Fabrication of Amyloid Curli Fibers–Alginate Nanocomposite Hydrogels with Enhanced Stiffness

Axpe, Eneko; Duraj-Thatte, Anna; Chang, Yin; Kaimaki, Domna-Maria; Sanchez‐Sanchez, Ana; Caliskan, Huseyin Burak; Courchesne, Noémie‐Manuelle Dorval; Joshi, Neel

doi:10.1021/acsbiomaterials.8b00364

Cited by 32 publications

(47 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Engineering of 'truly' living materials where the living component actively facilitates material fabrication and organization is much more challenging. True ELMs have been created by engineering Escherichia coli to produce an extracellular matrix from curli fibers [8][9][10][11][12][13][14][15][16][17][18][19][20][21][22][23][24][25] . Other types of extracellular matrices for ELM fabrication were created from secreted bacterial cellulose to embed microbial cells 26,27 or from elastin-like polypeptides to attach Caulobacter cells via their protein S-layers 28 .…”

mentioning

confidence: 99%

Engineering Bacillus subtilis for the formation of a durable living biocomposite material

et al. 2021

View full text Add to dashboard Cite

Engineered living materials (ELMs) are a fast-growing area of research that combine approaches in synthetic biology and material science. Here, we engineer B. subtilis to become a living component of a silica material composed of self-assembling protein scaffolds for functionalization and cross-linking of cells. B. subtilis is engineered to display SpyTags on polar flagella for cell attachment to SpyCatcher modified secreted scaffolds. We engineer endospore limited B. subtilis cells to become a structural component of the material with spores for long-term storage of genetic programming. Silica biomineralization peptides are screened and scaffolds designed for silica polymerization to fabricate biocomposite materials with enhanced mechanical properties. We show that the resulting ELM can be regenerated from a piece of cell containing silica material and that new functions can be incorporated by co-cultivation of engineered B. subtilis strains. We believe that this work will serve as a framework for the future design of resilient ELMs.

show abstract

mentioning

confidence: 99%

Engineering Bacillus subtilis for the formation of a durable living biocomposite material

et al. 2021

View full text Add to dashboard Cite

show abstract

“…Applied to produce hydroxyapatite (HAP)-containing curli-like amyloid fibers [ 131 ] Amyloid curli fibers–alginate nanocomposite Amyloid supplementation to alginate hydrogels for enhanced stiffness. [ 132 ] Electron transfer curli nanofibers Engineered E. coli system for the synthesis of conductive protein networks. [ 133 ] …”

Section: Engineering Cells To Synthesize (Precursors Of) Non-living Materialsmentioning

confidence: 99%

Synthetic biology as driver for the biologization of materials sciences

Burgos-Morales

Gueye

Lacombe

et al. 2021

Materials Today Bio

View full text Add to dashboard Cite

Materials in nature have fascinating properties that serve as a continuous source of inspiration for materials scientists. Accordingly, bio-mimetic and bio-inspired approaches have yielded remarkable structural and functional materials for a plethora of applications. Despite these advances, many properties of natural materials remain challenging or yet impossible to incorporate into synthetic materials. Natural materials are produced by living cells, which sense and process environmental cues and conditions by means of signaling and genetic programs, thereby controlling the biosynthesis, remodeling, functionalization, or degradation of the natural material. In this context, synthetic biology offers unique opportunities in materials sciences by providing direct access to the rational engineering of how a cell senses and processes environmental information and translates them into the properties and functions of materials. Here, we identify and review two main directions by which synthetic biology can be harnessed to provide new impulses for the biologization of the materials sciences: first, the engineering of cells to produce precursors for the subsequent synthesis of materials. This includes materials that are otherwise produced from petrochemical resources, but also materials where the bio-produced substances contribute unique properties and functions not existing in traditional materials. Second, engineered living materials that are formed or assembled by cells or in which cells contribute specific functions while remaining an integral part of the living composite material. We finally provide a perspective of future scientific directions of this promising area of research and discuss science policy that would be required to support research and development in this field.

show abstract

“…As such it is an attractive platform for a variety of applications, such as bioremediation to sequester toxins from the environment 53 , or for in vivo therapeutic use 54 . The mechanical properties of curli fibres have not been extensively studied in isolation; however, they can provide stiffness to natural biofilms 55 , and when added to alginate gels 56 .…”

Section: Protein Materialsmentioning

confidence: 99%