Fabrication of chitosan/alginate/hydroxyapatite hybrid scaffolds using 3D printing and impregnating techniques for potential cartilage regeneration

Sadeghianmaryan, Ali; Naghieh, Saman; Yazdanpanah, Zahra; Sardroud, Hamed Alizadeh; Sharma, Nitin Kumar; Wilson, Lee D.; Xiongbiao, Chen

doi:10.1016/j.ijbiomac.2022.01.201

Cited by 86 publications

(51 citation statements)

References 54 publications

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“…To date, bioceramics have been largely used to help repair and reconstruct diseased or damaged living tissues and organs of the body ( Baino et al, 2015 ; Sadeghianmaryan et al, 2022 ). The use of bioactive ceramics in combination with polymers in BTE has gained interest as the resulting constructs possess bioactivity and high compressive strength/moduli provided by the ceramic phase while the polymeric network provides toughness, flexibility, and biodegradability ( Huang et al, 2018 ; Kumar et al, 2019 ).…”

Section: Bone Structure and Defectsmentioning

confidence: 99%

3D Bioprinted Scaffolds for Bone Tissue Engineering: State-Of-The-Art and Emerging Technologies

Yazdanpanah

Johnston

Cooper

et al. 2022

Front. Bioeng. Biotechnol.

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Treating large bone defects, known as critical-sized defects (CSDs), is challenging because they are not spontaneously healed by the patient’s body. Due to the limitations associated with conventional bone grafts, bone tissue engineering (BTE), based on three-dimensional (3D) bioprinted scaffolds, has emerged as a promising approach for bone reconstitution and treatment. Bioprinting technology allows for incorporation of living cells and/or growth factors into scaffolds aiming to mimic the structure and properties of the native bone. To date, a wide range of biomaterials (either natural or synthetic polymers), as well as various cells and growth factors, have been explored for use in scaffold bioprinting. However, a key challenge that remains is the fabrication of scaffolds that meet structure, mechanical, and osteoconductive requirements of native bone and support vascularization. In this review, we briefly present the latest developments and discoveries of CSD treatment by means of bioprinted scaffolds, with a focus on the biomaterials, cells, and growth factors for formulating bioinks and their bioprinting techniques. Promising state-of-the-art pathways or strategies recently developed for bioprinting bone scaffolds are highlighted, including the incorporation of bioactive ceramics to create composite scaffolds, the use of advanced bioprinting technologies (e.g., core/shell bioprinting) to form hybrid scaffolds or systems, as well as the rigorous design of scaffolds by taking into account of the influence of such parameters as scaffold pore geometry and porosity. We also review in-vitro assays and in-vivo models to track bone regeneration, followed by a discussion of current limitations associated with 3D bioprinting technologies for BTE. We conclude this review with emerging approaches in this field, including the development of gradient scaffolds, four-dimensional (4D) printing technology via smart materials, organoids, and cell aggregates/spheroids along with future avenues for related BTE.

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Section: Bone Structure and Defectsmentioning

confidence: 99%

3D Bioprinted Scaffolds for Bone Tissue Engineering: State-Of-The-Art and Emerging Technologies

Yazdanpanah

Johnston

Cooper

et al. 2022

Front. Bioeng. Biotechnol.

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“…From a biological point of view, an increase in terms of cell proliferation and collagen deposition, which affected the biomechanical behavior, was observed, suggesting the obtained 3D-bioprinted scaffold’s suitability for CTE. By coupling 3D printing and impregnating techniques, Sadeghianmaryan et al [ 62 ] developed a hybrid CS/ALG scaffold with nano hydroxyapatite (nHAp). The inclusion of nHAp increased the mechanical properties of scaffolds.…”

Section: Naturally-derived Bioinks For 3d Bioprinting Of Cartilage Ti...mentioning

confidence: 99%

Three-Dimensional Bioprinting for Cartilage Tissue Engineering: Insights into Naturally-Derived Bioinks from Land and Marine Sources

Szychlinska

Bucchieri

Fucarino

et al. 2022

JFB

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In regenerative medicine and tissue engineering, the possibility to: (I) customize the shape and size of scaffolds, (II) develop highly mimicked tissues with a precise digital control, (III) manufacture complex structures and (IV) reduce the wastes related to the production process, are the main advantages of additive manufacturing technologies such as three-dimensional (3D) bioprinting. Specifically, this technique, which uses suitable hydrogel-based bioinks, enriched with cells and/or growth factors, has received significant consideration, especially in cartilage tissue engineering (CTE). In this field of interest, it may allow mimicking the complex native zonal hyaline cartilage organization by further enhancing its biological cues. However, there are still some limitations that need to be overcome before 3D bioprinting may be globally used for scaffolds’ development and their clinical translation. One of them is represented by the poor availability of appropriate, biocompatible and eco-friendly biomaterials, which should present a series of specific requirements to be used and transformed into a proper bioink for CTE. In this scenario, considering that, nowadays, the environmental decline is of the highest concerns worldwide, exploring naturally-derived hydrogels has attracted outstanding attention throughout the scientific community. For this reason, a comprehensive review of the naturally-derived hydrogels, commonly employed as bioinks in CTE, was carried out. In particular, the current state of art regarding eco-friendly and natural bioinks’ development for CTE was explored. Overall, this paper gives an overview of 3D bioprinting for CTE to guide future research towards the development of more reliable, customized, eco-friendly and innovative strategies for CTE.

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“…The human body provides an impressive potential for tissue regeneration; however, this potential is limited by factors including tissue type and the requirement for growth hormones for differentiation, and physical size (crucial defect) [ 1 , 2 , 3 ]. Any damage to a tissue bigger than this crucial size requires the utilization of alternative aid, which has led to the development of tissue engineering (TE) and/or regenerative medicine (RM).…”

Section: Introductionmentioning

confidence: 99%

“…The synthesis of inks is critical to the success of 3D printing scaffolds for TE. An ink should have appropriate properties, including printability, mechanical properties (or stability), and biological properties (including degradation/insolubility in physiological solutions, cytocompatibility, and non-immunogenicity [ 1 , 2 , 3 , 25 ]). Despite numerous studies that have been reported on ink synthesis and optimization [ 26 , 27 , 28 , 29 , 30 , 31 ] for printing, the antibacterial behavior of inks has barely been investigated and documented.…”

Section: Introductionmentioning

confidence: 99%

A Review on Antibacterial Biomaterials in Biomedical Applications: From Materials Perspective to Bioinks Design

et al. 2022

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In tissue engineering, three-dimensional (3D) printing is an emerging approach to producing functioning tissue constructs to repair wounds and repair or replace sick tissue/organs. It allows for precise control of materials and other components in the tissue constructs in an automated way, potentially permitting great throughput production. An ink made using one or multiple biomaterials can be 3D printed into tissue constructs by the printing process; though promising in tissue engineering, the printed constructs have also been reported to have the ability to lead to the emergence of unforeseen illnesses and failure due to biomaterial-related infections. Numerous approaches and/or strategies have been developed to combat biomaterial-related infections, and among them, natural biomaterials, surface treatment of biomaterials, and incorporating inorganic agents have been widely employed for the construct fabrication by 3D printing. Despite various attempts to synthesize and/or optimize the inks for 3D printing, the incidence of infection in the implanted tissue constructs remains one of the most significant issues. For the first time, here we present an overview of inks with antibacterial properties for 3D printing, focusing on the principles and strategies to accomplish biomaterials with anti-infective properties, and the synthesis of metallic ion-containing ink, chitosan-containing inks, and other antibacterial inks. Related discussions regarding the mechanics of biofilm formation and antibacterial performance are also presented, along with future perspectives of the importance of developing printable inks.

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Fabrication of chitosan/alginate/hydroxyapatite hybrid scaffolds using 3D printing and impregnating techniques for potential cartilage regeneration

Cited by 86 publications

References 54 publications

3D Bioprinted Scaffolds for Bone Tissue Engineering: State-Of-The-Art and Emerging Technologies

3D Bioprinted Scaffolds for Bone Tissue Engineering: State-Of-The-Art and Emerging Technologies

Three-Dimensional Bioprinting for Cartilage Tissue Engineering: Insights into Naturally-Derived Bioinks from Land and Marine Sources

A Review on Antibacterial Biomaterials in Biomedical Applications: From Materials Perspective to Bioinks Design

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