Fabrication of a microfluidic cell analyzer in a microchannel using impedance spectroscopy

Gawad, Shady; Henschkel, M.; Leung-Ki, Y.; Iuzzolino, Ricardo; Schild, Laurent; Lerch, Philippe; Renaud, Philippe

doi:10.1109/mmb.2000.893791

Cited by 14 publications

(8 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As for dielectrophoretic cell manipulation the group of Prof. Fuhr has demonstrated many impressive actuators such as the AC octode field cages, which allow precise particle positioning 2 as well as cell alignment and sorting capability. 3 Three other designs of micromachined particle counters [4][5][6][7] have been presented so far, starting with the one presented by Larsen, 5 but no measurements with a standard physiological saline solution of conductivity s sol = 1.6 S m 21 and no evidence of cell differentiation have been published to date. Dielectrophoresis and electrorotation methods to characterise the cell's electrical parameters have also been demonstrated lately but even automated microsystems take several minutes per cell.…”

Section: Introductionmentioning

confidence: 99%

Micromachined impedance spectroscopy flow cytometer for cell analysis and particle sizing

2001

Self Cite

View full text Add to dashboard Cite

A new cytological tool, based on the microCoulter particle counter (microCPC) principle, aimed at diagnostic applications for cell counting and separation in haematology, oncology or toxicology is described. The device measures the spectral impedance of individual cells or particles and allows screening rates over 100 samples s(-1) on a single-cell basis. This analyzer is intended to drive a sorting actuator producing a subsequent cell separation. Size reduction and integration of functions are essential in achieving precise measurements and high throughput. 3D finite element simulations are presented to compare various electrode geometries and their influence on cell parameters estimation. The device is based on a glass-polyimide microfluidic chip with integrated channels and electrodes microfabricated at the length scale of the particles to be investigated (1-20 microm). A laminar liquid flow carries the suspended particles through the measurement area. Each particle's impedance signal is recorded by a differential pair of microelectrodes using the cell surrounding media as a reference. The micromachined chip and processing electronic circuit allow simultaneous impedance measurements at multiple frequencies, ranging from 100 kHz to 15 MHz. In this paper, we describe the microfabrication and characterisation of an on-chip flow-cytometer as the first building block of a complete cell-sorting device. We then discuss the signal conditioning technique and finally impedance measurements of cells and particles of different sizes and types to demonstrate the differentiation of subpopulations in a mixed sample.

show abstract

Section: Introductionmentioning

confidence: 99%

Micromachined impedance spectroscopy flow cytometer for cell analysis and particle sizing

2001

Self Cite

View full text Add to dashboard Cite

show abstract

“…For many medical and scientific applications, the diameter of the Coulter aperture ranges from approximately 30 to 200 m. Because of the small dimensions involved, integrated circuit technology has been used to fabricate the Coulter aperture [2,3] and even complete integrated Coulter counters without [4][5][6] and with hydrodynamic focussing [3]. However, these devices still use a fixed Coulter aperture, thereby having to make the trade-off between sensitiv-ity and the chance of clogging: the smaller the aperture the higher the sensitivity, but the larger the chance of clogging.…”

Section: Introductionmentioning

confidence: 99%

Integrated Coulter counter based on 2-dimensional liquid aperture control

Nieuwenhuis

Köhl

Bastemeijer

et al. 2004

Sensors and Actuators B: Chemical

View full text Add to dashboard Cite

“…Systems and DNA Detection Devices. These devices include, but are not limited to are devices for cell sampling(75), cell trapping and cell sorting devices(59,(76)(77)(78)(79), flow cytometers(67,80,81), devices for cell treatment: cell lysis, poration/gene transfection, and cell fusion devices(82). The first generation of microfluidicsbased analytical devices [Lab-on-a-Chip (64)] have been designed and are already functional.…”

mentioning

confidence: 99%