Fabrication and characterization of polydopamine-mediated zein-based nanoparticle for delivery of bioactive molecules

Zhang, Zhiheng; Jiang, Han; Chen, Guo; Miao, Wenbo; Lin, Qianzhu; Sang, Shangyuan; McClements, David Julian; Jiao, Aiquan; Jin, Zhengyu; Wang, Jinpeng; Qiu, Chao

doi:10.1016/j.foodchem.2024.139477

Cited by 6 publications

(1 citation statement)

References 33 publications

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“…While PDA is typically chemically synthesized, which is not regarded as a green and sustainable process, − our synthetic protein-based biopolymers are biosynthesized by genetically engineered microorganisms in a sustainable and environmentally friendly manner. Both PDA and Cp19k-based biopolymers possess admirable adhesive and biocompatible properties. ,,− However, the functionality of PDA-based composite materials often relies on the combination of other components, such as polysaccharides and metals. − Furthermore, the lack of temperature-sensitive properties limits the applications of these PDA-based biomaterials. In contrast, our synthetic biopolymer exhibits a favorable temperature response, which holds the potential for use in smart, environment-responsive biomedical materials.…”

Section: Results and Discussionmentioning

confidence: 99%

Genetically Programmed Temperature-Responsive Barnacle-Derived Protein with an Enhanced Adhesion Ability

Shi,

Xu,

Zhang

et al. 2024

ACS Appl. Bio Mater.

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There is an emerging strong demand for smart environmentally responsive protein-based biomaterials with improved adhesion properties, especially underwater adhesion for potential environmental and medical applications. Based on the fusion of elastin-like polypeptides (ELPs), SpyCatcher and SpyTag modules, biosynthetic barnacle-derived protein was genetically engineered and self-assembled with an enhanced adhesion ability and temperature response. The water resistance ability of the synthetic protein biopolymer with a network structure increased to 98.8 from 58.5% of the original Cp19k, and the nonaqueous adhesion strength enhanced to 1.26 from 0.68 MPa of Cp19k. The biopolymer showed an improved adhesion ability toward hydrophilic and hydrophobic surfaces as well as diatomite powders. The combination of functional module ELPs and SpyTag/SpyCatcher could endow the biosynthetic protein with temperature response, an insoluble form above 42 °C and a soluble form at 4 °C. The combinational advantages including temperature response and adhesion performance make the self-assembled protein an excellent candidate in surgical adhesion, underwater repair, and surface modification of various coatings. Distinct from the traditional approach of utilizing solely ELPs, the integration of short ELPs with Spy partners exhibited a synergistic enhancement in the temperature response. The synergistic effects of two functional modules provide a technical method and insight for designing smart self-assembled protein-based biopolymers.

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