Fabrication and development of a microfluidic paper-based immunosorbent assay platform (μPISA) for colorimetric detection of hepatitis C

Ozefe, Fatih; Yıldız, Ahu Arslan

doi:10.1039/d2an01761j

Cited by 4 publications

(2 citation statements)

References 59 publications

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“…With the use of a paper-based microdevice, the sample volume needed for analysis was only 5 μL, and the results were validated by naked-eye readout within 30 min of analysis. Ozefe et al also developed a micro-paper ELISA (µPISA) for rapid detection of the hepatitis C virus (HCV), and the LOD was approximately 10 ng/mL for HCV when a human blood plasma sample was used [ 54 ].…”

Section: Virus Detection On Microfluidic Platformmentioning

confidence: 99%

Recent Advances in Molecular and Immunological Diagnostic Platform for Virus Detection: A Review

2023

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Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has caused an ongoing coronavirus disease (COVID-19) outbreak and a rising demand for the development of accurate, timely, and cost-effective diagnostic tests for SARS-CoV-2 as well as other viral infections in general. Currently, traditional virus screening methods such as plate culturing and real-time PCR are considered the gold standard with accurate and sensitive results. However, these methods still require sophisticated equipment, trained personnel, and a long analysis time. Alternatively, with the integration of microfluidic and biosensor technologies, microfluidic-based biosensors offer the ability to perform sample preparation and simultaneous detection of many analyses in one platform. High sensitivity, accuracy, portability, low cost, high throughput, and real-time detection can be achieved using a single platform. This review presents recent advances in microfluidic-based biosensors from many works to demonstrate the advantages of merging the two technologies for sensing viruses. Different platforms for virus detection are classified into two main sections: immunoassays and molecular assays. Moreover, available commercial sensing tests are analyzed.

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Section: Virus Detection On Microfluidic Platformmentioning

confidence: 99%

Recent Advances in Molecular and Immunological Diagnostic Platform for Virus Detection: A Review

2023

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“…24,25 Numerous studies have exploited PADs to develop point-of-care and POU testing platforms. 26 Such testing systems have been performed using immunosorbents, 27 molecularly imprinted sorbents, 28 or hydrophobic paper 29 coupled with colorimetric or spectrouorimetric detection. Nevertheless, we performed a literature survey that revealed that no PAD is available for the POU determination of the water content in ethanol.…”

Section: Introductionmentioning

confidence: 99%

Simple colorimetric paper-based test strip for point-of-use quality testing of ethanol-based hand sanitizers

El-Hassanein,

Mansour,

Hammad

et al. 2024

RSC Adv.

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Origami-inspired microfluidic paper-based analytical device (μPAD) for microorganism detection

Sözmen,

Bayraktar,

Arslan-Yildiz

2024

emergent mater.

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Pathogenic microorganisms impose great risk especially in resource-limited settings due to inaccessibility of diagnostic tools and monitoring devices. This is mainly caused by current methods often being economically demanding and complex in practice; while these methods are sensitive and accurate, they rarely follow Point-of-care (POC) approaches, which is essential for rapid detection and intervention. Incorporating origami into paper-based analytical devices (μPAD) presents an innovative alternative, offering affordability, portability, and ease of disposal. Herein, a colorimetric origami μPAD that is suitable for use in POC applications was developed. The μPAD was fabricated via laser ablation utilizing PVDF and cellulose membranes. In order to develop the biosensor platform, fabrication parameters were optimized and hydrophilicity of PVDF membranes was improved using various solvents. The PVDF membranes were characterized through light microscopy imaging, protein adsorption assay and contact angle measurements. Then, optimization of the assay parameters was carried out in order to improve sensitivity and resolution of the μPAD, utilizing Box-Behnken experimental design. The responses generated by the origami μPAD in form of visible color development were then analyzed using image processing. After optimization is concluded, E. coli detection was carried out as a model system. Resulting calculations showed a limit of detection (LoD) of 2 CFU/mL and a dynamic working range up to 106 CFU/mL for E. coli. Overall, developed origami μPAD promises an economic advantage compared to conventional methods, and provides rapid and sensitive results without the requirement of expertise or complex equipment.

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Fabrication and development of a microfluidic paper-based immunosorbent assay platform (μPISA) for colorimetric detection of hepatitis C

Abstract: Paper-based microfluidics is an emerging analysis tool used in various applications, especially in point-of-care (PoC) diagnostic applications, due to its advantages over other types of microfluidic devices in terms of...

Cited by 4 publications

References 59 publications

Recent Advances in Molecular and Immunological Diagnostic Platform for Virus Detection: A Review

Recent Advances in Molecular and Immunological Diagnostic Platform for Virus Detection: A Review

Simple colorimetric paper-based test strip for point-of-use quality testing of ethanol-based hand sanitizers

Origami-inspired microfluidic paper-based analytical device (μPAD) for microorganism detection

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