Fabrication of Blended Gelatin–Polyvinyl Alcohol–Chitosan Scaffold for Wound Regeneration

Abstract

A single type polymer had very limited function and property which was not enough to be applied in the complex real situation. This study aimed to prepare the composited scaffolds by blending the gelatin, polyvinyl alcohol and chitosan together. The blended scaffolds were fabricated by making the final concentration of 7% gelatin, 0.5 % PVA and 0.1% chitosan and crosslinking by glutaraldehyde. The Young's modulus was investigated by using atomic force microscopy (AFM). The pore size was investigated using the scanning electron microscope (SEM). The swelling rate of scaffolds were tested by water displacement method. The degradation rate of the scaffolds was studied using lysozyme digestion. The MTT assay was applied within this study in order to find out the relative cell viability upon culturing with the gelatin and the blended scaffolds compared to tissue culture plates. Collagen type IV expression was investigated in mouse fibroblasts cultured on both scaffolds for 10 days using real time PCR. The results showed that Young's moduli of gelatin and blended scaffold were 53.30±26.80 kPa and 98.01±17.50 kPa, respectively. The average pore size of gelatin and blended scaffolds were 336.33±52.25 μm and 68.17±8.91 μm, respectively. The sample's porosity of gelatin and blended scaffolds were 85. 41±2. 11% and 21. 48±1. 01% , respectively. The swelling rate and the degradation rate of gelatin scaffold were higher than blended scaffold. The MTT

Chitosan/Gelatin/PVA Scaffolds for Beta Pancreatic Cell Culture

“…This process was continued until reaching the constant weight in three repeating measurements. The swelling percentage (SP) was calculated according to Equation (4) [ 39 ]: where W s is the weight of swollen scaffolds samples (mg), and W d is the weight of dry scaffolds samples (mg). The measurements were repeated three times for each type of scaffold.…”

“…The samples were weighed on day 3, 7, 14, 21 and 28. The percentage degradation (DP%) was expressed as degradation in percentage (%) of original weight samples using equation Equation ( ) [ 39 ]: where W t is the final weight scaffolds samples (mg), and W d is the weight of dry scaffolds samples (mg). The measurements were repeated three times for each type of scaffold.…”

“…This process was continued until reaching the constant weight in three repeating measurements. The swelling percentage (SP) was calculated according to Equation (4) [39]:…”

“…The samples were weighed on day 3, 7, 14, 21 and 28. The percentage degradation (DP%) was expressed as degradation in percentage (%) of original weight samples using equation Equation ( 5) [39]:…”

The Potential of Fibroblast Transdifferentiation to Neuron Using Hydrogels

“…Moreover, GA residuals can be harmful to the cultured cells. This problem was solved by soaking hydrogels in water before lyophilization to remove all GA residuals [52,53]. Various methods and principles about hydrogel crosslinking were provided by Hu et al [54].…”