Fabrication of a novel disposable glucose biosensor using an electrochemically reduced graphene oxide–glucose oxidase biocomposite

Kathiresan, Vijayaraj; Hong, Suck Won; Jin, Sung Ho; Chang, Seung‐Cheol; Park, Deog-Su

doi:10.1039/c6ay02032a

Cited by 25 publications

(11 citation statements)

References 53 publications

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“…Their combination with electrochemical detection techniques offer to develop selective and sensitive detection systems which provide to obtain fast and reliable sensor response. Furthermore, their robust structure allow the modification of its graphite surface with different nanomaterials such as carbon nanotubes , graphene and graphene oxide dendrimers and different forms of nanoparticles .…”

Section: Introductionmentioning

confidence: 99%

Hydroxyapatite Nanoparticles Modified Graphite Electrodes for Electrochemical DNA Detection

Erdem

Çongur

2017

Electroanalysis

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Hydroxyapatite nanoparticles (HaNP) modified pencil graphite electrodes (PGEs) were developed for the first time in the literature, and accordingly they were applied for electrochemical monitoring of sequenceselective DNA hybridization. The experimental conditions for HaNP modification of PGE, and DNA hybridization related to Hepatitis B Virus (HBV) DNA sequence were optimized. The microscopic and electrochemical characterization of HaNP-PGE in contrast to the unmodified one was utilized. Under optimized experimental conditions, the selectivity of HBV DNA probe immobilized biosensor was tested against to non-complementary (NC), mismatch (MM) sequences and the mixture of target:NC (1 : 1) or target: MM (1 : 1).

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Section: Introductionmentioning

confidence: 99%

Hydroxyapatite Nanoparticles Modified Graphite Electrodes for Electrochemical DNA Detection

Erdem

Çongur

2017

Electroanalysis

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“…For both sets of preliminary stability data, the biosensor demonstrated good short-term stability; however, it must be considered that this is not representative of a comprehensive stability study. Further investigation is necessary to understand the sensor behaviour over time and to determine the long-term storage capabilities [43,44]. In this study, as a potential proof of concept, the ultra-microelectrode response was measured in fetal bovine serum to evaluate the sensor's performance in an un-diluted biological serum.…”

Section: Selectivity Short-term Stability and Serum Analysismentioning

confidence: 99%

On-Chip Glucose Detection Based on Glucose Oxidase Immobilized on a Platinum-Modified, Gold Microband Electrode

et al. 2021

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We report the microfabrication and characterization of gold microband electrodes on silicon using standard microfabrication methods, i.e., lithography and etching techniques. A two-step electrodeposition process was carried out using the on-chip platinum reference and gold counter electrodes, thus incorporating glucose oxidase onto a platinum-modified, gold microband electrode with an o-phenylenediamine and ß-cyclodextrin mixture. The as-fabricated electrodes were studied using optical microscopy, scanning electron microscopy, and atomic force microscopy. The two-step electrodeposition process was conducted in low sample volumes (50 µL) of both solutions required for biosensor construction. Cyclic voltammetry and electrochemical impedance spectroscopy were utilised for electrochemical characterization at each stage of the deposition process. The enzymatic-based microband biosensor demonstrated a linear response to glucose from 2.5–15 mM, using both linear sweep voltammetry and chronoamperometric measurements in buffer-based solutions. The biosensor performance was examined in 30 µL volumes of fetal bovine serum. Whilst a reduction in the sensor sensitivity was evident within 100% serum samples (compared to buffer media), the sensor demonstrated linear glucose detection with increasing glucose concentrations (5–17 mM).

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“…No noticeable change in current was observed in the presence of interfering species indicating that the biosensor is selective to glucose measurements. A number of investigations examining glucose levels in spiked serum samples have been undertaken in serum solutions diluted in PBS (Mehmeti et al, 2017) (Vijayaraj et al, 2016), in this study the electrode response was measured in 100% fetal bovine serum to evaluate the sensor performance in an un-diluted biological serum. The fetal bovine serum was spiked with known concentrations of glucose and a chronoamperometric response was measured for 70 seconds at 0.25 V. A linear response was observed in the range of 2 -12 mM (Figure 5 3).…”

Section: Interference and Foetal Bovine Serum Measurementsmentioning

confidence: 99%

On-Chip Glucose Detection Based on Glucose Oxidase Encapsulated on a Platinum Modified Gold Microband Electrode

Madden¹,

Barrett²,

Laffir³

et al. 2021

Preprint

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We report a two-step electrodeposition process incorporating glucose oxidase onto a platinum- modified gold microband electrode with an o-phenylenediamine and ß-cyclodextrin mixture. The bare microband electrodes were fabricated on silicon using standard microfabrication methods i.e. lithography and etching techniques. The two-step electrode modification process was characterized using cyclic voltammetry, electrochemical impedance spectroscopy and scanning electron microscopy. The enzymatic based microband biosensor exhibited a linear response to glucose from 2.5-15 mM using both linear sweep voltammetry and chronoamperometric measurements in buffer based solutions. The resulting miniaturized glucose sensor presented a number of advantages such as ease of use, fast response time, measuring within physiologically relevant glucose concentrations in addition to sensing in small sample volumes without the need for an external counter and reference electrode. The biosensor performance was tested in 30 µl volumes of undiluted fetal bovine serum. Whilst a reduction in signal was evident within 100 % serum samples, the sensor achieved linear glucose detection with increasing glucose concentrations (2-12 mM).

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Fabrication of a novel disposable glucose biosensor using an electrochemically reduced graphene oxide–glucose oxidase biocomposite

Abstract: A disposable glucose biosensor has been fabricated on the surface of a cost-effective pencil graphite electrode (PGE) by an electrochemical method, using glucose oxidase (GOx) and reduced graphene oxide (rGO).

Cited by 25 publications

References 53 publications

Hydroxyapatite Nanoparticles Modified Graphite Electrodes for Electrochemical DNA Detection

Hydroxyapatite Nanoparticles Modified Graphite Electrodes for Electrochemical DNA Detection

On-Chip Glucose Detection Based on Glucose Oxidase Immobilized on a Platinum-Modified, Gold Microband Electrode

On-Chip Glucose Detection Based on Glucose Oxidase Encapsulated on a Platinum Modified Gold Microband Electrode

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