Fabrication of boronate‐decorated polyhedral oligomeric silsesquioxanes grafted cotton fiber for the selective enrichment of nucleosides in urine

Abstract

Various cotton fiber based boronate-affinity adsorbents are recently developed for the sample pretreatment of cis-diol-containing biomolecules, but most do not have efficient capacity due to limited binding sites on the surface of cotton fibers. To increase the density of boronate groups on the surface of cotton fiber, polyhedral oligomeric silsesquioxanes were used to modify cotton fiber to provide plentiful reactive sites for subsequent functionalization with 4-formylphenylboronic acid. The new adsorbent showed special recognition ability towards cis-diols and high adsorption capacity (175 μg/g for catechol, 250 μg/g for dopamine, 400 μg/g for adenosine). The in-pipette-tip solid-phase extraction was investigated under different conditions, including pH and ionic strength of solution, adsorbent amount, pipette times, washing solvent, and elution solvent. The in-pipette-tip solid-phase extraction coupled with high-performance liquid chromatography was used to analyze four nucleosides in urine samples. Under the optimal extraction conditions, the detection limits were determined to be between 5.1 and 6.1 ng/mL (S/N = 3), and the linearity ranged from 20 to 500 ng/mL for these analytes. The accuracy of the analytical method was examined by studying the relative recoveries of analytes in real urine samples with recoveries varying from 83 to 104% (RSD = 3.9-10.2%, n = 3).

Facile preparation of a high‐capacity boronate‐affinity adsorbent based on low‐cost commercial supports for selective enrichment of cis‐diol‐containing biomolecules

“…Figure (inset plot) shows the Langmuir fitting curve; the maximum adsorption capacity of PGMA@FPBA adsorbent was calculated to be 448 μmol/g (Table S2). Compared with other boronate‐affinity adsorbents (Table ), the PGMA@FPBA was prepared with only one step and exhibited a higher binding capacity than the boronate‐affinity materials, except for one boronate‐affinity material . Moreover, the adsorption equilibrium was achieved within 1 min, indicating faster adsorption kinetics than other reported boronate‐affinity materials.…”

Returning to Nature for the Design of Sorptive Phases in Solid-Phase Microextraction

“…Furthermore, cotton fibers have been treated with 4-formylphenylboronic acid to obtain a boronate affinity adsorbent for the determination of nucleosides from urine samples. In this case, the microextraction process was simplified drawing upon the in-pipette-tip solid phase extraction (SPE), exhibiting good selectivity, capacity, and accuracy [46]. On the other hand, cotton has been altered through graft copolymerization with polyacrylonitrile (PAN) for selective extraction via the imprinting process of copper ions from aqueous solutions [47].…”

A Carry‐On Kit Containing Electrospun Nanofibrous Affinity Membranes by Surface Grafting Phenylboronic Acid for Quantitative Enrichment of Nucleotides in Urine

“…Therefore, it is essential to develop reliable and efficient sample pretreatment methods for the enrichment and determination of nucleosides and other cis ‐diol‐containing compounds in biological fluids. At present, in the research progress of nucleoside detection, the analytical methods mainly include high performance liquid chromatography, [6] liquid chromatography‐mass spectrometry, [7,8] capillary electrophoresis, [9,10] boric acid affinity technology based on adsorbent, [11–15] etc, and their sensitivities are generally in the range of 0.002–10 μg ml −1 .…”