Fabrication of a biofuel cell improved by the π-conjugated electron pathway effect induced from a new enzyme catalyst employing terephthalaldehyde

Chung, Yongjin; Hyun, Kyu Hwan; Kwon, Yongchai

doi:10.1039/c5nr06703k

Cited by 62 publications

(33 citation statements)

References 41 publications

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“…This downshift was attributed to the reduction of O 2 (O 2 +2H + +2e − → H 2 O 2 ). 29,30 Supplementary Figures S7a-c represent the CV curves of the catalytic structures measured in N 2 state with 10 mM glucose. In Supplementary Figure S7, the CV curves remained unchanged irrespective of the glucose concentration.…”

Section: Resultsmentioning

confidence: 99%

A new biocatalyst employing pyrenecarboxaldehyde as an anodic catalyst for enhancing the performance and stability of an enzymatic biofuel cell

2017

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A new enzyme catalyst consisting of pyrenecarboxaldehyde (PCA) and glucose oxidase (GOx) immobilized on polyethyleneimine (PEI) and a carbon nanotube supporter (CNT/PEI/[PCA/GOx]) is suggested, and the performance and stability of an enzymatic biofuel cell (EBC) using the new catalyst are evaluated. Using PCA, the amount of immobilized GOx increases (3.3 U mg − 1 ) and the electron transfer rate constant of the CNT/PEI/[PCA/GOx] is promoted (11.51 s − 1 ). Also, the catalyst induces excellent EBC performance (maximum power density (MPD) of 2.1 mW cm − 2 ), long-lasting stability (maintenance of 93% of the initial MPD after 4 weeks) and superior catalytic activity (flavin adenine dinucleotide redox reaction rate of 0.62 mA cm − 2 and Michaelis-Menten constant of 0.99 mM). These characteristics are ascribed to effects of (i) electron collection due to hydrophobic interactions, (ii) electron transfer pathways due to π-conjugated bonds and (iii) enzyme stabilization due to π-hydrogen bonds that are newly induced by the PCA/GOx composite. The existence of such positive interactions is properly verified using X-ray photoelectron spectroscopy and enzyme activity measurements. NPG Asia Materials (2017) 9, e386; doi:10.1038/am.2017.75; published online 2 June 2017 INTRODUCTIONThe demand for clean electrical energy is increasing as a result of weather changes caused by the greenhouse effect and depletion of fossil fuels. 1 To meet this demand, related research and development efforts are being eagerly pursued. As one of the efforts, enzymatic biofuel cells (EBCs) that convert bioenergy into electrical energy are being considered. 2,3 In particular, EBC systems employing glucose oxidase (GOx) as a biocatalyst have emerged because of their advantageous properties, such as biocompatibility, excellent selectivity toward specific substrates and strong activity near neutral pH and room temperature. 2 In addition, because EBC systems use human body-friendly fuels, such as glucose, glycerol, water and oxygen, for electricity generation, they can be embedded as power generators for the operation of artificial internal organs, such as artificial hearts and brains, insulin pumps or bone stimulators. [4][5][6][7][8] However, in spite of such promising facets of EBC systems, the commercialization of EBCs using GOx has been limited because of their low EBC performance and poor durability. 9 The disadvantages are attributed to the low immobilization ratio of GOx molecules, slow GOx-related reaction rate, low GOx activity and easy GOx denaturation. Of them, low GOx immobilization has been considered the main problem.

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Section: Resultsmentioning

confidence: 99%

A new biocatalyst employing pyrenecarboxaldehyde as an anodic catalyst for enhancing the performance and stability of an enzymatic biofuel cell

2017

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“…촉매의 보관 안정성이 낮을 수 있으므로, 이에 GA 또는 TPA를 가하 여, GOx의 효소간 상호결합과 GOx-PEI간의 화학결합을 유도하였다 [9]. 물리적 흡착만으로 제조된 촉매(GOx/PEI/CNT)는 Hyun 등에 의하 여 XPS, TEM, Electrophoretic light scattering spectrophotometer (Zeta-potential)으로 검증되었으며, 안정적으로 최대 담지가능한 GOx의 양은 CNT 2 mg당 2 mg으로 알려져 있다 [10].…”

Section: 결과 및 토론unclassified

A Study on Performance Improvement of Glucose Sensor Adopting a Catalyst Using New Cross Liker

Chung¹,

Kwon²

2015

Korean Chemical Engineering Research

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− In this study, we synthesized a new biocatalyst consisting of glucose oxidase (GOx), polyethyleneimine (PEI) and carbon nanotube (CNT) with addition of terephthalaldehyde (TPA) (TPA/GOx/PEI/CNT) for fabrication of glucose sensor that shows improved sensing ability and stability compared with that using other biocatalysts. Main bonding of the new TPA/GOx/PEI/CNT catalyst is formed by Aldol condensation reaction of functional end groups between GOx/ PEI and TPA. Such formed bonding structure promotes oxidation reaction of glucose. Catalytic activity of TPA/GOx/PEI/CNT is evaluated quantitatively by electrochemical measurements. As a result of that, large sensitivity value of 41 µAcm -2 mM -1 is gained. Regarding biosensor stability of TPA/GOx/PEI/CNT catalyst, covalent bonding formed between GOx/PEI and TPA prevents GOx molecules from becoming leaching-out and contributes improvement in biosensor stability. With estimation of the biosensor stability, it is found that the TPA/GOx/PEI/CNT catalyst keeps 94.6% of its initial activity even after three weeks.

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“…This result proved that PEI could effectively improve the protein shelf life [7]. PEI is a conductive polymer and serves as a pathway for electron transfer from the flavin adenine dinucleotide (FAD) of GOx to the carbon material substrate [8]. In 2014, Xu et al performed research fabricating a Pd-PEI/GO/GC electrode using a layer-by-layer method.…”

Section: Introductionmentioning

confidence: 99%

Polyethyleneimine Modified Carbon Cloth Anode for Self-Pumping Enzymatic Glucose Biofuel Cell

Huang

Duong

Wang

et al. 2018

Journal of Renewable Energy

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This paper proposes a simplified process that immobilizes enzymes onto carbon cloth electrodes to increase biofuel cell functionality. Polyethyleneimine (PEI) is used to modify carbon cloth electrodes to reduce the processing time and increase selfpumping enzymatic glucose biofuel cell (self-pumping EGBC) electricity. PEI is usually used in biochemical engineering gene transfection as GOx support to enhance enzyme immobilization. PEI is a good candidate for increasing enzymatic biofuel cell (EBC) redox current. PEI and GOx have been successfully immobilized onto carbon cloth electrodes through FT-IR analysis. A UV/Vis spectrophotometer was used to investigate the best PEI support concentration. PEI was proven to improve redox current by cyclic voltammetry analysis. The results show that the GOx/PEI electrode has excellent hydrophilicity on the GOx/PEI electrode surface using contact angle measurement. The optical and electrochemical analysis result shows that GOx/PEI was successfully immobilized onto carbon cloth electrodes. Experimental analysis showed that self-pumping EGBC achieved a power output of 0.609 mW/cm 2 (126.9 mW/cm 3 ). PEI contributes to the shortening of the process from a few hours to 5-10 minutes and enhances GOx fuel cell performance.

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Fabrication of a biofuel cell improved by the π-conjugated electron pathway effect induced from a new enzyme catalyst employing terephthalaldehyde

Cited by 62 publications

References 41 publications

A new biocatalyst employing pyrenecarboxaldehyde as an anodic catalyst for enhancing the performance and stability of an enzymatic biofuel cell

A new biocatalyst employing pyrenecarboxaldehyde as an anodic catalyst for enhancing the performance and stability of an enzymatic biofuel cell

A Study on Performance Improvement of Glucose Sensor Adopting a Catalyst Using New Cross Liker

Polyethyleneimine Modified Carbon Cloth Anode for Self-Pumping Enzymatic Glucose Biofuel Cell

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