Fabrication, characterization, and purification of nutraceutical diacylglycerol components from Camellia oil

Abstract

Converting triacylgycerols (TAGs) from edible oils and fats into structured diacylglycerols (DAGs) is meaningful for reducing obesity. Camellia oil, rich in linoleic acid, has the potential to form structured linoleic acid-1,3-diacylglycerol (LA-1,3-DAG) nutrients in the industry. In this research, the physicochemical properties of modified Camellia oil (MCO) by enzymatic esterification were analyzed by Gas Chromatography-Mass Spectrometry (GC-MS), Differential Scanning Calorimetry (DSC), High Performance Liquid Chromatography-Evaporative Light Scattering Detection (HPLC-ELSD), and Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry (UPLC-MS/MS). The relationship between reaction conditions and the DAG compositions is disclosed using multiple factors. It is found that high constituents of DAG increase the melting and crystallization temperature of MCO, lipase Novozym 435 gives the best yield of targeted nutrients (DAG, 1,3-DAG, LA-DAG), and the mixture of lipases, Lipozyme TL IM and Lipozyme RM IM, shows a synergistic effect in the synthetic process of DAG. Subsequently, MCO containing 65.4% DAG, 54.7% LA-DAG, and 47.6% 1,3-DAG content at optimal conditions (2% enzyme dosage, 4 h reaction time, 2.4:1 substrate molar ratio, 25.8% t-butanol as solvent, 60 • C temperature) has been obtained and purified using silica column to obtain the final DAG oil containing 96.1% DAG, 64.7% 1,3-DAG, and 78.4% LA-DAG. High constituents of structured DAG oil rich in LA-1,3-DAG can be obtained by enzymatic esterification for industrial production.

Insight into the medium‐long‐medium structured lipids made from Camellia oil: Composition–structure relationship

“…They can change the configuration of enzymes in active site and lipase lid, respectively. (Huang et al, 2022a(Huang et al, , 2022cZitian et al, 2020). Although Novozym 435 is a lipase that acts as non-1,3 specific, when a great excess of ethanol is used, this lipase acts as 1,3-specific (Muñío et al, 2009).…”

Effect of substrate composition on physicochemical properties of the medium–long–medium structured triacylglycerol

“…Triacylglycerol composition was determined by HPLC (APS80-16T, Aupos Scientific, Berlin, Germany) equipped with an evaporative light-scattering detector (APS80-16E). 25,26 The separation of TAG species was performed using an Ultimate XB-C18 column (particle size 3 μm, 4.6 mm × 150 mm, Welch Materials, Inc., Shanghai, China) and eluted with a binary gradient of acetonitrile containing 0.15% acetic acid (v/v; A) and isopropanol (B) at a flow of 1.0 mL min −1 with the following program: 0-4 min, 0% B; 5-12 min, 10% B; 13-25 min, 10% B; 26-30 min, 30% B; 31-35 min, 30% B; 36-45 min, 80% B; 46-48 min, 80% B; 49-50 min, 0% B; 51-55 min, 0% B. The ELSD conditions were as follows: atomizer temperature, 50 °C; drift pipe temperature, 70 °C; nebulizer gas pressure, 1.05 bar.…”