Fabrication and fluorescence imaging properties of indocyanine green-loaded poly(lactic-co-glycolic acid) nanoparticles

Tayama, Mayu; Inose, Tomoya; Yamauchi, Noriko; Nakashima, Kouichi; Tokunaga, Masayuki; Kato, C.; Gonda, Kohsuke; Kobayashi, Yoshio

doi:10.1007/s00396-021-04844-3

Cited by 3 publications

(3 citation statements)

References 33 publications

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“…When the tumor volume reached 100 mm 3 , Cy5-DMPM was injected into the tail vein of the mice and imaged using IVIS at different time points (0.5, 1, 2, 4, 6, 8, 12, and 24 h). 68 In addition, to examine the in vivo enrichment of DMPM in tumors, the nude mice were euthanized 24 h after the injection of Cy5-DMPM into the tail vein, and the major organs, such as heart, liver, spleen, lung, and kidney, and tumor tissues were imaged using IVIS in vitro. The fluorescence imaging data were analyzed using IVIS and the Image-J software.…”

Section: Methodsmentioning

confidence: 99%

“…To further confirm the tumor-targeting effects of MA-DMPM in vivo , the tumor model of NCI-H1299 cell was used and investigated by monitoring the fluorescence distribution of Cy5-labeled DMPM (Cy5-DMPM). When the tumor volume reached 100 mm 3 , Cy5-DMPM was injected into the tail vein of the mice and imaged using IVIS at different time points (0.5, 1, 2, 4, 6, 8, 12, and 24 h) . In addition, to examine the in vivo enrichment of DMPM in tumors, the nude mice were euthanized 24 h after the injection of Cy5-DMPM into the tail vein, and the major organs, such as heart, liver, spleen, lung, and kidney, and tumor tissues were imaged using IVIS in vitro .…”

Section: Methodsmentioning

confidence: 99%

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Pluronic-Based Nanoparticles for Delivery of Doxorubicin to the Tumor Microenvironment by Binding to Macrophages

Hu,

Wang,

Gao

et al. 2024

ACS Nano

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The active targeting drug delivery system based on special types of endogenous cells such as macrophages has emerged as a promising strategy for tumor therapy, owing to its tumor homing property and biocompatibility. In this work, the active tumor-targeting drug delivery system carrying doxorubicin-loaded nanoparticles (DOX@MPF127-MCP-1, DMPM) on macrophage (RAW264.7) surfaces via the mediation of interaction with the CCR2/MCP-1 axis was exploited. Initially, the amphiphilic block copolymer Pluronic F127 (PF127) was carboxylated to MPF127 at the hydroxyl terminus. Subsequently, MPF127 was modified with MCP-1 peptide to prepare MPF127-MCP-1 (MPM). The DOX was wrapped in MPM to form DMPM nanomicelles (approximately 100 nm) during the self-assembly process of MPM. The DMPM spontaneously bound to macrophages (RAW264.7), which resulted in the construction of an actively targeting delivery system (macrophage-DMPM, MA-DMPM) in vitro and in vivo. The DOX in MA-DMPM was released in the acidic tumor microenvironment (TME) in a pH-responsive manner to increase DOX accumulation and enhance the tumor treatment effect. The ratio of MA-DMPM homing reached 220% in vitro compared with the control group, indicating that the MA-DMPM was excellently capable of tumor-targeting delivery. In in vivo experiments, nonsmall cell lung cancer cell (NCI-H1299) tumor models were established. The results of the fluorescence imaging system (IVIS) showed that MA-DMPM demonstrated tremendous tumor-targeting ability in vivo. The antitumor effects of MA-DMPM in vivo indicated that the proportion of tumor cell apoptosis in the DMPM-treated group was 63.33%. The findings of the tumor-bearing mouse experiment proved that MA-DMPM significantly suppressed tumor cell growth, which confirmed its immense potential and promising applications in tumor therapy.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Pluronic-Based Nanoparticles for Delivery of Doxorubicin to the Tumor Microenvironment by Binding to Macrophages

Hu,

Wang,

Gao

et al. 2024

ACS Nano

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“…In 2021, Mayu Tayama et al wrapped indocyanine green (ICG) in PLGA and effectively prevented ICG from aggregation. 10 Thus, to improve the fluorescence imaging effect, nanoprobes are critical.…”

Section: Introductionmentioning

confidence: 99%

pH-Responsive nanoprobes for tumour fluorescence imaging based on spirolactam rhodamine

Sha

Wang

et al. 2022

Mater. Chem. Front.

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Synthesis of gold nanoparticle-immobilized biodegradable polymer nanoparticles and their degradation properties

Akino,

Takahashi,

Yamauchi

et al. 2024

Chem. Pap.

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Fabrication and fluorescence imaging properties of indocyanine green-loaded poly(lactic-co-glycolic acid) nanoparticles

Cited by 3 publications

References 33 publications

Pluronic-Based Nanoparticles for Delivery of Doxorubicin to the Tumor Microenvironment by Binding to Macrophages

Pluronic-Based Nanoparticles for Delivery of Doxorubicin to the Tumor Microenvironment by Binding to Macrophages

pH-Responsive nanoprobes for tumour fluorescence imaging based on spirolactam rhodamine

Synthesis of gold nanoparticle-immobilized biodegradable polymer nanoparticles and their degradation properties

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