The electrospinning method provides fabrication of nonwoven mats from nano to micrometers in size with controllable pores. Porous electrospun scaffolds have attracted increasing attention in biomedical applications especially due to their ability to mimic the extracellular matrix. This chapter presents a comprehensive overview of the advancements in the last five years through the design and preparation of porous biobased polymer mats using the electrospinning method for biomedical applications. Firstly, fundamentals of the electrospinning process are presented and followed by a discussion of the possible biomedical applications of micro-and nanostructured porous mats for drug delivery, wound dressing, tissue engineering, and other applications. Various crucial points for limitations and possible future trends are presented.