Fabrication and hemocompatibility of cell outer membrane mimetic surfaces on chitosan by layer by layer assembly with polyanion bearing phosphorylcholine groups

Gong, Mali; Wang, Yanbing; Li, Ming; Hu, Bi−Huang; Gong, Yong‐Kuan

doi:10.1016/j.colsurfb.2010.10.049

Cited by 42 publications

(26 citation statements)

References 41 publications

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“…The platelet adhesion test was performed under static conditions according to a previously reported method . Whole blood (4 mL) was collected from a healthy donor in a polyethylene disposable syringe containing 444 μL of a 3.8 wt % aqueous sodium citrate solution.…”

Section: Methodsmentioning

confidence: 89%

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Surface reconstruction and hemocompatibility improvement of a phosphorylcholine end‐capped poly(butylene succinate) coating

Hao

Wang

Zhang

et al. 2013

J Biomedical Materials Res

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Control over cell-material surface interactions is the key to many new and improved biomedical devices. In this study, we present a simple yet effective surface modification method that allows for the surface reconstruction and formation of cell outer membrane mimetic structure on coatings that have significantly increased hemocompatibility. To achieve this, a phosphorylcholine end-capped poly(butylene succinate) (PBS-PC) was synthesized and dip-coated on coverslips. The surface structure of the amphiphilic PBS-PC film was reconstructed by heating in a vacuum oven to obtain the less hydrophilic surface and by immersing in hot water to obtain the more hydrophilic surface. Significant changes in the surface element concentration were observed by X-ray photoelectron spectroscopy analysis and changes in surface wettability were measured by sensitive dynamic contact angle technique. Scanning electron microscope images showed different morphologies of the reconstructed surfaces. Interestingly, the reconstruction between the less hydrophilic and more hydrophilic surfaces is reversible. More importantly, both the reconstructed surfaces are stable in room condition for more than 6 months, and both the surfaces show significant improvement in hemocompatibility as revealed by protein adsorption and platelet adhesion measurements. This reversible surface reconstruction strategy and the interesting results may be significant for fabricating stable and hemocompatible surfaces on differently shaped biomedical devices.

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Section: Methodsmentioning

confidence: 89%

“…In vitro single protein adsorption experiments were performed in phosphate‐buffered saline (pH 7.4) as described elsewhere . Briefly, the samples (18 × 18 mm) were firstly equilibrated with phosphate‐buffered saline for 2 h, and then immersed in a solution of BSA (4.5 mg/mL) or Fg (0.3 mg/mL) at 37°C.…”

Section: Methodsmentioning

confidence: 99%

Surface reconstruction and hemocompatibility improvement of a phosphorylcholine end‐capped poly(butylene succinate) coating

Hao

Wang

Zhang

et al. 2013

J Biomedical Materials Res

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“…When a surface‐based device is in contact with blood, plasma proteins adsorb in seconds to the surface, followed by platelet thrombus formation . Therefore, platelet adhesion on the substrate is another important tool for the evaluation of protein repellent ability.…”

Section: Resultsmentioning

confidence: 99%

A New Strategy to Prepare Poly(2‐methyl‐2‐oxazoline)‐Based Antifouling Coatings: Using UV‐Crosslinked Copolymer Anchors

Zhu

Chen

Zhang

et al. 2019

Macro Materials & Eng

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A series of the copolymer, poly(styrene-random-glycidyl methacrylate) (P(Str-GMA)), is synthesized by free radical polymerization, and characterized by 1 H NMR spectroscopy and gel permeation chromatography. The various substrates are then modified by P(St-r-GMA) under ultraviolet (UV) irradiation. Subsequently, the poly(2-methyl-2-oxazoline) (PMOXA) based coatings are prepared by anchoring amino-terminated PMOXA onto the P(St-r-GMA) modified surfaces through the reaction between the amino group of PMOXA and epoxy group of P(St-r-GMA). The results of ellipsometry, X-ray photoelectron spectroscopy, atomic force microscopy, and water contact angle reveal that PMOXA-based coatings can be prepared successfully on the substrates through UV-crosslinked P(St-r-GMA) as anchoring coatings. Besides, the PMOXA-based coatings display not only a superior antifouling property but long-term stability as well. Furthermore, the location of the coating formed on the substrate can be well controlled through selecting the site of UV irradiation, which can be utilized for the selectivity of protein adsorption (or resistance) on special devices.

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“…P(VDMA‐ co ‐MPC) copolymers are of interest due to the anti‐fouling, nonthrombogenic properties of MPC 32, 33. Despite initial preparative challenges, these copolymers proved to be very hydrophilic and good candidates for network formation.…”

Section: Resultsmentioning

confidence: 99%

Synthesis and properties of water‐soluble azlactone copolymers

Gardner

Brown

Stöver

2012

J. Polym. Sci. A Polym. Chem.

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Preparation and study of a series of copolymers incorporating 2-vinyl-4,4-dimethylazlactone (VDMA) is reported. The reactivity ratios for photo-initiated free radical copolymerization of VDMA with methacrylic acid (MAA), acrylic acid (AA), acrylamide (AAm), dimethylacrylamide (DMAA), hydroxyethyl methacrylate (HEMA), methoxy poly(ethylene glycol) methacrylate (MPEG 300 MA), and 2-methacryloyloxyethyl phosphorylcholine (MPC), were determined by fitting comonomer conversion data obtained by in situ 1 H NMR to a terminal copolymerization equation. Semi-batch photo-copolymerizations were then used to synthesize the corresponding VDMA copolymers with constant composition. Their solubility and dissolution behavior, as well as their hydrolysis half-lives under physiological conditions, were determined. P(VDMA-co-MAA) copolymers with 52 to 93 mol % VDMA showed decreasing initial solubility and increasing hydrolysis half-lives with increasing VDMA content. VDMA copolymers with nonionic monomers AAm and DMAA were water soluble only at VDMA contents of 41 and 22 mol % or less, respectively, and showed longer hydrolysis half-lives than comparable MAA copolymers. VDMA copolymers with HEMA and MPEG 300 MA were found to crosslink during storage, so their hydrolysis half-lives were not determined. VDMA copolymers with 18% zwitterionic MPC showed a much longer half-life and superior initial solubility compared to analogous p(VDMAco-MAA), identifying this copolymer as a promising candidate for macromolecular crosslinkers in, for example, aqueous layerby-layer co-depositions with polyamines. V C 2012 Wiley Periodicals, Inc. J Polym Sci Part A: Polym Chem 50: [4674][4675][4676][4677][4678][4679][4680][4681][4682][4683][4684][4685] 2012

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Fabrication and hemocompatibility of cell outer membrane mimetic surfaces on chitosan by layer by layer assembly with polyanion bearing phosphorylcholine groups

Cited by 42 publications

References 41 publications

Surface reconstruction and hemocompatibility improvement of a phosphorylcholine end‐capped poly(butylene succinate) coating

Surface reconstruction and hemocompatibility improvement of a phosphorylcholine end‐capped poly(butylene succinate) coating

A New Strategy to Prepare Poly(2‐methyl‐2‐oxazoline)‐Based Antifouling Coatings: Using UV‐Crosslinked Copolymer Anchors

Synthesis and properties of water‐soluble azlactone copolymers

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