Fabrication of a Cartilage Patch by Fusing Hydrogel-Derived Cell Aggregates onto Electrospun Film

Zhang, Jiabin; Yun, Seonho; Du, Yuguang; Zannettino, Andrew C.W.; Zhang, Hu

doi:10.1089/ten.tea.2019.0318

Cited by 18 publications

(15 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For this, they were also explored as 3D models for cancer research. The use of 3D scaffolding models based on scaffolding expands the range of options available to researchers [140][141][142][143][144][145][146][147][148].…”

Section: Hydrogelsmentioning

confidence: 99%

“…Matrigel ® -Widely available -Frequently used in cancer research [151,[173][174][175][176] -Unknown and uncontrollable amount growth factors [24] -Lack of control over its exact composition -Variable from batch to batch [178] -Difficulties of handling when it is in a refrigerated liquid state [104] Based on Collagen -Good adhesion and cell migration support [145][146][147][148]156] -Biocompatibility, mechanical strength, degradability, and limited immunogenicity [157,158] -The most widely used tissue engineering and in tumor culture [11,152,158,[199][200][201][202] -Cell signaling patterns [140][141][142][143][144]203] -Animal origin can potentially transmit pathogens [204] -Biodegradable [159] Hyaluronic acid -Provide hydration and resistance for cellular mechanisms [33,35,39] -Biodegradable, non-immunogenic, non-inflammatory [205] -Hydrodynamic and swelling [33,35] -Animal origin can potentially transmit pathogens [204].…”

Section: Hydrogel Advantage Disadvantagesmentioning

confidence: 99%

See 1 more Smart Citation

3D Cell Culture Systems: Tumor Application, Advantages, and Disadvantages

Habanjar

Diab‐Assaf

Caldefie-Chézet

et al. 2021

IJMS

261

157

View full text Add to dashboard Cite

The traditional two-dimensional (2D) in vitro cell culture system (on a flat support) has long been used in cancer research. However, this system cannot be fully translated into clinical trials to ideally represent physiological conditions. This culture cannot mimic the natural tumor microenvironment due to the lack of cellular communication (cell-cell) and interaction (cell-cell and cell-matrix). To overcome these limitations, three-dimensional (3D) culture systems are increasingly developed in research and have become essential for tumor research, tissue engineering, and basic biology research. 3D culture has received much attention in the field of biomedicine due to its ability to mimic tissue structure and function. The 3D matrix presents a highly dynamic framework where its components are deposited, degraded, or modified to delineate functions and provide a platform where cells attach to perform their specific functions, including adhesion, proliferation, communication, and apoptosis. So far, various types of models belong to this culture: either the culture based on natural or synthetic adherent matrices used to design 3D scaffolds as biomaterials to form a 3D matrix or based on non-adherent and/or matrix-free matrices to form the spheroids. In this review, we first summarize a comparison between 2D and 3D cultures. Then, we focus on the different components of the natural extracellular matrix that can be used as supports in 3D culture. Then we detail different types of natural supports such as matrigel, hydrogels, hard supports, and different synthetic strategies of 3D matrices such as lyophilization, electrospiding, stereolithography, microfluid by citing the advantages and disadvantages of each of them. Finally, we summarize the different methods of generating normal and tumor spheroids, citing their respective advantages and disadvantages in order to obtain an ideal 3D model (matrix) that retains the following characteristics: better biocompatibility, good mechanical properties corresponding to the tumor tissue, degradability, controllable microstructure and chemical components like the tumor tissue, favorable nutrient exchange and easy separation of the cells from the matrix.

show abstract

Section: Hydrogelsmentioning

confidence: 99%

Section: Hydrogel Advantage Disadvantagesmentioning

confidence: 99%

3D Cell Culture Systems: Tumor Application, Advantages, and Disadvantages

Habanjar

Diab‐Assaf

Caldefie-Chézet

et al. 2021

IJMS

261

157

View full text Add to dashboard Cite

show abstract

“…The structure of multi-empty cells mass formed in the hydrogels group was more compact than that in the control group. [ 77 , 78 ] HA/PEG-p (HPMAm-lac) - Slow release In vitro & vivo Mice Hydrogels are able to inhibition the inflammatory process in a mouse model of OA through the controlled and sustained release of HA over a time period that goes from 30 to 70 days in vitro; For OA caused by injection of collagenase, the hydrogels demonstrated the ability to restore, to some extent, bone remineralization, proteoglycan production, levels of Sox‐9 and Runx‐2. Furthermore, the downregulation of proinflammatory mediators, such as TNF‐α, NFκB, and RANKL and proinflammatory cytokines was observed.…”

Section: Different Drug-delivery Systems For Intra-articular Injectio...mentioning

confidence: 99%

“…Temperature-sensitive hydrogels have always been favored because they are easier to load with drugs or cells and possess more convenient in situ formation properties than other hydrogels. 66 , 67 Thermosensitive hydrogels, such as chitosan with β-glycerol phosphate, 72 glycol chitosan, 73 PEG-grafted polyalanine or poly(lactide-co-glycolide), 74–76 poly(N-isopropylacrylamide-co-acrylic acid) (p(NIPAAm-AA)), 77 , 78 poly(ethyleneglycol)-(p(HPMAm-lac)-PEG), 79 poloxamers, 80 poly(d,l-lactide)-poly(ethylene glycol)-poly(d,l-lactide) (PLEL), 81 , 82 hyaluronic acid-chitosan-poly(N-isopropylacrylamide), 83 and amphiphilic poly(organophosphazene), 84 have been increasingly applied in the treatment of OA. A previous study demonstrated that temperature-sensitive PNIPAM (poly(N-isopropylacrylamide)) hydrogels 85 could regulate drug release according to the joint temperature.…”

Section: Different Drug-delivery Systems For Intra-articular Injectio...mentioning

confidence: 99%

Knee Osteoarthritis Therapy: Recent Advances in Intra-Articular Drug Delivery Systems

Ma¹,

Zheng²,

Li³

et al. 2022

DDDT

View full text Add to dashboard Cite

Drug delivery for osteoarthritis (OA) treatment is a continuous challenge because of their poor bioavailability and rapid clearance in joints. Intra-articular (IA) drug delivery is a common strategy and its therapeutic effects depend mainly on the efficacy of the drug-delivery system used for OA therapy. Different types of IA drug-delivery systems, such as microspheres, nanoparticles, and hydrogels, have been rapidly developed over the past decade to improve their therapeutic effects. With the continuous advancement in OA mechanism research, new drugs targeting specific cell/signaling pathways in OA are rapidly evolving and effective drug delivery is critical for treating OA. In this review, recent advances in various IA drug-delivery systems for OA treatment, OA targeted strategies, and related signaling pathways in OA treatment are summarized and analyzed based on current publications.

show abstract

“…84,[96][97][98] There have been a variety of studies showing that cellular functions are enhanced in cell aggregates compared to single-cell suspensions, such as the enhanced immunomodulatory potential and multi-lineage differentiation of MSC aggregates and the increased production of albumin and urea in hepatocyte aggregates. 22,[99][100][101][102][103][104][105][106] Moreover, cell aggregates also show improved cell viability and survival rate after implantation in vivo. [8][9][10][11] Cell aggregates can be generated by different methods, [107][108][109][110] such as cell culture on low attachment plates or in hanging drops, as well as substratebased and technology-assisted methods, amongst which 3D scaffold/hydrogel-based approaches are of high interest since cells can form cell aggregates in situ after seeding inside the 3D scaffolds/hydrogels.…”

Section: Cell Aggregate-based Approachmentioning

confidence: 99%

Stem cell therapy and tissue engineering strategies using cell aggregates and decellularized scaffolds for the rescue of liver failure

et al. 2021

Self Cite

View full text Add to dashboard Cite

Liver failure is a lethal condition with hepatocellular dysfunction, and liver transplantation is presently the only effective treatment. However, due to the limited availability of donors and the potential immune rejection, novel therapeutic strategies are actively sought to restore the normal hepatic architectures and functions, especially for livers with inherited metabolic dysfunctions or chronic diseases. Although the conventional cell therapy has shown promising results, the direct infusion of hepatocytes is hampered by limited hepatocyte sources, poor cell viability, and engraftment. Hence, this review mainly highlights the role of stem cells and progenitors as the alternative cell source and summarizes the potential approaches based on tissue engineering to improve the delivery efficiency of cells. Particularly, the underlying mechanisms for cell therapy using stem cells and progenitors are discussed in two main aspects: paracrine effect and cell differentiation. Moreover, tissue-engineering approaches using cell aggregates and decellularized liver scaffolds for bioengineering of functional hepatic constructs are discussed and compared in terms of the potential to replicate liver physiological structures. In the end, a potentially effective strategy combining the premium advantages of stem cell aggregates and decellularized liver scaffolds is proposed as the future direction of liver tissue engineering and regeneration.

show abstract

Fabrication of a Cartilage Patch by Fusing Hydrogel-Derived Cell Aggregates onto Electrospun Film

Cited by 18 publications

References 35 publications

3D Cell Culture Systems: Tumor Application, Advantages, and Disadvantages

3D Cell Culture Systems: Tumor Application, Advantages, and Disadvantages

Knee Osteoarthritis Therapy: Recent Advances in Intra-Articular Drug Delivery Systems

Stem cell therapy and tissue engineering strategies using cell aggregates and decellularized scaffolds for the rescue of liver failure

Contact Info

Product

Resources

About