Fabrication of bioactive conduits containing the fibroblast growth factor 1 and neural stem cells for peripheral nerve regeneration across a 15 mm critical gap

Ni, Hsiao-Chiang; Tseng, Ting-Chen; Chen, Jeng-Rung; Hsu, Shan‐hui; Chiu, Ing‐Ming

doi:10.1088/1758-5082/5/3/035010

Cited by 65 publications

(38 citation statements)

References 40 publications

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“…However, the only source of SCs is to sacrifice a healthy nerve (analogous to an autograft) and SCs do not seem to proliferate well under cell culture environments (28,29). In seeking alternatives, researchers are investigating other cell types for nerve regeneration strategies such as olfactory ensheathing cells (30,31,32,33), neural stem cells (34,35,36,37) and MSCs derived from various sources (bone marrow (38,39), adipose derived (38,40), These tMSCs were characterized and compared with undifferentiated MSCs (uMSCs) by immunostaining using glial markers such as α-S100β, α-p75 NTR and α-GFAP (8,13,45). Ladak et.al.…”

Section: Transdifferentiation Of Mscsmentioning

confidence: 99%

Oriented growth and transdifferentiation of mesenchymal stem cells towards a Schwann cell fate on micropatterned substrates

Sharma

Zbarska

Petersen

et al. 2016

Journal of Bioscience and Bioengineering

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While Schwann cells (SCs) have a significant role in peripheral nerve regeneration, their use in treatments has been limited because of lack of a readily available source. To address this issue, this study focused on the effect of guidance cues by employing micropatterned polymeric films to influence the alignment, morphology and transdifferentiation of bone marrow-derived rat mesenchymal stem cells (MSCs) towards a Schwann cell-like fate. Two different types of polymers, biocompatible polystyrene (PS) and biodegradable poly(lactic acid) (PLA) were used to fabricate patterned films. Percentages of transdifferentiated MSCs (tMSCs) immunolabeled with SC markers (α-S100β and α-p75(NTR)) were found to be similar on patterned versus smooth PS and PLA substrates. However, patterning had a significant effect on the alignment and elongation of the tMSCs. More than 80% of the tMSCs were oriented in the direction of microgrooves (0°-20°), while cells on the smooth substrates were randomly oriented. The aspect ratio [AR, ratio of length (in direction of microgrooves) and breadth (in direction perpendicular to microgrooves)] of the tMSCs on patterned substrates had a value of approximately five, as compared to cells on smooth substrates where the AR was one. Understanding responses to these cues in vitro helps us in understanding the behavior and interaction of the cells with the 3D environment of the scaffolds, facilitating the application of these concepts to designing effective nerve guidance conduits for peripheral nerve regeneration.

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Section: Transdifferentiation Of Mscsmentioning

confidence: 99%

Oriented growth and transdifferentiation of mesenchymal stem cells towards a Schwann cell fate on micropatterned substrates

Sharma

Zbarska

Petersen

et al. 2016

Journal of Bioscience and Bioengineering

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“…There are several clinical strategies to restore nerve function, including artificial chambers [11], neurotrophic factors [12], stem cells [13], and nerve grafting [14]. Despite the application of modern and advanced techniques for peripheral nerve injuries, functional outcomes are often unpredictable and disappointing.…”

Section: Discussionmentioning

confidence: 99%

“…And, at 0 min and 14 days after sciatic nerve injury, there was no any recovery of motor function. At 42 days after SNI, the SFI in SNI + COS group was better than in the SNI group and SNI + vehicle group (*p<0.05, **p<0.01, n= 8) 13.3×(EIT − NIT) / NIT−8.8. SFI is the sciatic nerve function index, E indicates the experimental (injured) foot, N indicates the normal foot, PL is the length from the tip of the toe to the heel, TS is the span between the first and fifth toes, and IT is the span between the second and fourth toes.…”

Section: Footprint Experimentsmentioning

confidence: 94%

Chitooligosaccharide Inhibits Scar Formation and Enhances Functional Recovery in a Mouse Model of Sciatic Nerve Injury

Hou

Zhang

et al. 2015

Mol Neurobiol

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Chitooligosaccharide (COS) has been shown to induce fibroblast apoptosis, indicating that it could be used as a material to inhibit scar formation. In the present study, we used a mouse model of sciatic nerve injury (SNI) to determine the role of COS in scar inhibition and functional recovery. The animals were divided into three groups: SNI, SNI + vehicle, and SNI + COS group. We performed a series of functional and histological examinations at ctrl, 0 min, 14 days, and 42 days, including behavioral recovery, percentage of regenerating axons, degree of scar formation, vascular changes, type I and type III collagen ratio, and percentage of demyelinated axons. The SNI + COS group exhibited better recovery of sensory and motor function and less scar formation. Two-photon microscopy showed that the percentage of regenerating axons was highest in the SNI + COS group at 14 and 42 days. Our results suggested that COS can inhibit scar formation and enhance functional recovery by inducing fibroblast death, altering the proportion of different vascular diameters, changing the ratio of type I/type III collagen, and reducing the percentage of demyelinated axons. COS might be a useful drug in the treatment of SNI to reduce scar formation, but additional research is required to clarify the relevant molecular pathways.

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“…Current literature evaluating CatWalk only employ a single injury group and a sham group, so to emulate current research practices, we employed three experimental groups, each receiving nerve resection injury followed by different interventions leading to different levels of recovery: one with an autologous nerve graft (clinical gold standard) to serve as positive control, one with an empty nerve conduit to serve as negative control, and one with human neural crest stem cell (NCSC) implantation to serve as the study target (Bozkurt et al, 2008), which leads to improvements in recovery that is superior to empty nerve conduits but not as effective as autologous nerve grafts (Georgiou et al, 2015; Ni et al, 2013; Wang et al, 2015). …”

Section: Introductionmentioning

confidence: 99%

Establishing a reliable gait evaluation method for rodent studies

Chen

Zhang

et al. 2017

Journal of Neuroscience Methods

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BACKGROUND CatWalk is one of the most popular tools for evaluating gait recovery in preclinical research, however, there is currently no consensus on which of the many gait parameters captured by CatWalk can reliably model recovery. There are conflicting interpretations of results, along with many common but seldom reported problems such as heel walking and poor compliance. NEW METHOD We developed a systematic manual classification method that overcomes common problems such as heel walking and poor compliance. By correcting automation errors and removing inconsistent gait cycles, we isolated stretches of recordings that are more reliable for analysis. Recovery outcome was also assessed by quantitative histomorphometric analysis of myelinated axons. RESULTS While 40–60% of runs were erroneously classified without manual intervention, we corrected all errors with our new method, and showed that Stand Time, Duty Cycle, and Swing Speed are able to track significant differences over time and between experimental groups (all p<0.05). The usability of print area and intensity parameters requires further validation beyond the capabilities of CatWalk. COMPARISON WITH EXISTING METHOD(S) There is currently no strategy that addresses problems such as heel walking and poor compliance, and therefore no standard set of parameters that researchers can rely on to report their findings. CONCLUSION Manual classification is a crucial step to generate reliable CatWalk data, and Stand Time, Duty Cycle, and Swing Speed are suitable parameters for evaluating gait recovery. Static parameters such as print area and intensity should be used with extreme caution.

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Fabrication of bioactive conduits containing the fibroblast growth factor 1 and neural stem cells for peripheral nerve regeneration across a 15 mm critical gap

Cited by 65 publications

References 40 publications

Oriented growth and transdifferentiation of mesenchymal stem cells towards a Schwann cell fate on micropatterned substrates

Oriented growth and transdifferentiation of mesenchymal stem cells towards a Schwann cell fate on micropatterned substrates

Chitooligosaccharide Inhibits Scar Formation and Enhances Functional Recovery in a Mouse Model of Sciatic Nerve Injury

Establishing a reliable gait evaluation method for rodent studies

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