Fabrication of biphasic cartilage-bone integrated scaffolds based on tissue-specific photo-crosslinkable acellular matrix hydrogels

Hua, Yujie; Huo, Yingying; Bai, Baoshuai; Hao, Junxiang; Hu, Guanhuai; Ci, Zheng; Wu, Xiaodi; Yu, Mengyuan; Wang, Xin; Chen, Hong; Ren, Wenjie; Zhang, Yixin; Wang, Xiaoyun; Zhou, Guangdong

doi:10.1016/j.mtbio.2022.100489

Cited by 18 publications

(13 citation statements)

References 51 publications

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“…The expansion of BMSCs was performed using previously reported protocols. [61] Cytotoxicity Tests: To assess the cytotoxicity of GH and sGH, BMSCs were cultured at a density of 3 × 10 4 mL −1 in the previously described culture medium containing 1% gel precursors. The cell proliferation was assessed after 4 h and 1, 5, and 7 days using the Cell Counting Kit-8 (Dojindo, Japan).…”

Section: Methodsmentioning

confidence: 99%

Tolerant and Rapid Endochondral Bone Regeneration Using Framework‐Enhanced 3D Biomineralized Matrix Hydrogels

Bai,

Liu,

Huang

et al. 2023

Advanced Science

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Tissue‐engineered bone has emerged as a promising alternative for bone defect repair due to the advantages of regenerative bone healing and physiological functional reconstruction. However, there is very limited breakthrough in achieving favorable bone regeneration due to the harsh osteogenic microenvironment after bone injury, especially the avascular and hypoxic conditions. Inspired by the bone developmental mode of endochondral ossification, a novel strategy is proposed for tolerant and rapid endochondral bone regeneration using framework‐enhanced 3D biomineralized matrix hydrogels. First, it is meticulously designed 3D biomimetic hydrogels with both hypoxic and osteoinductive microenvironment, and then integrated 3D‐printed polycaprolactone framework to improve their mechanical strength and structural fidelity. The inherent hypoxic 3D matrix microenvironment effectively activates bone marrow mesenchymal stem cells self‐regulation for early‐stage chondrogenesis via TGFβ/Smad signaling pathway due to the obstacle of aerobic respiration. Meanwhile, the strong biomineralized microenvironment, created by a hybrid formulation of native‐constitute osteogenic inorganic salts, can synergistically regulate both bone mineralization and osteoclastic differentiation, and thus accelerate the late‐stage bone maturation. Furthermore, both in vivo ectopic osteogenesis and in situ skull defect repair successfully verified the high efficiency and mechanical maintenance of endochondral bone regeneration mode, which offers a promising treatment for craniofacial bone defect repair.

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Section: Methodsmentioning

confidence: 99%

Tolerant and Rapid Endochondral Bone Regeneration Using Framework‐Enhanced 3D Biomineralized Matrix Hydrogels

Bai,

Liu,

Huang

et al. 2023

Advanced Science

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“…The CAM alone, Cx-CAM-GA(0.6), Cx-CAM-PEG(0.6), Cx-CAM-PEG(1), Cx-CAM-PEG(3), and Cx-CAM-PEG(5) films were loaded on shaker and washed alternately three times with DW and PBS and finally dried at 37 °C for 12 h. Then Cx-CAM-GA(0.6), Cx-CAM-PEG(0.6), Cx-CAM-PEG(1), Cx-CAM-PEG(3), and Cx-CAM-PEG (5) were pulverized in the same procedure as described for CAM powder preparation. The obtained powders were dispersed in a 15% tween-80 solution at a concentration of 1 mg mL −1 , and their particle size distribution was measured using a particle size analyzer (ELSZ-1000; Otsuka Electronics, Osaka, Japan) in accordance with the manufacturer's instructions.…”

Section: Preparation Of Injectable Cx-cam-peg Suspensionsmentioning

confidence: 99%

“…[4] Additionally, because it is a cartilage-derived tissue, the CAM responds to tensile and compressive forces that are experienced by the cartilage. [5,6] Drugs and/or cells can be mixed in injectable hydrogels in a lossless manner, and scaffolds can be easily formed by injection. [7][8][9] Several biomaterials such as synthetic and natural materials have been used to fabricate injectable hydrogels.…”

Section: Introductionmentioning

confidence: 99%

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Development and In Vivo Assessment of an Injectable Cross‐Linked Cartilage Acellular Matrix‐PEG Hydrogel Scaffold Derived from Porcine Cartilage for Tissue Engineering

Kim

et al. 2023

Macromolecular Bioscience

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The cartilage acellular matrix (CAM) derived from porcine cartilage, which does not induce significant inflammation and provides an environment conducive for cell growth and differentiation, is a promising biomaterial candidate for scaffold fabrication. However, the CAM has a short period in vivo, and the in vivo maintenance is not controlled. Therefore, this study is aimed at developing an injectable hydrogel scaffold using a CAM. The CAM is cross‐linked with a biocompatible polyethylene glycol (PEG) cross‐linker to replace typically used glutaraldehyde (GA) cross‐linker. The cross‐linking degree of cross‐linked CAM by PEG cross‐linker (Cx‐CAM‐PEG) according to the ratios of the CAM and PEG cross‐linker is confirmed by contact angle and heat capacities measured by differential scanning calorimetry. The injectable Cx‐CAM‐PEG suspension exhibits controllable rheological properties and injectability. Additionally, injectable Cx‐CAM‐PEG suspensions with no free aldehyde group are formed in the in vivo hydrogel scaffold almost simultaneously with injection. In vivo maintenance of Cx‐CAM‐PEG is realized by the cross‐linking ratio. The in vivo formed Cx‐CAM‐PEG hydrogel scaffold exhibits certain host–cell infiltration and negligible inflammation within and near the transplanted Cx‐CAM‐PEG hydrogel scaffold. These results suggest that injectable Cx‐CAM‐PEG suspensions, which are safe and biocompatible in vivo, represent potential candidates for (pre‐)clinical scaffolds.

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“…The scaffold contained a biomimetic cartilage microenvironment composed of decellularized extracellular matrix hydrogel derived from photo‐crosslinked cartilage and a biomimetic bone microenvironment composed of a combination of photo dECM hydrogel and decalcified bone matrix. [ 281 ]…”

Section: The Biomedical Application Of Pchsmentioning

confidence: 99%

Photo‐Controllable Smart Hydrogels for Biomedical Application: A Review

Zhao,

Ran,

Lee

et al. 2023

Small Methods

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Nowadays, smart hydrogels are being widely studied by researchers because of their advantages such as simple preparation, stable performance, response to external stimuli, and easy control of response behavior. Photo‐controllable smart hydrogels (PCHs) are a class of responsive hydrogels whose physical and chemical properties can be changed when stimulated by light at specific wavelengths. Since the light source is safe, clean, simple to operate, and easy to control, PCHs have broad application prospects in the biomedical field. Therefore, this review timely summarizes the latest progress in the PCHs field, with an emphasis on the design principles of typical PCHs and their multiple biomedical applications in tissue regeneration, tumor therapy, antibacterial therapy, diseases diagnosis and monitoring, etc. Meanwhile, the challenges and perspectives of widespread practical implementation of PCHs are presented in biomedical applications. This study hopes that PCHs will flourish in the biomedical field and this review will provide useful information for interested researchers.

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Fabrication of biphasic cartilage-bone integrated scaffolds based on tissue-specific photo-crosslinkable acellular matrix hydrogels

Cited by 18 publications

References 51 publications

Tolerant and Rapid Endochondral Bone Regeneration Using Framework‐Enhanced 3D Biomineralized Matrix Hydrogels

Tolerant and Rapid Endochondral Bone Regeneration Using Framework‐Enhanced 3D Biomineralized Matrix Hydrogels

Development and In Vivo Assessment of an Injectable Cross‐Linked Cartilage Acellular Matrix‐PEG Hydrogel Scaffold Derived from Porcine Cartilage for Tissue Engineering

Photo‐Controllable Smart Hydrogels for Biomedical Application: A Review

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