Fabrication and characterization of TGF-β1-loaded electrospun poly (lactic-co-glycolic acid) core-sheath sutures

Gu, Ziqi; Yin, Haiyue; Wang, Juan; Ma, Linlin; Morsi, Yos S.; Mo, Xiumei

doi:10.1016/j.colsurfb.2017.10.066

Cited by 33 publications

(16 citation statements)

References 40 publications

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“…The difficulty in vascular replacement can be attributed to multiple factors, including the limited availability of venous and arterial grafts and the lack of appropriate mechanical properties, chemical stimuli, and cell recruitment in synthetic vascular grafts. Most efforts to improve the strength and remodeling of synthetic vascular scaffolds have been directed toward fabricating the ideal biocompatible and biodegradable materials and providing appropriate biochemical environment and cell sources to enhance cellular organization [16][17][18]. Our in vitro studies using collagen gel showed that NCSC differentiation was regulated by substrate stiffness, and presented that matrix stiffness alone can promote a particular lineage over another, that is, SMCs on stiff substrates, and neural cells on soft substrates, which was consistent with in vivo differentiation of NCSCs [9].…”

Section: Discussionmentioning

confidence: 99%

Differentiation of Neural Crest Stem Cells in Response to Matrix Stiffness and TGF-β1 in Vascular Regeneration

et al. 2020

Stem Cells and Development

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The neural crest stem cells derived from human induced pluripotent stem cells (iPSC-NCSCs) are a valuable autologous cell source for tissue engineering and regenerative medicine. In this study, we investigated how iPSC-NCSCs could be regulated to regenerate arteries by microenvironmental factors, including the physical factor of matrix stiffness, and the chemical factor of transforming growth factor beta-1 (TGF-b1). We found that, compared to soft substrate, stiff substrate drove iPSC-NCSCs differentiation into smooth muscle cells, which was further enhanced by TGF-b1. To investigate the regulatory role of TGF-b1 in vivo, we fabricated vascular grafts composed of electrospun nanofibrous scaffolds, collagen gel, iPSC-NCSCs, and TGF-b1, and implanted them into athymic rats. The results showed that TGF-b1 significantly promoted extracellular matrix synthesis and increased mechanical strength of vascular grafts. This study presents a proof of concept that iPSC-NCSCs can be used as a promising autologous cell source for vascular regeneration when combined with physical and chemical engineering.

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Section: Discussionmentioning

confidence: 99%

Differentiation of Neural Crest Stem Cells in Response to Matrix Stiffness and TGF-β1 in Vascular Regeneration

et al. 2020

Stem Cells and Development

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“…To solve this problem, drugs and growth factors have been incorporated in core-sheath fibrous yarns to enhance mechanical strength and prevent microbial inhibition. Gu et al 8 concluded that summarized TGF-b1 loaded PLGA based micro-nano fiber core-sheath fibers imparted superior tensile strength and elongation without any fluctuation before breakage, compared to single polymer fibrous yarns. In addition, an in vitro release of TGF-b1 from PLGA core-sheath yarns in phosphate buffer saline (PBS) at 37 C, calculated based on the calibration curve of TGF-b1, revealed a burst release within 24 h followed by gradual elution of the growth factor.…”

Section: Suturesmentioning

confidence: 99%

“…There are compelling pieces of evidence that core-sheath fibers and core-sheath fiber-based architectures (e.g., scaffolds) made significant breakthrough and progress over the years in the field of biomedical and tissue engineering. [3][4][5][6][7][8][9] They served as a precious gemstone (Kohinoor diamond) in regenerative medicine with the goal of repairing and reconstructing diseased and defective tissues. Primarily, the form and nature of culture scaffolds are important in controlling cell behavior and constructing tissues from individual cells.…”

Section: Introductionmentioning

confidence: 99%

Current methodologies and approaches for the formation of core–sheath polymer fibers for biomedical applications

Muthusubramanian

Edirisinghe

Homer‐Vanniasinkam

et al. 2020

Applied Physics Reviews

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The application of polymer fibers has rocketed to unimaginable heights in recent years and occupies every corner of our day-to-day life, from knitted protective textile clothes to buzzing smartphone electronics. Polymer fibers could be obtained from natural and synthetic polymers at a length scale from the nanometer to micrometer range. These fibers could be formed into different configurations such as single, core-sheath, hollow, blended, or composite according to human needs. Of these several conformations of fibers, core-sheath polymer fibers are an interesting class of materials, which shows superior physical, chemical, and biological properties. In core-sheath fiber structures, one of the components called a core is fully surrounded by the second component known as a sheath. In this format, different polymers can be applied as a sheath over a solid core of another polymer, thus resulting in a variety of modified properties while maintaining the major fiber property. After a brief introduction to core-sheath fibers, this review paper focuses on the development of the electrospinning process to manufacture core-sheath fibers followed by illustrating the current methodology and approaches to form them on a larger scale, suitable for industrial manufacturing and exploitation. Finally, the paper reviews the applications of the core-sheath fibers, in particular, recent studies of core-sheath polymer fibers in tissue engineering (nerve, vascular grafts, cardiomyocytes, bone, tendons, sutures, and wound healing), growth factors and other bioactive component release, and drug delivery. Therefore, core-sheath structures are a revolutionary development in the field of science and technology, becoming a backbone to many emerging technologies and novel opportunities.

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“…21 Recently, many applications have been reported for the core-sheath yarns. According to the previous research studies, some applications include functional sutures, 22,23 wearable piezoelectric nanogenerators, 24 heat storage systems, 25 energy harvesting devices, 26 supercapacitors, 6,27 and tissue engineering scaffolds. 9 Zhao et al investigated the application of the core-sheath yarns in the woven filters.…”

Section: Introductionmentioning

confidence: 99%

Investigating the wicking behavior of micro/nanofibrous core‐sheath PET–PAN yarn modified by dimethyl 5‐sodium sulfoisophthalate

Abbaszadeh

Ravandi

Valipouri

et al. 2019

J of Applied Polymer Sci

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The present research aims at imparting an improved wicking ability to the recycled multifilament yarns by wrapping composite nanofibers to attain an efficient material for filtration purposes. Therefore, polyacrylonitrile nanofibers containing dimethyl 5-sodium sulfoisophthalate nanoparticles were wrapped around the recycled poly(ethylene terephthalate) yarn by means of a novel electrospinning technique. Several tests were performed to investigate the parameters affecting wicking rise and moisture regain of the samples. Taguchi method was used in two separate designs (with or without nanoparticles). Some factors such as polymer solution concentration, mass ratio of nanoparticles, take-up rate, and number of filaments were considered as the variable factors while yarn wicking rate and moisture regain were the response factors. It was found that the hydrophilic nature of nanoparticles together with the ultrafine structure of nanofibers provide yarns with enhanced wicking properties. Although solution concentration is the predominant factor in wicking rate of the yarns containing nanoparticles, the most effective factor in wicking rise and moisture regain for other cases is the number of filaments. The mechanism of nanoparticle effect on fluidic jet during electrospinning process is explained by theory of nanofluids stability which has never been validated experimentally in previous research studies.

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Fabrication and characterization of TGF-β1-loaded electrospun poly (lactic-co-glycolic acid) core-sheath sutures

Cited by 33 publications

References 40 publications

Differentiation of Neural Crest Stem Cells in Response to Matrix Stiffness and TGF-β1 in Vascular Regeneration

Differentiation of Neural Crest Stem Cells in Response to Matrix Stiffness and TGF-β1 in Vascular Regeneration

Current methodologies and approaches for the formation of core–sheath polymer fibers for biomedical applications

Investigating the wicking behavior of micro/nanofibrous core‐sheath PET–PAN yarn modified by dimethyl 5‐sodium sulfoisophthalate

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