Fabrication of artificial fluorescent protein probe for HSA recognition and relay sensing Ag + by functional microenvironment-sensitive fluorescent dye

Du, Jianjun; Tao, Zhu; Gu, Quanyong; Cao, Wenbing; Fan, Jiangli; Peng, Xiaojun

doi:10.1016/j.snb.2018.02.154

Cited by 24 publications

(6 citation statements)

References 29 publications

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“…[ 14–16,41,45 ] It has been observed that NAP‐HS can efficiently detect and quantify HSA in pathological samples having relatively higher HSA concentration in mg mL −1 . However, there are other sensitive HSA selective probes known in literature whose LOD values are lower (as low as 1.95–91.9 nM) [ 58–62 ] than NAP‐HS. But, most of these reported probes do not quantify serum albumin concentration directly from the pathological samples.…”

Section: Resultsmentioning

confidence: 99%

Bimodal Turn‐On Fluorescent Probe for Photophysical and Electrochemical Detection of Human Serum Albumin in Clinical Samples

Saha

Moitra

Bhattacharjee

et al. 2022

Adv Materials Inter

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A simple, easy to synthesize, cost‐effective fluorescent turn‐on sensor is developed for the selective detection of human serum albumin (HSA) in both biological and environmental samples. The biosensor is unique with qualities of rapidly quantifying HSA levels both at lower and higher concentrations in divergent bio‐fluids with high sensitivity. This probe has also been successfully used for the quantitative determination of serum albumin levels in a large number of patients’ samples having different clinical manifestations. Electrochemical detection of HSA concentrations can also be achieved with this biosensor using a potentiostat. Thus, this probe offers a unique potential of diagnosing HSA levels directly from patient samples using its bimodal (i.e., photophysical and electrochemical) properties, which are hitherto unknown to date.

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Section: Resultsmentioning

confidence: 99%

Bimodal Turn‐On Fluorescent Probe for Photophysical and Electrochemical Detection of Human Serum Albumin in Clinical Samples

Saha

Moitra

Bhattacharjee

et al. 2022

Adv Materials Inter

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“…In this work, a salicylaldehyde derivative, 4,4 ′ -(hydrazine-1,2diylidene bis(methanylylidene)) bis(3-hydroxybenzoic acid) (HDBB), was synthesized by a one-step method. Various approaches were used to characterize the structure of HDBB, including FTIR, 1 HNMR, 13 CNMR, and HRMS. The obtained HDBB was an AIE molecule with ESIPT characteristics, and it was used as a uorescence probe for the detection of HSA with a good linear relationship between 0.01 and 0.2 mg mL −1 and a detection limit of 1.104 mg mL −1 .…”

Section: Discussionmentioning

confidence: 99%

“…Fluorescence (FL) spectra were recorded on a Hitachi F-4600 uorescence spectrophotometer (Tokyo, Japan), with an excitation wavelength of 415 nm (slit: EX = 5 nm, EM = 20 nm). 1 H NMR and 13 C NMR spectra were recorded using DMSO-d 6 as the solvent on an AVANCE NEO 400 MHz nuclear magnetic resonance spectrometer. Highresolution mass spectrometry (HRMS) was performed on a liquid chromatography-ion trap-time of ight mass spectrometer (Shimadzu, Japan).…”

Section: Chemicals and Reagentsmentioning

confidence: 99%

“…In this work, we used a one-step method to synthesize HDBB from 4-formyl-3-hydroxybenzoic acid and hydrazine hydrate in methanol. To conrm the successful preparation of HDBB, we performed spectroscopic tests such as FTIR, 1 H NMR, 13 C NMR, and HRMS on HDBB (Fig. 1).…”

Section: Structural Characterization Of Hdbbmentioning

confidence: 99%

“…At present, there are numerous methods for detecting HSA, such as liquid chromatography-mass spectrometry, colorimetry, electrochemistry, uorescence, etc. [10][11][12][13] The uorescence method has features such as simple operation, fast reaction, high sensitivity, and stability, attracting much attention. For example, Xu et al developed a uorescent probe based on BODIPY for detecting HSA in bodily uids.…”

Section: Introductionmentioning

confidence: 99%

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An HDBB-based fluorescent probe for the sensitive detection of human serum albumin

Wang,

et al. 2024

Anal. Methods

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An Intelligent AIEgen with Nonmonotonic Multiresponses to Multistimuli

Xiao

et al. 2020

Advanced Science

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Intelligent stimulus-response (S/R) systems are the basis of natural process and machine control, which are intensively explored in biomimetic design and analytical/biological applications. However, nonmonotonic multi-S/R systems are still rarely studied so far. In this work, a rational design strategy is proposed to achieve such a unique S/R system by integrating opposite luminescence behaviors in one molecule. When solvent polarity increases, many heterocyclic or carbonyl-containing compounds often become more emissive due to the suppression of the proximity effect, whereas molecules with donor-acceptor (D-A) structures tend to be less emissive because of the twisted intramolecular charge transfer. Meanwhile, protonation on D/A moieties will weaken/strengthen the D-A interaction to result in blue/redshifted emissions. By combining a protonatable heterocyclic acceptor and a protonatable donor together in one molecule, nonmonotonic brightness responses to polarity stimuli and nonmonotonic color responses to pH stimuli can be achieved. The design strategy is successfully verified by a simple molecule named 4-(dimethylamino)styryl)quinoxalin-2(1H)-one (ASQ). ASQ exhibits nonmonotonic responses to polarity and pH stimuli, and aggregation-induced emission (AIE) with a nonmonotonic AIE curve. Meanwhile, ASQ can be adjusted to emit white light in an acidic environment, and it shows multivalent functionalities including albumin protein sensing, ratiometric pH sensing, and amine gas sensing.

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Fabrication of artificial fluorescent protein probe for HSA recognition and relay sensing Ag + by functional microenvironment-sensitive fluorescent dye

Cited by 24 publications

References 29 publications

Bimodal Turn‐On Fluorescent Probe for Photophysical and Electrochemical Detection of Human Serum Albumin in Clinical Samples

Bimodal Turn‐On Fluorescent Probe for Photophysical and Electrochemical Detection of Human Serum Albumin in Clinical Samples

An HDBB-based fluorescent probe for the sensitive detection of human serum albumin

An Intelligent AIEgen with Nonmonotonic Multiresponses to Multistimuli

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