Fabrication of a Chitosan‐Coated Magnetic Nanobiocatalyst for Starch Hydrolysis

Jain, Manisha; Sebatini, Mariya; Sharmila, G.; Chandrasekaran, Muthukumaran; Baskar, G.; Tamilarasan, K.

doi:10.1002/ceat.201400493

Cited by 18 publications

(1 citation statement)

References 41 publications

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“…The technique of co-precipitation was used to prepare magnetic nanoparticles according to [19,20]. Below aerobic circumstances, the Fe2+ can be readily oxidized to Fe3+.…”

Section: Methods 221 Preparing Of Mnpsmentioning

confidence: 99%

Enhanced biological activity of Candida rugosa lipase via immobilization on magnetic nanoparticles

et al. 2020

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Magnetic nanoparticles Fe3O4 were synthesized by the co-precipitation method. Magnetic nanoparticles were activated and modified with (3-aminopropyl) triethoxysilane (APTES) and glutaraldehyde (GA), respectively. Then, these nanoparticles were characterized by transmission electron microscope and Fourier transform infrared. Candida rugosa lipase (CRL) was successfully immobilized on GAMNPs by the chemical method via forming a covalent bond between a functional group on an enzyme protein molecule and a reactive group on the surface of solid support by chemical interaction. CRL-Fe3O4 displayed an elevate recovery rate of upward to 1348%, which was a 13-fold improvement in its free one. Compared to a free enzyme, the pH and thermal properties of the immobilized lipase were also increased. This study clearly indicates that GAMNPs could be deemed good support for the immobilization of enzymes.

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“…The technique of co-precipitation was used to prepare magnetic nanoparticles according to [19,20]. Below aerobic circumstances, the Fe2+ can be readily oxidized to Fe3+.…”

Section: Methods 221 Preparing Of Mnpsmentioning

confidence: 99%