Fabrication of 3D GelMA Scaffolds Using Agarose Microgel Embedded Printing

Yang, Bíngen; Liu, Tianqi; Gao, Ge; Zhang, Xianglin; Wu, Bin

doi:10.3390/mi13030469

Cited by 19 publications

(21 citation statements)

References 23 publications

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“…A good suspension material should also possess the shear thinning property and a certain yield stress to meet the requirements for embedded bioprinting. [ 32 ] The physically crosslinked SF hydrogel was found to be a good support bath. The shear‐thinning property of SF hydrogel was first validated.…”

Section: Resultsmentioning

confidence: 99%

Embedded Bioprinting of Breast Tumor Cells and Organoids Using Low‐Concentration Collagen‐Based Bioinks

Shi,

Mirza,

Kuss

et al. 2023

Adv Healthcare Materials

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Bioinks for 3D bioprinting of tumor models should not only meet printability requirements but also accurately maintain and support phenotypes of tumor surrounding cells to recapitulate key tumor hallmarks. Collagen is a major extracellular matrix protein for solid tumors, but low viscosity of collagen solution has made 3D bioprinted cancer models challenging. This work produces embedded, bioprinted breast cancer cells and tumor organoid models using low‐concentration collagen I based bioinks. The biocompatible and physically crosslinked silk fibroin hydrogel is used to generate the support bath for the embedded 3D printing. The composition of the collagen I based bioink is optimized with a thermoresponsive hyaluronic acid‐based polymer to maintain the phenotypes of both the noninvasive epithelial and invasive breast cancer cells, as well as cancer‐associated fibroblasts. Mouse breast tumor organoids are bioprinted using optimized collagen bioink to mimic in vivo tumor morphology. A vascularized tumor model is also created using a similar strategy, with significantly enhanced vasculature formation under hypoxia. This study shows the great potential of embedded bioprinted breast tumor models utilizing a low‐concentration collagen‐based bioink for advancing the understanding of tumor cell biology and facilitating drug discovery research.

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Section: Resultsmentioning

confidence: 99%

Embedded Bioprinting of Breast Tumor Cells and Organoids Using Low‐Concentration Collagen‐Based Bioinks

Shi,

Mirza,

Kuss

et al. 2023

Adv Healthcare Materials

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“…However, 3D printing of cryogels using natural polymers, such as gelatin, alginate, collagen, silk, and hyaluronan, has hardly been performed since keeping the shape fidelity of low-viscosity natural polymer inks is challenging during extrusion without cross-linking. Embedded printing in a shear-thinning support bath could be a promising strategy to maintain the shape fidelity of low-viscosity inks which enables the deposition of complex structures. − Through the combination of embedded printing and cryogelation, complex-shaped cryogels can be produced from low-viscosity natural polymers, such as GelMA.…”

Section: Introductionmentioning

confidence: 99%

Embedded 3D Printing of Cryogel-Based Scaffolds

Bilici

Altunbek

Afghah

et al. 2023

ACS Biomater. Sci. Eng.

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Cryogel-based scaffolds have attracted great attention in tissue engineering due to their interconnected macroporous structures. However, three-dimensional (3D) printing of cryogels with a high degree of precision and complexity is a challenge, since the synthesis of cryogels occurs under cryogenic conditions. In this study, we demonstrated the fabrication of cryogel-based scaffolds for the first time by using an embedded printing technique. A photo-cross-linkable gelatin methacryloyl (GelMA)-based ink composition, including alginate and photoinitiator, was printed into a nanoclay-based support bath. The layer-by-layer extruded ink was held in complex and overhanging structures with the help of pre-cross-linking of alginate with Ca 2+ present in the support bath. The printed 3D structures in the support bath were frozen, and then GelMA was cross-linked at a subzero temperature under UV light. The printed and cross-linked structures were successfully recovered from the support bath with an integrated shape complexity. SEM images showed the formation of a 3D printed scaffold where porous GelMA cryogel was integrated between the cross-linked alginate hydrogels. In addition, they showed excellent shape recovery under uniaxial compression cycles of up to 80% strain. In vitro studies showed that the human fibroblast cells attached to the 3D printed scaffold and displayed spread morphology with a high proliferation rate. The results revealed that the embedded 3D printing technique enables the fabrication of cytocompatible cryogel based scaffolds with desired morphology and mechanical behavior using photo-cross-linkable bioink composition. The properties of the cryogels can be modified by varying the GelMA concentration, whereby various shapes of scaffolds can be fabricated to meet the specific requirements of tissue engineering applications.

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“…The mechanical strength conferred by the bioink is necessary for various biofabrication applications . It could be derived from the cross-linking ability , or by adding sacrificial materials. − The sacrificial material provides additional support to the scaffold, as it can be deposited along with the bioink during printing and later could be removed through temperature or UV treatment. Biodegradability becomes an important parameter when designing implants, as the nontoxic degradation process should maintain an equilibrium between the rate of production of cells and the rate of degradation of the biomaterial used for providing the substrate/matrix. , Thus, selecting an appropriate bioink/biomaterial requires careful experimental and physical examination of the contents and their cumulative response during deposition using various techniques.…”

Section: Introductionmentioning

confidence: 99%

The Art of Building Living Tissues: Exploring the Frontiers of Biofabrication with 3D Bioprinting

Verma,

Khanna,

Kumar

et al. 2023

ACS Omega

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The scope of three-dimensional printing is expanding rapidly, with innovative approaches resulting in the evolution of state-of-the-art 3D bioprinting (3DbioP) techniques for solving issues in bioengineering and biopharmaceutical research. The methods and tools in 3DbioP emphasize the extrusion process, bioink formulation, and stability of the bioprinted scaffold. Thus, 3DbioP technology augments 3DP in the biological world by providing technical support to regenerative therapy, drug delivery, bioengineering of prosthetics, and drug kinetics research. Besides the above, drug delivery and dosage control have been achieved using 3D bioprinted microcarriers and capsules. Developing a stable, biocompatible, and versatile bioink is a primary requisite in biofabrication. The 3DbioP research is breaking the technical barriers at a breakneck speed. Numerous techniques and biomaterial advancements have helped to overcome current 3DbioP issues related to printability, stability, and bioink formulation. Therefore, this Review aims to provide an insight into the technical challenges of bioprinting, novel biomaterials for bioink formulation, and recently developed 3D bioprinting methods driving future applications in biofabrication research.

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Fabrication of 3D GelMA Scaffolds Using Agarose Microgel Embedded Printing

Cited by 19 publications

References 23 publications

Embedded Bioprinting of Breast Tumor Cells and Organoids Using Low‐Concentration Collagen‐Based Bioinks

Embedded Bioprinting of Breast Tumor Cells and Organoids Using Low‐Concentration Collagen‐Based Bioinks

Embedded 3D Printing of Cryogel-Based Scaffolds

The Art of Building Living Tissues: Exploring the Frontiers of Biofabrication with 3D Bioprinting

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