Fabrication of a Detection Platform with Boronic-Acid-Containing Zwitterionic Polymer Brush

Abstract

Development of technologies for biomedical detection platform is critical to meet the global challenges of various disease diagnoses, especially for point-of-use applications. Because of its natural simplicity, effectiveness, and easy repeatability, random covalent-binding technique is widely adopted in antibody immobilization. However, its antigen-binding capacity is relatively low when compared to site-specific immobilization of antibody. Herein, we report that a detection platform modified with boronic acid (BA)-containing sulfobetaine-based polymer brush. Mainly because of the advantage of oriented immobilization of antibody endowed with BA-containing three-dimensional polymer brush architecture, the platform had a high antigen-binding capacity. Notably, nonspecific protein adsorption was also suppressed by the zwitterionic pendants, thus greatly enhanced signal-to-noise (S/N) values for antigen recognition. Furthermore, antibodies captured by BA pendants could be released in dissociation media. This new platform is promising for potential applications in immunoassays.

Metal–Organic Frameworks with Boronic Acid Suspended and Their Implication for cis‐Diol Moieties Binding

“…The C1s XPS spectra could provide further information about the introduced functional groups on MIL‐100‐B. For a BBDC/BTC ratio of 1:4, the deconvoluted C1s peak (Figure d) consists of four peaks, which could be assigned to C in C‐B (283.9 eV), C‐C/C=C (284.6 eV), C‐O (285.7 eV), and O‐C=O (288.5 eV), respectively . Similar peaks could be observed in the deconvoluted C1s peak of MOFs with BBDC/BTC feed ratios of 1:2.3 (Figure e) and 1:1.5 (Figure f).…”

Key steps towards the oriented immobilization of antibodies using boronic acids

“…1). [8][9][10][11][12][13][14][15][16][17][18] An overview of these articles is given in Table 1. 6 Oriented Ab immobilization using boronic acids (through the same reaction) was reported for the first time in 2009 by Lin et al, 7 and has appeared in another eleven papers since then.…”

Comparative Assessment of Affinity-Based Techniques for Oriented Antibody Immobilization towards Immunosensor Performance Optimization

“…The rearrangement of boronate esters in which the esters dissociate by hydrolysis to yield free boronic acids and diols is greatly affected by both pH as well as the presence of other competing saccharides [27]. For example, it has been shown that at alkaline pH values high levels of antibody dissociation are observed upon incubation with sugar-containing media [28]. In the case of immunosensors, the buffering systems usually employed are in the physiological pH range, while exposure to complex sample matrices is highly likely and depends on the application.…”