mm in height in accordance with ASTM F 451–95 [26], all the calculations were done assuming that the section area of specimens remain unchanged during the test. Each Specimen was immersed into 1mL of α-MEM before the test.…”

Production and characterization of chitosan/gelatin/β-TCP scaffolds for improved bone tissue regeneration

“…However, when compared to the CH scaffolds, a statistically significant decrease in porosity was observed after the addition of β-TCP to the scaffold composition. Previous studies have shown that the porosity decreases with the increasing of ceramic particle content, since an increase in the density of the scaffold is verified [66]. In addition, the pore size of the scaffolds was further characterized by SEM analysis.…”

Food Functionality of Popular and Commonly Consumed Indigenous Vegetables and Fruits from Bangladesh

“…This functionality helps to determine anti tumor and chemotherapeutic agents [14]. In the study, it was found that, Pomelo has a very strong cytotoxic property with 100% mortality rate.…”

“…Vitamin C, Protein and Iron Content In vitro Cytotoxicity Test was performed using Brine Shrimp Lethality Bioassay method as described previously [14]. Previous method for Raw and fresh samples was also used to determine Vitamin C by HPLC [15].…”