Fabrication of a Flexible Amperometric Glucose Sensor Using Additive Processes

Abstract

This study details the use of printing and other additive processes to fabricate a novel amperometric glucose sensor. The sensor was fabricated using a Au coated 12.7 μm thick polyimide substrate as a starting material, where micro-contact printing, electrochemical plating, chloridization, electrohydrodynamic jet (e-jet) printing, and spin coating were used to pattern, deposit, chloridize, print, and coat functional materials, respectively. We have found that e-jet printing was effective for the deposition and patterning of glucose oxidase inks with lateral feature sizes between ~5 to 1000 μm in width, and that the glucose oxidase was still active after printing. The thickness of the permselective layer was optimized to obtain a linear response for glucose concentrations up to 32 mM and no response to acetaminophen, a common interfering compound, was observed. The use of such thin polyimide substrates allow wrapping of the sensors around catheters with high radius of curvature ~250 μm, where additive and microfabrication methods may allow significant cost reductions.

Real-Time Monitoring of Cellular Bioenergetics with a Multianalyte Screen-Printed Electrode

“…This modification strategy contrasts with a recently published study where cross-linker was added to the enzyme ink prior to printing, and the diffusion layer was applied via spin-coating. 25 …”

Structural and optical characterization of CuInS2 quantum dots synthesized by microwave-assisted continuous flow methods

“…The electrohydrodynamic (e-jet) printer has been described in detail elsewhere. (Du et al 2015) In brief, a glass needle with a 30-lm tip diameter (World Precision Industries) was sputter coated with 20 nm of Au/Pd to make the outer surface conductive. The needle was dipped in 1H,1H,2H,2H-perfluorodecanethiol (Sigma-Aldrich) for 2 min to make the outside of the needle hydrophobic.…”

Amorphous In-Ga-Zn-O thin-film transistors fabricated by microcontact printing

“…4 Low-cost alternative patterning methods, such as soft lithography, may be of great benefit to large-scale manufacturing, especially for roll-to-roll processes. [14][15][16] Microcontact printing (lCP) is a soft lithography approach where self-assembled monolayers (SAMs) are transferred from a patterned stamp onto a surface, and the SAMs are used as resists to prevent chemical etching, 14 and as initiators or surface energy modulators to guide material deposition. 17 lCP has been shown to be a convenient, low-cost method to pattern transparent oxide films, including indium tin oxide (ITO) and indium zinc oxide, with minimum feature sizes $2 lm, which is below the critical dimensions required for displays.…”