Fabrication and evaluation of an optimized acellular nerve allograft with multiple axial channels

Yu, Tianhao; Wen, Lei; He, Jing; Xu, Yingxi; Li, Ting; Wang, Weizuo; Ma, Yizhan; Ahmad, Mukhtar; Tian, Xiaohong; Fan, Jun; Wang, Xiaohong; Hagiwara, Haruo; Ao, Qiang

doi:10.1016/j.actbio.2020.07.059

Cited by 27 publications

(21 citation statements)

References 68 publications

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“…The main reason for undertaking such types of protocol is to protect biodiversity from the impacts of global warming [ 8 – 10 ]. Impacts of climate change has been reported for humans as well as biodiversity, including disease [ 11 , 12 ] and pest outbreaks [ 13 ], shift in species distribution, occurrence and Phenology [ 14 ]. The condition is predicted to become spectacular as the magnitude of environmental change accelerates [ 15 ].…”

Section: Introductionmentioning

confidence: 99%

Modeling potential distribution of newly recorded ant, Brachyponera nigrita using Maxent under climate change in Pothwar region, Pakistan

et al. 2022

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Climate change has been discussed as to exert shifts in geographical range of plants, animals or insect species by increasing, reducing or shifting its appropriate climatic habitat. Globally, Pakistan has been ranked at 5th position on the list of countries most vulnerable to climate change in 2020. Climate change has resulted in the losses of biodiversity and alteration in ecosystem as a result of depletion of natural habitats of species in Pakistan as well as in the world. Ants have been regarded as indicators of environmental change and ecosystem processes. Brachyponera nigrita (Emery, 1895) was reported for the first time from Pakistan (Pothwar region). Objective of our studies was to model geographic distribution of newly recorded ant species, B. nigrita based on two representative concentration pathways (RCP) (RCP4.5 and RCP8.5) for 2050s using maximum entropy model (Maxent) in Pakistan. In modeling procedure, 21occurrence records and 8 variables namely Bio4 (Temperature seasonality), Bio8 (Mean temperature of wettest quarter), Bio10 (Mean temperature of warmest quarter), Bio12 (Annual precipitation), Bio13 (Precipitation of wettest month), Bio15 (Precipitation seasonality), Bio17 (Precipitation of driest quarter) and Bio18 (Precipitation of warmest quarter) were used to determine the current and future distributions. Performance of the model was evaluated using AUC (area under curves) values, partial ROC, omission rates (E = 5%) and AICc (Model complexity).The results showed the average AUC value of the model was 0.930, which indicated that the accuracy of the model was excellent. The jackknife test also showed that Bio4, Bio18, Bio17 and Bio15 contributed 98% for the prediction of potential distribution of the species as compared to all other variables. Maxent results indicated that distribution area of B. nigrita under future predicted bioclimatics 2050 (RCP 4.5 and RCP8.5) would be increased in various localities of Pakistan as compared to its current distribution. In Pothwar region, moderately suitable and highly suitable areas of this species would increase by 505.932321km2and 572.118421km2as compared to current distribution under 2050 (RCP 4.5), while under 2050 (RCP 8.5), there would be an increase of 6427.2576km2and 3765.140493km2 respectively in moderately suitable and highly suitable areas of B. nigrita. This species was associated with termites, collembolans and larval stages of different insects. White eggs, creamy white pupae and many workers of this species were observed in a variety of habitats. Unknown nesting ecology, species identification characters supported with micrographs has been given which will help researchers for further ecological studies.

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Section: Introductionmentioning

confidence: 99%

Modeling potential distribution of newly recorded ant, Brachyponera nigrita using Maxent under climate change in Pothwar region, Pakistan

et al. 2022

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“…Previous studies investigating nerve decellularization mainly used 3% Triton™ X-100 and 4% SDC for up to 72 h [ 42 , 43 , 44 , 45 , 46 , 47 , 48 , 49 ]. By following our hypothesis of combining detergents, we could reduce these concentrations to 1% for Triton™ X-100 and 2% for SDC.…”

Section: Discussionmentioning

confidence: 99%

Efficacy of Nerve-Derived Hydrogels to Promote Axon Regeneration Is Influenced by the Method of Tissue Decellularization

Kuna

Lundgren

Anerillas

et al. 2022

IJMS

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Injuries to large peripheral nerves are often associated with tissue defects and require reconstruction using autologous nerve grafts, which have limited availability and result in donor site morbidity. Peripheral nerve-derived hydrogels could potentially supplement or even replace these grafts. In this study, three decellularization protocols based on the ionic detergents sodium dodecyl sulfate (P1) and sodium deoxycholate (P2), or the organic solvent tri-n-butyl phosphate (P3), were used to prepare hydrogels. All protocols resulted in significantly decreased amounts of genomic DNA, but the P2 hydrogel showed the best preservation of extracellular matrix proteins, cytokines, and chemokines, and reduced levels of sulfated glycosaminoglycans. In vitro P1 and P2 hydrogels supported Schwann cell viability, secretion of VEGF, and neurite outgrowth. Surgical repair of a 10 mm-long rat sciatic nerve gap was performed by implantation of tubular polycaprolactone conduits filled with hydrogels followed by analyses using diffusion tensor imaging and immunostaining for neuronal and glial markers. The results demonstrated that the P2 hydrogel considerably increased the number of axons and the distance of regeneration into the distal nerve stump. In summary, the method used to decellularize nerve tissue affects the efficacy of the resulting hydrogels to support regeneration after nerve injury.

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“…Anisotropic hydrogel scaffolds with oriented pores or channels provide guidance cues for cells, affecting their growth direction and rate. Studies have shown that anisotropic hydrogels were advantageous for PNI and SCI treatment because they promote glial cell and axonal alignment, outgrowth, and myelination [ [182] , [183] , [184] , [185] , [186] , [187] ]. De Laporte and co-workers defined the term Anisogel, where a soft hydrogel surrounded magneto-responsive short fibers that were directionally oriented [ 157 ].…”

Section: Anisotropic 3d Hydrogel Scaffoldsmentioning

confidence: 99%

Anisotropic scaffolds for peripheral nerve and spinal cord regeneration

Xue

Shi

Kong

et al. 2021

Bioactive Materials

112

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The treatment of long-gap (>10 mm) peripheral nerve injury (PNI) and spinal cord injury (SCI) remains a continuous challenge due to limited native tissue regeneration capabilities. The current clinical strategy of using autografts for PNI suffers from a source shortage, while the pharmacological treatment for SCI presents dissatisfactory results. Tissue engineering, as an alternative, is a promising approach for regenerating peripheral nerves and spinal cords. Through providing a beneficial environment, a scaffold is the primary element in tissue engineering. In particular, scaffolds with anisotropic structures resembling the native extracellular matrix (ECM) can effectively guide neural outgrowth and reconnection. In this review, the anatomy of peripheral nerves and spinal cords, as well as current clinical treatments for PNI and SCI, is first summarized. An overview of the critical components in peripheral nerve and spinal cord tissue engineering and the current status of regeneration approaches are also discussed. Recent advances in the fabrication of anisotropic surface patterns, aligned fibrous substrates, and 3D hydrogel scaffolds, as well as their in vitro and in vivo effects are highlighted. Finally, we summarize potential mechanisms underlying the anisotropic architectures in orienting axonal and glial cell growth, along with their challenges and prospects.

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Fabrication and evaluation of an optimized acellular nerve allograft with multiple axial channels

Cited by 27 publications

References 68 publications

Modeling potential distribution of newly recorded ant, Brachyponera nigrita using Maxent under climate change in Pothwar region, Pakistan

Modeling potential distribution of newly recorded ant, Brachyponera nigrita using Maxent under climate change in Pothwar region, Pakistan

Efficacy of Nerve-Derived Hydrogels to Promote Axon Regeneration Is Influenced by the Method of Tissue Decellularization

Anisotropic scaffolds for peripheral nerve and spinal cord regeneration

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