Fabrication and Printing of Multi-material Hydrogels

Arumugasaamy, Navein; Baker, Hannah B.; Kaplan, David S.; Kim, Peter C.W.; Fisher, John P.

doi:10.1007/978-3-319-40498-1_13-1

Cited by 3 publications

(2 citation statements)

References 125 publications

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“…B. et al, 2016 ; Naghieh et al, 2018a ; Sarker et al, 2018 ; Sadeghianmaryan et al, 2020 ) or by thermal gelation ( e.g. , for collagen) ( Arumugasaamy et al, 2017 ). When bioinks are used, crosslinker concentration must be sufficient to print structures with high printability and cell viability ( Ashammakhi et al, 2019 ).…”

Section: Bone Structure and Defectsmentioning

confidence: 99%

3D Bioprinted Scaffolds for Bone Tissue Engineering: State-Of-The-Art and Emerging Technologies

Yazdanpanah

Johnston

Cooper

et al. 2022

Front. Bioeng. Biotechnol.

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Treating large bone defects, known as critical-sized defects (CSDs), is challenging because they are not spontaneously healed by the patient’s body. Due to the limitations associated with conventional bone grafts, bone tissue engineering (BTE), based on three-dimensional (3D) bioprinted scaffolds, has emerged as a promising approach for bone reconstitution and treatment. Bioprinting technology allows for incorporation of living cells and/or growth factors into scaffolds aiming to mimic the structure and properties of the native bone. To date, a wide range of biomaterials (either natural or synthetic polymers), as well as various cells and growth factors, have been explored for use in scaffold bioprinting. However, a key challenge that remains is the fabrication of scaffolds that meet structure, mechanical, and osteoconductive requirements of native bone and support vascularization. In this review, we briefly present the latest developments and discoveries of CSD treatment by means of bioprinted scaffolds, with a focus on the biomaterials, cells, and growth factors for formulating bioinks and their bioprinting techniques. Promising state-of-the-art pathways or strategies recently developed for bioprinting bone scaffolds are highlighted, including the incorporation of bioactive ceramics to create composite scaffolds, the use of advanced bioprinting technologies (e.g., core/shell bioprinting) to form hybrid scaffolds or systems, as well as the rigorous design of scaffolds by taking into account of the influence of such parameters as scaffold pore geometry and porosity. We also review in-vitro assays and in-vivo models to track bone regeneration, followed by a discussion of current limitations associated with 3D bioprinting technologies for BTE. We conclude this review with emerging approaches in this field, including the development of gradient scaffolds, four-dimensional (4D) printing technology via smart materials, organoids, and cell aggregates/spheroids along with future avenues for related BTE.

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Section: Bone Structure and Defectsmentioning

confidence: 99%

3D Bioprinted Scaffolds for Bone Tissue Engineering: State-Of-The-Art and Emerging Technologies

Yazdanpanah

Johnston

Cooper

et al. 2022

Front. Bioeng. Biotechnol.

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“…In particular, for bone regeneration applications, bioprinting entails the use of GelMA as a cell-laden bioink with a scaffolding structure as a mechanical backbone [ 32 , 107 , 108 , 109 ]. The encapsulation of the cells within the hydrogel matrix provides a transient native tissue mimicking microenvironment for the cells to interact with a mechanically compatible scaffold instead of relying on cell-scaffold interaction via cell adhesion [ 110 ]. The printed constructs are then implanted directly post-fabrication or in some cases are cultured in vitro to allow tissue maturation before implantation in vivo.…”

Section: Applications Of Gelma With a Focus On Musculoskeletal Regene...mentioning

confidence: 99%

Gelatin Methacryloyl Hydrogels for Musculoskeletal Tissue Regeneration

et al. 2022

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Musculoskeletal disorders are a significant burden on the global economy and public health. Hydrogels have significant potential for enhancing the repair of damaged and injured musculoskeletal tissues as cell or drug delivery systems. Hydrogels have unique physicochemical properties which make them promising platforms for controlling cell functions. Gelatin methacryloyl (GelMA) hydrogel in particular has been extensively investigated as a promising biomaterial due to its tuneable and beneficial properties and has been widely used in different biomedical applications. In this review, a detailed overview of GelMA synthesis, hydrogel design and applications in regenerative medicine is provided. After summarising recent progress in hydrogels more broadly, we highlight recent advances of GelMA hydrogels in the emerging fields of musculoskeletal drug delivery, involving therapeutic drugs (e.g., growth factors, antimicrobial molecules, immunomodulatory drugs and cells), delivery approaches (e.g., single-, dual-release system), and material design (e.g., addition of organic or inorganic materials, 3D printing). The review concludes with future perspectives and associated challenges for developing local drug delivery for musculoskeletal applications.

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In Vitro Models for Studying Transport Across Epithelial Tissue Barriers

et al. 2018

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Epithelial barriers are the body's natural defense system to regulating passage from one domain to another. In our efforts to understand what can and cannot cross these barriers, models have emerged as a reductionist approach to rigorously study and investigate this question. In particular, in vitro tissue models have become prominent as there is an increased exploration of understanding biological molecular transport. Herein, we introduce the pertinent physiology, then discuss recent studies and approaches for building models of five epithelial tissues: skin, the gastrointestinal tract, the lungs, the blood-brain barrier, and the placenta. In particular, we evaluated literature from the past 5 years utilizing a tissue model to evaluate molecular transport. We then compare physiology of these tissues and discuss similarities in approaches, across tissues, to validate these models. We conclude with a summary of the approaches of growing interest across multiple tissues and an outlook on future steps to improve these models.

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Fabrication and Printing of Multi-material Hydrogels

Cited by 3 publications

References 125 publications

3D Bioprinted Scaffolds for Bone Tissue Engineering: State-Of-The-Art and Emerging Technologies

3D Bioprinted Scaffolds for Bone Tissue Engineering: State-Of-The-Art and Emerging Technologies

Gelatin Methacryloyl Hydrogels for Musculoskeletal Tissue Regeneration

In Vitro Models for Studying Transport Across Epithelial Tissue Barriers

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