Fabrication of a pH responsive DOX conjugated PEGylated palladium nanoparticle mediated drug delivery system: an in vitro and in vivo evaluation

Shanthi, Krishnamurthy; Vimala, Karuppaiya; Gopi, D.; Kannan, Soundarapandian

doi:10.1039/c5ra05803a

Cited by 59 publications

(44 citation statements)

References 69 publications

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“…Herein, the DOX conjugation was proved with the decrease of fluorescence intensity and absorption peaks which was caused by the conjugation steps. In the other studies, similar cases showed up as demonstrated in Du et al 34 and Shantni et al 35 mV and -22.7±12 mV, respectively. Expectedly, the hydrodynamic particle size of AuNP-PMAA was increased after the DOX conjugation.…”

Section: Characterizationsupporting

confidence: 79%

Poly(methacrylic acid)-Coated Gold Nanoparticles: Functional Platforms for Theranostic Applications

et al. 2016

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The integration of drugs with nanomaterials have received significant interest in the efficient drug delivery systems. Conventional treatments with therapeutically active drugs may cause undesired side effects and, thus, novel strategies to perform these treatments with a combinatorial approach of therapeutic modalities are required. In this study, polymethacrylic acid coated gold nanoparticles (AuNP-PMAA), which were synthesized with reversible addition-fragmentation chain transfer (RAFT) polymerization, were combined with doxorubicin (DOX) as a model anticancer drug by creating a pH-sensitive hydrazone linkage in the presence of cysteine (Cys) and a cross-linker. Drug-AuNP conjugates were characterized via spectrofluorimetry, dynamic light scattering and zeta potential measurements as well as X-ray photoelectron spectroscopy. The particle size of AuNP-PMAA and AuNP-PMAA-Cys-DOX conjugate were calculated as found as 104 and 147 nm, respectively. Further experiments with different pH conditions (pH 5.3 and 7.4) also showed that AuNP-PMAA-Cys-DOX conjugate could release the DOX in a pH-sensitive way. Finally, cell culture applications with human cervix adenocarcinoma cell line (HeLa cells) demonstrated effective therapeutic impact of the final conjugate for both chemotherapy and radiation therapy by comparing free DOX and AuNP-PMAA independently. Moreover, cell imaging study was also an evidence that AuNP-PMAA-Cys-DOX could be a beneficial candidate as a diagnostic agent.

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Section: Characterizationsupporting

confidence: 79%

Poly(methacrylic acid)-Coated Gold Nanoparticles: Functional Platforms for Theranostic Applications

et al. 2016

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“…This notable correlation, together with the aforementioned experimental evidence indicates that the silica based nanocarriers are not only structurally fit to carry the cisplatin but also carry the phytochemical, β sitosterol to augment the anticancer activity against lung cancer, breast cancer . The similar result observed in the previous literature …”

Section: Resultsmentioning

confidence: 89%

Peptide‐Conjugated Nano‐Drug Delivery System to Improve Synergistic Molecular Chemotherapy for Colon Carcinoma

et al. 2017

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The biofunctional significance of hollow mesoporous silica nanoparticles (HMSNs) could be used as nano-reservoirs. Here in the b-sitosterol loaded into the hollow core and the cisplatin substituted with the carboxylic group of poly lactic acid (PLA)polyethylene glycol (PEG) on the pore walls of HMSNs. Subsequently, the tumour biomarker somatostatin peptide (3207-86) conjugated to Methyl polyethylene glycol 2-maleimide ethyl ether (Meo-PEG-Mal) for the selective targeting of tumour cells. The surface modified HMSNs enable the internalization of cisplatin via chlorine ion release, through the cleavage of the coordinated interaction. Further, the b sitosterol release is dependent on the discharge rate of cisplatin and the pH of the cell microenvironment. The engineered nano-drug delivery system could be used to selectively target cancer cells and deliver multimodal therapeutic schemes for the treatment of colon cancer

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“…While in apoptotic cells, an apoptotic body undergoes nuclear shrinkage and blebbing and as a result, these apoptotic cells were observed as orange colored bodies. Whereas, the loss of membrane integrity in necrotic cells showed red color fluorescence when viewed under fluorescence microscope (Figure c,d and e,f) …”

Section: Resultsmentioning

confidence: 96%

Synthesis, Biological Evaluation and Molecular Docking of Novel Curcumin Derivatives as Bcl‐2 Inhibitors Targeting Human Breast Cancer MCF‐7 Cells

2017

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An efficient one‐pot multicomponent double Michael addition strategy was developed for the synthesis of novel curcumin derivatives using 1,4‐diazabicyclo [2.2.2] octane (DABCO) as the catalyst. Selected compounds (E)‐4,4′′,5′‐trihydroxy‐6′‐(3‐(4‐hydroxy‐3‐methoxyphenyl)acryloyl)‐3,3′′‐dimethoxy‐3′,4′‐dihydro‐[1, 1′:3′, 1′′‐terphenyl]‐2′,2′(1′H)‐dicarbonitrile (4 k) and (E)‐ethyl 2′‐cyano‐4′′,5′‐dihydroxy‐6′‐(3‐(4‐hydroxy‐3‐methoxyphenyl)acryloyl)‐3′′‐methoxy‐4‐methyl‐1′,2′,3′,4′‐tetrahydro‐[1, 1′:3′, 1′′‐terphenyl]‐2′‐carboxylate (6 j) were evaluated for their in‐vitro anticancer activities against human breast cancer cells (MCF‐7) and human normal breast cells (HBL 100) and found to show effective cytotoxicity on MCF‐7 cells and less cytotoxicity on HBL 100 cells than simple curcumin. In addition, molecular docking studies helped to rationalize anti‐apoptotic Bcl‐2 binding of all the synthesized compounds and revealed that the docking of compounds 4 k and 6 j with Bcl‐2 was more potent compared to curcumin.

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Fabrication of a pH responsive DOX conjugated PEGylated palladium nanoparticle mediated drug delivery system: an in vitro and in vivo evaluation

Abstract: Schematic illustration of the possible mechanism of pH based drug delivery system of DOX conjugated PEGylated PdNPs induced apoptosis in HeLa cells.

Cited by 59 publications

References 69 publications

Poly(methacrylic acid)-Coated Gold Nanoparticles: Functional Platforms for Theranostic Applications

Poly(methacrylic acid)-Coated Gold Nanoparticles: Functional Platforms for Theranostic Applications

Peptide‐Conjugated Nano‐Drug Delivery System to Improve Synergistic Molecular Chemotherapy for Colon Carcinoma

Synthesis, Biological Evaluation and Molecular Docking of Novel Curcumin Derivatives as Bcl‐2 Inhibitors Targeting Human Breast Cancer MCF‐7 Cells

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