Fabrication of 3D high aspect ratio PDMS microfluidic networks with a hybrid stamp

Kung, Yu Chun; Huang, Kuan-Chieh; Fan, Yuanyuan; Chiou, Pei-Yu

doi:10.1039/c4lc01211a

Cited by 50 publications

(28 citation statements)

References 33 publications

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“…In addition, PS nanoparticles with negative charge can also be used for calibration of microfluidic devices because of their uniform and controllable particle size. In microfluidic applications such as flow cytometry, cell sorting, and imaging, it is a key capability to three-dimensionally focus randomly distributed particles into a single flow [14,15].Polymers 2020, 12, 574 3 of 11…”

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confidence: 99%

Designing Micro Bulge Structure with Uniform PS Microspheres for Boosted Dielectric Hydrophobic Blend Films

et al. 2020

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In this paper, homogeneous polystyrene (PS) microspheres with controllable sizes of 40 nm, 80 nm, and 120 nm were synthesized by controlling the temperature of solvothermal method. In order to explore the effect of PS microspheres on dielectric-hydrophobic properties of the composite films, the composite films containing polystyrene, Polydimethylsiloxane, and P(VDF-TrFE) with high dielectric and hydrophobicity were successfully prepared by a simple and feasible solution blending method. The dielectric constant and hydrophobicity of composite films were boosted by increasing the mass fraction of PS content and decreasing the size of PS due to the enhanced interfacial polarization and the uniform surface micro bulge structure. Meanwhile, the composite films maintain a low loss tangent. Typically, the dielectric constant with 5 wt.% 40 nm PS reached to 29 at 100Hz, which is 4 times that of PDMS/P(VDF-TrFE) (mass ratio: 2/3). Otherwise, the largest the contact angle of 126° in the same composition was remarkably larger than the pure PDMS/P(VDF-TrFE) (110°). These improved properties have more potential applications in the electric wetting devices.

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confidence: 99%

Designing Micro Bulge Structure with Uniform PS Microspheres for Boosted Dielectric Hydrophobic Blend Films

et al. 2020

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“…The cured PDMS replica can be bonded with another flat layer of PDMS, glass or other materials to form a closed system. Based on soft lithography, Kung et al 70 demonstrated a novel method for fabricating 3D high aspect ratio PDMS microfluidic networks with a hybrid stamp. Figure 1 shows the schematic of fabrication process flow.…”

Section: Microfluidic Platforms For Biomarker Detectionmentioning

confidence: 99%

Biomarker detection for disease diagnosis using cost-effective microfluidic platforms

Sanjay

Dou

et al. 2015

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Early and timely detection of disease biomarkers can prevent the spread of infectious diseases, and drastically decrease the death rate of people suffering from different diseases such as cancer and infectious diseases. Because conventional diagnostic methods have limited application in low-resource settings due to the use of bulky and expensive instrumentation, simple and low-cost point-of-care diagnostic devices for timely and early biomarker diagnosis, is the need of the hour, especially in rural areas and developing nations. The microfluidics technology possesses remarkable features for simple, low-cost, and rapid disease diagnosis. There have been significant advances in the development of microfluidic platforms for diseases biomarker detection. This article reviews recent advances in biomarker detection using cost-effective microfluidic devices for disease diagnosis, with the emphasis on infectious disease and cancer diagnosis in low-resource settings. This review first introduces different microfluidic platforms (e.g. polymer and paper-based microfluidics) used for disease diagnosis, with a brief description of their common fabrication techniques. Then, it highlights various detection strategies for disease biomarker detection using microfluidic platforms, including colorimetric, fluorescence, chemiluminescence, electrochemiluminescence (ECL), and electrochemical detection. Finally, it discusses the current limitations of microfluidics devices for disease biomarker detection and the future perspectives.

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“…Dielectrophoresis (DEP) has attracted much attention as a manipulation technique for cells [ 16 , 17 , 18 , 19 ]. DEP is based on the interaction between a non-uniform electric field and the polarization charge on the surface of cells.…”

Section: Introductionmentioning

confidence: 99%

Label-Free Rapid Separation and Enrichment of Bone Marrow-Derived Mesenchymal Stem Cells from a Heterogeneous Cell Mixture Using a Dielectrophoresis Device

Yoshioka

Ohsugi

Yoshitomi

et al. 2018

Sensors

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Bone marrow-derived mesenchymal stem cells (BMSCs) are an important cell resource for stem cell-based therapy, which are generally isolated and enriched by the density-gradient method based on cell size and density after collection of tissue samples. Since this method has limitations with regards to purity and repeatability, development of alternative label-free methods for BMSC separation is desired. In the present study, rapid label-free separation and enrichment of BMSCs from a heterogeneous cell mixture with bone marrow-derived promyelocytes was successfully achieved using a dielectrophoresis (DEP) device comprising saw-shaped electrodes. Upon application of an electric field, HL-60 cells as models of promyelocytes aggregated and floated between the saw-shaped electrodes, while UE7T-13 cells as models of BMSCs were effectively captured on the tips of the saw-shaped electrodes. After washing out the HL-60 cells from the device selectively, the purity of the UE7T-13 cells was increased from 33% to 83.5% within 5 min. Although further experiments and optimization are required, these results show the potential of the DEP device as a label-free rapid cell isolation system yielding high purity for rare and precious cells such as BMSCs.

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Fabrication of 3D high aspect ratio PDMS microfluidic networks with a hybrid stamp

Abstract: We report a novel methodology for fabricating large-area, multilayer, thin-film, high aspect ratio, 3D microfluidic structures with through-layer vias and open channels that can be bonded between hard substrates.

Cited by 50 publications

References 33 publications

Designing Micro Bulge Structure with Uniform PS Microspheres for Boosted Dielectric Hydrophobic Blend Films

Designing Micro Bulge Structure with Uniform PS Microspheres for Boosted Dielectric Hydrophobic Blend Films

Biomarker detection for disease diagnosis using cost-effective microfluidic platforms

Label-Free Rapid Separation and Enrichment of Bone Marrow-Derived Mesenchymal Stem Cells from a Heterogeneous Cell Mixture Using a Dielectrophoresis Device

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