Fabrication of an efficient and sensitive colorimetric biosensor based on Uricase/ Th-MOF for uric acid sensing in biological samples

Badoei-dalfard, Arastoo; Sohrabi, Nasrin; Karami, Zahra; Sargazi, Ghasem

doi:10.1016/j.bios.2019.111420

Cited by 122 publications

(34 citation statements)

References 56 publications

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“…Recently, coupling with the concept of single-atom catalysis, the rational regulation of the coordination environment of active sites at the atomic scale can endow SAzymes with high activity and selectivity. [60,139] On the other hand, taking advantage of the ligand engineering of MOFs, the surface microenvironment of the encapsulated catalysts can be tuned. [140] Based on this phenomenon, reasonable combination of nanozymes and MOFs is a promising method to boost the activity of nanozymes.…”

Section: Discussionmentioning

confidence: 99%

“…As stated above, the intrinsic enzyme‐like activity of MOFs can be used to construct enzymes–nanozymes cascade systems as well. [ 60 ] Qu and co‐workers employed an ultrathin 2D MOF (2D Cu‐TCPP (Fe)) nanosheet as a POD mimic to immobilize GOx through physical adsorption for self‐induced cascade wound healing in vivo. [ 57 ] Furthermore, to improve the stability of immobilized enzymes, our group used the Fe‐MIL‐88B‐NH 2 (Fe‐MOF) with inherent POD‐like activity to directly immobilize the GOx by covalent binding for the establishment of an integrated enzyme system (Figure 3d).…”

Section: Construction Of Mof‐involving Biomimetic Cascade Systemsmentioning

confidence: 99%

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Metal–Organic Frameworks Enhance Biomimetic Cascade Catalysis for Biosensing

Jiao

et al. 2021

Advanced Materials

147

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Multiple enzymes‐induced biological cascade catalysis guides efficient and selective substrate transformations in vivo. The biomimetic cascade systems, as ingenious strategies for signal transduction and amplification, have a wide range of applications in biosensing. However, the fragile nature of enzymes greatly limits their wide applications. In this regard, metal–organic frameworks (MOFs) with porous structures, unique nano/microenvironments, and good biocompatibility have been skillfully used as carriers to immobilize enzymes for shielding them against hash surroundings and improving the catalytic efficiency. For another, nanomaterials with enzyme‐like properties and brilliant stabilities (nanozymes), have been widely applied to ameliorate the low stability of the enzymes. Inheriting the abovementioned merits of MOFs, the performances of MOFs‐immboilized nanozymes could be significantly enhanced. Furthermore, in addition to carriers, some MOFs can also serve as nanozymes, expanding their applications in cascade systems. Herein, recent advances in the fabrication of efficient MOFs‐involving enzymes/nanozymes cascade systems and biosensing applications are highlighted. Integrating diversified signal output modes, including colorimetry, electrochemistry, fluorescence, chemiluminescence, and surface‐enhanced Raman scattering, sensitive detection of various targets (including biological molecules, environmental pollutants, enzyme activities, and so on) are realized. Finally, challenges and opportunities about further constructions and applications of MOFs‐involving cascade reaction systems are briefly put forward.

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Section: Discussionmentioning

confidence: 99%

Section: Construction Of Mof‐involving Biomimetic Cascade Systemsmentioning

confidence: 99%

Metal–Organic Frameworks Enhance Biomimetic Cascade Catalysis for Biosensing

Jiao

et al. 2021

Advanced Materials

147

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“…Various other nanobased systems have been developed, characterized, and explored [ 133 , 134 , 135 ]. Because of the colloidal stability and electro-optical properties, AuNPs have gained attention in the past for diagnostic purposes.…”

Section: Detection Of Snpsmentioning

confidence: 99%

Application of Nanotechnology for Sensitive Detection of Low-Abundance Single-Nucleotide Variations in Genomic DNA: A Review

et al. 2021

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Single-nucleotide polymorphisms (SNPs) are the simplest and most common type of DNA variations in the human genome. This class of attractive genetic markers, along with point mutations, have been associated with the risk of developing a wide range of diseases, including cancer, cardiovascular diseases, autoimmune diseases, and neurodegenerative diseases. Several existing methods to detect SNPs and mutations in body fluids have faced limitations. Therefore, there is a need to focus on developing noninvasive future polymerase chain reaction (PCR)–free tools to detect low-abundant SNPs in such specimens. The detection of small concentrations of SNPs in the presence of a large background of wild-type genes is the biggest hurdle. Hence, the screening and detection of SNPs need efficient and straightforward strategies. Suitable amplification methods are being explored to avoid high-throughput settings and laborious efforts. Therefore, currently, DNA sensing methods are being explored for the ultrasensitive detection of SNPs based on the concept of nanotechnology. Owing to their small size and improved surface area, nanomaterials hold the extensive capacity to be used as biosensors in the genotyping and highly sensitive recognition of single-base mismatch in the presence of incomparable wild-type DNA fragments. Different nanomaterials have been combined with imaging and sensing techniques and amplification methods to facilitate the less time-consuming and easy detection of SNPs in different diseases. This review aims to highlight some of the most recent findings on the aspects of nanotechnology-based SNP sensing methods used for the specific and ultrasensitive detection of low-concentration SNPs and rare mutations.

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“…By incorporating with urease, a sensitive colorimetric biosensor was developed for the detection of uric acid in biological samples. [ 43 ] The peroxidase‐like activity of Cu‐MOFs has also be used to develop an electrochemical signal amplifying immune probe by combining with GOx and CA15‐3 antibody for the ultrasensitive detection of CA15‐3. [ 44 ] By utilizing the oxidase mimic property and fluorescence property of amino‐functionalized copper (II)‐MOFs (NH 2 ‐Cu‐MOFs), a ratiometric multicolor fluorescence biosensor was developed for the visual detection of alkaline phosphatase activity.…”

Section: Mofs As Enzyme‐mimic Elementsmentioning

confidence: 99%

Applications of Functional Metal‐Organic Frameworks in Biosensors

Chen

Zhu

et al. 2020

Biotechnology Journal

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In recent decades, fast advancements in the fields of metal‐organic frameworks (MOFs) are providing unprecedented opportunities for the development of novel functional MOFs for various biosensing applications. Exciting progress is achieved due to the combination of MOFs with various functional components, which introduces novel structures and new features to the MOFs‐based biosensing applications, such as higher stability, higher sensitivity, higher flexibility, and higher specificity. This review aims to be a comprehensive summary of the most recent advances in the development of functional MOFs for various biosensing applications, placing special attention on important contributions in recent 3 years. In this review, the most recent developments in design and synthesis of functional MOFs for biosensing applications are summarized. MOFs‐based biosensing applications are outlined according to the central roles of MOFs in biosensors, which include carriers of sensitive elements, enzyme‐mimic elements, electrochemical signaling, optical signaling, and gas sensing. Finally, the current challenges and future development trends of functional MOFs for biosensing applications are proposed and discussed.

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Fabrication of an efficient and sensitive colorimetric biosensor based on Uricase/ Th-MOF for uric acid sensing in biological samples

Cited by 122 publications

References 56 publications

Metal–Organic Frameworks Enhance Biomimetic Cascade Catalysis for Biosensing

Metal–Organic Frameworks Enhance Biomimetic Cascade Catalysis for Biosensing

Application of Nanotechnology for Sensitive Detection of Low-Abundance Single-Nucleotide Variations in Genomic DNA: A Review

Applications of Functional Metal‐Organic Frameworks in Biosensors

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