Fabrication of Biomimetically Patterned Surfaces and Their Application to Probing Plant–Bacteria Interactions

Zhang, Boce; Luo, Yaguang; Pearlstein, Arne J.; Aplin, Jesse J.; Liu, Yi; Bauchan, Gary R.; Payne, Gregory F.; Wang, Qin; Nou, Xiangwu; Millner, Patricia D.

doi:10.1021/am502384q

Cited by 52 publications

(64 citation statements)

References 37 publications

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“…The Hirox Digital Microscope System supported magnifications of up to 7000×, and the whole 3‐D image was formed by the reconstruction of the images taken at number of focal planes under the focused area of the sample. The inbuilt surface analyzing software formed a graphical picture of the analyzed region …”

Section: Methodsmentioning

confidence: 99%

Novel green surface modification of metallocene polyethylene by steam to enhance its hemocompatible properties

John

Jaganathan

Supriyanto

et al. 2016

J of Applied Polymer Sci

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Steam treatment is a green surface modification technique that was used to improve the surface characteristics and hemocompatibility of metallocene polyethylene (mPE). In this study, a sharp decrease in the mean contact angle of steam-exposed mPE compared to that of untreated mPE showed enhanced hydrophilicity. The increased surface roughness was demonstrated by atomic force microscopy, scanning electron microscopy, and Hirox three-dimensional microscopy. The average roughness of the control mPE (2.757 nm) was enhanced to 8.753 nm by steam treatment. Fourier transform infrared spectroscopy analysis illustrated no chemical changes, but the changes in the absorbance intensity ensured morphological changes. Blood compatibility studies were assessed by coagulation assays, hemolysis, and platelet adhesion tests. The mean number of platelets adhered to the steam-treated sample (11) was half of the number of platelets adhered to the untreated mPE surface (22). The clotting time on the steam exposed surface was delayed, hemolysis and platelet adhesion were significantly reduced. The green surface modification of mPE with steam enhanced its surface properties and hemocompatibility. The improved blood compatibility of mPE may help in the efficient designing of hemocompatible biomaterials such as cardiovascular implants.

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Section: Methodsmentioning

confidence: 99%

Novel green surface modification of metallocene polyethylene by steam to enhance its hemocompatible properties

John

Jaganathan

Supriyanto

et al. 2016

J of Applied Polymer Sci

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“…topography, roughness, and hydrophobicity (Boyd, Verran, Jones, & Bhakoo, 2002;Erickson, 2012a;Fernandes et al, 2014;Gomes et al, 2009;Hsu, Fang, Borca-Tasciuc, Worobo, & Moraru, 2013;Liu et al, 2004;Wang, Feng, Liang, Luo, & Malyarchuk, 2009;Wang, Zhou, & Feng, 2012;Yadav, Karamanoli, & Vokou, 2005). However, the role that each surface property plays in bacterial retention remains poorly understood (Erickson, 2012b;Wang et al, 2012;Wang, Feng, Luo, & Zhang, 2007;Zhang et al, 2014). Furthermore, produce surface properties have also been found to influence the cleaning efficacy of produce (Wang et al, 2007;.…”

Section: Introductionmentioning

confidence: 99%

“…In this study, a simple method using polydimethylsiloxane (PDMS) was developed to fabricate tomato, spinach, and lettuce surfaces, which represents a wide range of surface topographical features. PDMS, a nontoxic silicon-based polymer that is malleable, optically transparent, and chemically resistive, has many applications in the casting and fabrication of designed surfaces (Hou, Gu, Smith, & Ren, 2011;Leclerc, Sakai, & Fujii, 2003;Liu & Li, 2012;Mata, Fleischman, & Roy, 2005;Sun et al, 2005;Warning & Datta, 2013;Zhang et al, 2014). However, the application of PDMS toward the fabrication of fresh produce surfaces for microbial attachment studies is limited (Warning & Datta, 2013;Zhang et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

“…PDMS, a nontoxic silicon-based polymer that is malleable, optically transparent, and chemically resistive, has many applications in the casting and fabrication of designed surfaces (Hou, Gu, Smith, & Ren, 2011;Leclerc, Sakai, & Fujii, 2003;Liu & Li, 2012;Mata, Fleischman, & Roy, 2005;Sun et al, 2005;Warning & Datta, 2013;Zhang et al, 2014). However, the application of PDMS toward the fabrication of fresh produce surfaces for microbial attachment studies is limited (Warning & Datta, 2013;Zhang et al, 2014). While it is more relevant and realistic to use real fruit or vegetable surfaces for studying bacterial attachment and such, the extremely heterogeneous nature of these surfaces hinders our ability to obtain a fundamental understanding of important phenomena and the key processes/factors involved.…”

Section: Introductionmentioning

confidence: 99%

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Polydimethylsiloxane Replicas Efficacy for Simulating Fresh Produce Surfaces and Application in Mechanistic Study of Colloid Retention

Sun

Lazouskaya

Jin

2019

Journal of Food Science

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Foodborne illnesses associated with fresh produce have attracted increasing attention in the food industry, scientific and public health communities. Studies have shown that surface properties of fresh produce can affect bacterial attachment and colonization, yet the mechanisms involved remain poorly understood. In our previous work, using colloids as bacterial surrogates, we demonstrated that colloid retention on fresh produce was controlled by water retention/distribution on produce surfaces, which were in turn governed by produce surface properties. However, high variabilities among the natural samples and multiple factors that were simultaneously involved made it difficult for interpreting the results and in pinpointing the mechanisms responsible for the observed colloid retention behavior. To better evaluate the mechanisms, polydimethylsiloxane (PDMS) replicas of tomato, lettuce, and spinach were fabricated and compared with fresh produce surfaces in this study. The PDMS replicas thoroughly preserved the surface topographical features of their natural counterparts while having identical chemical properties (for example, hydrophobicity), thus, allowing for the separation of surface topography/roughness and hydrophobicity effects. We found that residual water retention/distribution and colloid retention on the PDMS replicas were consistent with the results observed on the corresponding fresh produce surfaces, but had smaller deviations from the respective means when compared to the natural surfaces. The use of PDMS replicas improved experimental reproducibility, and enabled differentiation on the effects of surface hydrophobicity and surface roughness on colloid retention, thus, allowed more rigorous elucidation of the underlying mechanisms. Therefore, PDMS replicas could be used as surrogates of fresh produce for mechanistic studies of surface‐bacteria interactions. Practical Application This work demonstrates the feasibility of using polydimethylsiloxane (PDMS) to simulate fresh produce surfaces for studying interactions between produce surfaces and colloids, including bacteria. Although it is more realistic to use fruit or vegetable surfaces, the difficulties of working with natural surfaces that are heterogeneous and variable hinder systematic and mechanistic studies. The use of PDMS replicas can eliminate these difficulties and improve experimental reproducibility. This study demonstrated that PDMS replicas could adequately represent the topographical features of natural produce surfaces; the results on colloid retention provided insight into fresh produce contamination and the development of effective decontamination strategies.

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“…21,22 Thus, protocols utilizing double-casting approaches, have been reported to develop leaf replicas in agar, epoxy, polystyrene, and polydimethylsiloxane (PDMS). [22][23][24][25][26] Fabricating replica surfaces inspired by nature, such as leaves have primarily been focused on replicating their superhydrophobic, superoleophobic, and superhydrophilic properties. [2][3][4][5] Such surfaces have applications in anti-reflection, anti-fouling, and anti-fogging coatings.…”

Section: Introductionmentioning

confidence: 99%

Replicating Arabidopsis Model Leaf Surfaces for Phyllosphere Microbiology

Soffe

Bernach

Remus-Emsermann

et al. 2019

Preprint

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words)Artificial surfaces are commonly used in place of leaves in phyllosphere microbiology to study microbial behaviour on plant leaf surfaces. Studies looking into individual environmental factors influencing microorganisms are routinely carried out using artificial surfaces.Commonly used artificial surfaces include nutrient agar, isolated leaf cuticles, and reconstituted leaf waxes. However, interest is growing in using microstructured surfaces mimicking the complex topography of leaf surfaces for phyllosphere microbiology. As such replica leaf surfaces, produced by microfabrication, are appearing in literature. Replica leaf surfaces have been produced in agar, epoxy, polystyrene, and polydimethylsiloxane (PDMS). However, these protocols are not suitable for replicating fragile leaves such as of the model plant Arabidopsis thaliana. This is of importance as A. thaliana is a model system for molecular plant genetics, molecular plant biology, and microbial ecology. Here we present a versatile replication protocol for replicating fragile leaf surfaces into PDMS. We display the capacity of our replication process using optical microscopy, atomic force microscopy (AFM), and contact angle measurements to compare living and PDMS replica A. thaliana leaf surfaces. To highlight the use of our replica leaf surfaces for phyllosphere microbiology, we visualised bacteria on the replica leaf surfaces in comparison to living leaf surfaces.

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Fabrication of Biomimetically Patterned Surfaces and Their Application to Probing Plant–Bacteria Interactions

Cited by 52 publications

References 37 publications

Novel green surface modification of metallocene polyethylene by steam to enhance its hemocompatible properties

Novel green surface modification of metallocene polyethylene by steam to enhance its hemocompatible properties

Polydimethylsiloxane Replicas Efficacy for Simulating Fresh Produce Surfaces and Application in Mechanistic Study of Colloid Retention

Replicating Arabidopsis Model Leaf Surfaces for Phyllosphere Microbiology

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