Fabrication of biocompatible lab‐on‐chip devices for biomedical applications by means of a 3D‐printing process

Abstract

A new microfluidic assembly method for semiconductor-based biosensors using 3D-printing technologies was proposed for a rapid and cost-efficient design of new sensor systems. The microfluidic unit is designed and printed by a 3D-printer in just a few hours and assembled on a light-addressable potentiometric sensor (LAPS) chip using a photo resin. The cell growth curves obtained from culturing cells within microfluidics-based LAPS systems were compared with cell growth curves in cell culture flasks to examine biocompatibility of the 3D-printed chips. Furthermore, an optimal cell culturing within microfluidics-based LAPS chips was achieved by adjusting the fetal calf serum concentrations of the cell culture medium, an important factor for the cell proliferation.

Feasibility and Biocompatibility of 3D‐Printed Photopolymerized and Laser Sintered Polymers for Neuronal, Myogenic, and Hepatic Cell Types

“…3D‐printed biocompatible polymers hold significant importance for tissue and biochemical engineering applications, with the methods required to construct the models that examine highly complex cellular physiological processes currently being revolutionized by AM . Pertinent to this are tissue engineered in vitro models, designed to replicate the in vivo cellular and molecular mechanisms that regulate musculoskeletal and neuromuscular disorders .…”

“…[19] 3D-printed biocompatible polymers hold significant importance for tissue and biochemical engineering applications, with the methods required to construct the models that examine highly complex cellular physiological processes currently being revolutionized by AM. [6,[20][21][22][23][24][25][26][27] Pertinent to this are tissue engineered in vitro models, [28][29][30][31] designed to replicate the in vivo cellular and molecular mechanisms that regulate musculoskeletal [32] and neuromuscular disorders. [33] As such, defining the compatibility of mammalian cells frequently used within these models, C 2 C 12 murine skeletal myoblasts [34] and SH-SY5Y neuroblastoma [35] human derived cell lines are of particular relevance.…”

Moving from millifluidic to truly microfluidic sub-100-μm cross-section 3D printed devices

“…Mandon and coworkers [20] looked at printing hydrogels with embedded enzymes to set up sequences of reactions; importantly, they demonstrated the use of multiple materials with SLA printing. Takenaga et al [21] monitored H + concentrations by photocurrent detected in cultured cells by sealing their device and sandwiching it between a silicon chip and glass cover slip. However, having at least one dimension >1 mm limits the potential applications of these fluidic devices.…”

Magnetic Microreactors with Immobilized Enzymes—From Assemblage to Contemporary Applications

“…It is also noteworthy that 3D printing technology bares the potential to generate sterilizable products, thus expanding the scope of feasible biological applications. Three-dimensional printed microreactors have been reported to consist of biocompatible and photo-curable resins, while heat and organic solvent resistant resins are also currently incorporated in this upcoming technology [12,13].…”